PDF
FLIP
Fecha Publicación: 2007-12-21
Cómo citar
Osorio, J. H. (2007). Oxidación de un sustrato tritiado por linfocitos para el diagnóstico rápido de alteraciones metabólicas. Biosalud, 6, 19–24. Recuperado a partir de https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5841
Más formatos de cita
Autores/as
Resumen
Algunos métodos in vitro para el diagnóstico de alteraciones metabólicas son confiables, pero su realización consume mucho tiempo. El presente trabajo muestra una modificación substancial de la técnica de incubación de linfocitos en presencia de ácido palmítico tritiado, la cual reduce el tiempo de realización de la técnica de varios días a solo 6 horas. Estos cambios nos permiten la confirmación casi inmediata de los pacientes sospechosos de sufrir algunos errores innatos del metabolismo de los ácidos grasos.
Palabras clave:
Citas
Manning NJ, Olpin SE, Pollit R.J, Webley JA. Comparison of 9.10-3HPalmitic and 9.10-3Hmyristic acids for the detection of defects of fatty acid oxidation in intact cultured fibroblasts. J. Inher. Metab. Dis. 1990; 13:58-68.
Brivet M, Slama A, Saudubray JM, Legrand A, Lemonnier A. Rapid diagnosis of long chain and medium chain fatty acid oxidation disorders using lymphocytes. Ann Clin Biochem 1995; 32: 154-159.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol.Chem 1951; 193:265-275.
Olpin SE, Manning NJ, Carpenter K, Middleton B, Pollit RJ. Differential diagnosis of hydroxydicarboxylic aciduria based on release of 3H2O from [9,10-3H]-myristic and [9,10-3H]-palmitic acids by intact cultured fibroblasts. J. Inher. Metab. Dis. 1992;15:883-890.
Moon A, Rhead WJ. Complementation analysis of fatty acid oxidation disorders. J. Clin. Invest. 1987; 79:56-94.
Venizelos N, Von Dobeln U, Hagenfeld L. Fatty acid oxidation in fibroblasts from patients with defects in ß-oxidation and in the respiratory chain. J. Inher. Metab. Dis. 1998; 21:409-415
Ventura FV, Costa CG, Struys EA, Ruiter J, Allers P, Ijlst L, et al. Quantitative acylcarnitine profile in fibroblasts using U-13Cpalmitic acid: an improved tool for the diagnosis of fatty acid oxidation defects. Clin.Chem.Acta.1999; 281:1-17.
Brivet M, Slama A, Saudubray JM, Legrand A, Lemonnier A. Rapid diagnosis of long chain and medium chain fatty acid oxidation disorders using lymphocytes. Ann Clin Biochem 1995; 32: 154-159.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol.Chem 1951; 193:265-275.
Olpin SE, Manning NJ, Carpenter K, Middleton B, Pollit RJ. Differential diagnosis of hydroxydicarboxylic aciduria based on release of 3H2O from [9,10-3H]-myristic and [9,10-3H]-palmitic acids by intact cultured fibroblasts. J. Inher. Metab. Dis. 1992;15:883-890.
Moon A, Rhead WJ. Complementation analysis of fatty acid oxidation disorders. J. Clin. Invest. 1987; 79:56-94.
Venizelos N, Von Dobeln U, Hagenfeld L. Fatty acid oxidation in fibroblasts from patients with defects in ß-oxidation and in the respiratory chain. J. Inher. Metab. Dis. 1998; 21:409-415
Ventura FV, Costa CG, Struys EA, Ruiter J, Allers P, Ijlst L, et al. Quantitative acylcarnitine profile in fibroblasts using U-13Cpalmitic acid: an improved tool for the diagnosis of fatty acid oxidation defects. Clin.Chem.Acta.1999; 281:1-17.
Descargas
Los datos de descargas todavía no están disponibles.
