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Abstract
The polymorphic variants of codon 72 of the tumor suppressor gene P53 (TP53) have been related with increased susceptibility to certain types of neoplasms, and for this reason we explored the possible relationship of these polymorphisms and interaction with demographic factors such as tobacco usage, alcohol consumption and socioeconomic stratification, with gastric cancer (GC) in a high risk Colombian population. Gastric cancer cases identified at the Hospital de Caldas in the city of Manizales, Colombia were collected for 18 months. Seventy-five patients were initially identified, from which 65 complied the inclusion criteria for the study. An EDTA peripheral blood sample was obtained from every participant. DNA was extracted with the salting out method and the P53 genotypification was determined for RFLP-PCR. No significant differences in the frequencies for variants of codon 72 of the tumor suppressor gene (TP53) between patients and controls were found (Arg/Arg=47.7; Arg/ Pro=38.5; Pro/Pro=13.8%; and Arg/Arg=49.2; Arg/Pro=41.5 and Pro/Pro=9.2, espectively), nor when the interaction with tobacco usage was estimated. These results did not identify an association between the polymorphism of codon 72 of the tumor suppressor gene P53, nor any interaction of these polymorphic variants, with tobacco usage, alcohol consumption and the risk of GC in this population. A higher effect is seen from tobacco usage and socioeconomic stratification on the GC risk.
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References
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