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Publication Date: 2007-12-21
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Osorio, J. H. (2007). Oxidation of a tritiated substrate by lymphocytes for the rapid diagnosis of metabolic errors. Biosalud, 6, 19–24. Retrieved from https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5841
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Abstract
Some methods for in vitro diagnosis of metabolic alterations are precise but time consuming. The present study shows a substantial modification for the technique of incubation of lymphocytes with tritiated palmitic acid reducing the spent time from some days to only 6 hours. These changes allow us the almost immediate confirmation of patients suspicious of suffering some inherited errors of fatty acid metabolism.
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References
Manning NJ, Olpin SE, Pollit R.J, Webley JA. Comparison of 9.10-3HPalmitic and 9.10-3Hmyristic acids for the detection of defects of fatty acid oxidation in intact cultured fibroblasts. J. Inher. Metab. Dis. 1990; 13:58-68.
Brivet M, Slama A, Saudubray JM, Legrand A, Lemonnier A. Rapid diagnosis of long chain and medium chain fatty acid oxidation disorders using lymphocytes. Ann Clin Biochem 1995; 32: 154-159.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol.Chem 1951; 193:265-275.
Olpin SE, Manning NJ, Carpenter K, Middleton B, Pollit RJ. Differential diagnosis of hydroxydicarboxylic aciduria based on release of 3H2O from [9,10-3H]-myristic and [9,10-3H]-palmitic acids by intact cultured fibroblasts. J. Inher. Metab. Dis. 1992;15:883-890.
Moon A, Rhead WJ. Complementation analysis of fatty acid oxidation disorders. J. Clin. Invest. 1987; 79:56-94.
Venizelos N, Von Dobeln U, Hagenfeld L. Fatty acid oxidation in fibroblasts from patients with defects in ß-oxidation and in the respiratory chain. J. Inher. Metab. Dis. 1998; 21:409-415
Ventura FV, Costa CG, Struys EA, Ruiter J, Allers P, Ijlst L, et al. Quantitative acylcarnitine profile in fibroblasts using U-13Cpalmitic acid: an improved tool for the diagnosis of fatty acid oxidation defects. Clin.Chem.Acta.1999; 281:1-17.
Brivet M, Slama A, Saudubray JM, Legrand A, Lemonnier A. Rapid diagnosis of long chain and medium chain fatty acid oxidation disorders using lymphocytes. Ann Clin Biochem 1995; 32: 154-159.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol.Chem 1951; 193:265-275.
Olpin SE, Manning NJ, Carpenter K, Middleton B, Pollit RJ. Differential diagnosis of hydroxydicarboxylic aciduria based on release of 3H2O from [9,10-3H]-myristic and [9,10-3H]-palmitic acids by intact cultured fibroblasts. J. Inher. Metab. Dis. 1992;15:883-890.
Moon A, Rhead WJ. Complementation analysis of fatty acid oxidation disorders. J. Clin. Invest. 1987; 79:56-94.
Venizelos N, Von Dobeln U, Hagenfeld L. Fatty acid oxidation in fibroblasts from patients with defects in ß-oxidation and in the respiratory chain. J. Inher. Metab. Dis. 1998; 21:409-415
Ventura FV, Costa CG, Struys EA, Ruiter J, Allers P, Ijlst L, et al. Quantitative acylcarnitine profile in fibroblasts using U-13Cpalmitic acid: an improved tool for the diagnosis of fatty acid oxidation defects. Clin.Chem.Acta.1999; 281:1-17.
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