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Publication Date: 2009-12-18
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Romero Peñuela, M. H., & Sánchez Valencia, J. A. (2009). Use of recombinant antigens for serological evaluation of canine visceral Leishmaniasis and Tripanosomiasis. Biosalud, 8, 77–83. Retrieved from https://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/5526
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Abstract
A cross-sectional study was conducted in order to evaluate the seroprevalence of zoonotic visceral Leishmaniasis and American Trypanosomiasis in 211 mongrel dogs from an endemic area for the two protozoans in southern Tolima. Serum anti-Leishmania infantum and anti-Trypanosoma cruzi antibodies were determined by ELISA and Western Blot techniques, using rK39 and TESA recombinant antigens respectively. The Xenodiagnosis was performed on the seropositive to T. cruzi. According to the results, 40.28% (85/211) and 1.42% (3/211) of the dogs tested positive for antibodies against L. infantum and T. cruzi respectively. The Xenodiagnosis was negative. The results suggest that dogs may play an important role as reservoirs of L. infantum and infection sources for children in the study area. However, dogs may play a limited role in the transmission of T. cruzi. It is necessary to have an algorithm for diagnostic tests to confirm the actual status of the animals’ infection and to establish the presence of cross-reactions or co-infections.
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References
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Turriago BC, Vallejo GA, Guhl F. Seroprevalencia de Trypanosoma cruzi en perros de dos áreas endémicas de Colombia. Revista Med 2008;16(1):11-18.
Romero MH, López MC, Echeverry MC, Rivas F. Leishmaniasis visceral canina: pruebas diagnósticas no identifican estados reales de la infección. Rev. Salud pública 2008;10(2):290-298.
Montenegro V, Jiménez M, Pinto Dias JC, Zeledón R. Chagas disease in dogs from endemic areas of Costa Rica. Mem Inst Oswaldo Cruz 2002;97(4):491-494.
Courtenay O, Rupert J, Quinnell L, Garcez M, Shaw J, Dye D. Infectiousness in a Cohort of Brazilian dogs: Why culling fails to control visceral leishmaniasis in areas of high transmission. J Infect dis 2002;186:1314-1320.
Cabrera M A, Paula A, Camacho LA, Marzochi MC, Xavier SC, Da Silva AV, et al. Canine Visceral Leishmaniasis in Barra de Guaratiba, Rio de Janeiro, Brazil: Assesment of risk factors. Rev. Inst. Med. Trop. Sao Paulo 2003;45(2):79-83.
Alves WA, Bevilacqua PD. Quality of diagnosis of canine visceral Leishmaniasis in epidemiological surveys: an epidemic in Belo Horizonte, Minas Gerais, Brazil, 1993-1997. Cad. Saude Pública 2004;20(1):259-265.
Cardinal MV, Lauricella MA, Marcet PL, Orozco MM, Kitron U, Gütler RE. Impacto f community-based vector control on house infestation and Trypanosoma cruzi infection in Triatoma infestans, dogs and cats in the Argentine Chaco. Acta Trop. 2007;103(3):201-211.
Shadomy S, Waring SC, Chappell C. Combined use of Enzyme-linked Immunoabsorbent assay and flow cytometry to detect antibodies to Trypanosoma cruzi in domestic canines in Texas. Clin Diagn Lab. Immunol. 2004;3:313-319.
Umezawa E, Da Silveira JF. Serological diagnosis of Chagas disease with purified and defined Trypanosoma cruzi antigens. Mem. Inst. Oswaldo Cruz 1999;94(1):285-288.
Umezawa E, Luquetti A, Levitus G, Ponce C, Ponce E, Henríquez D, et al. Serodiagnosis of chronic and acute Chagas´ disease with Trypanosoma cruzi recombinant proteins: results of a collaborative study in six Latin American countries. J. Clin. Microbiol. 2004;42:449-452.
Luquetti AL, Ponce C, Ponce E, Esfandiari J, Schijman A, Revollo S, et al. Chagas disease diagnosis: a multicentric evaluation of Chagas stat-pak, a rapid immunochromatographic assay with recombinant proteins of Trypanosoma cruzi. Diag. Microbiol. Infect. Dis. 2003;46:267-271.
Fernández J, Charry T, Bello F, Escobar J, Lozano C, Ayala M, et al. Prevalencia de Leishmaniosis visceral canina en municipios de Huila, Colombia. Rev. Salud pública 2002;4(3):278-285.
Lauricella MA, Castanera MB, Gürtler RE, Segura EL. Immunodiagnosis of Trypanosoma cruzi (Chagas Disease) infection in naturally infected dogs. Mem. Inst. Oswaldo Cruz 1998;93(4):501-507.
Nakazawa M, Rosa D, Pereira V, Moura M, Furtado V, Souza W, et al. Excretory-secretory antigens of Trypanosoma cruzi are potentially useful for serodiagnosis of Chronic Chagas´ disease. Clin. Diag. Lab. Immunol. 2001;8(5):1024-1027.
Salotra P, Sreenivas G, Pogue G, Lee N, Nakhasi H, Armes V, Negi NS. Evaluation of enzyme-linked immunoabsorbent assay for diagnosis of Post-kalazar termal leishmaniasis with crude or recombinant k39 antigen. J. Clin. Microbiol. 2001;39(3):849-854.
Carvalho GS, Lemos EM, Corey R, Dítese R. Performance of recombinant K39 antigen in the diagnosis of Brazilian Visceral Leishmaniasis. Am. J. Trop. Med. Hyg. 2003;68(3):321-324.
Instituto Colombiano Agustín Codazzi. Diccionario Geográfico de Colombia. Bogotá, D.C.; 1996.
Travi B, Tabares CJ, Cadena H, Ferro C, Osorio Y. Canine visceral leishmaniasis in Colombia. Relationship between clinical and parasitologic status and infectivity for sand flies. J. Trop. Med. Hyg. 2001;64(3,4):119-124.
Téran-Angel G, Schallig H, Zerpa O, Rodríguez V, Ulrich M, Cabrera M. The direct agglutination test as an alternative method for the diagnosis of canine and human visceral Leishmaniasis. Biomédica 2007;27:447-53.
Braz R, Nascimento E, Martins D, Wilson M, Pearson RD, Reed S, Jeronimo S. The sensitivity and specificity of Leishmania chagasi recombinant k39 antigen in the diagnosis of American Visceral Leishmaniasis and in differentiating active from subclinical infection. Am. J. Trop. Med. Hyg. 2002;67(4):344-348.
Kumar P, Pai K, Tripathi K, Pandey H, Sundar S. Immunoblot Analysis of the humoral immune response to Leishmania donovani polypeptides in cases of human visceral Leishmaniasis: its usefulness in prognosis. Clin. Diag. Lab. Immunol. 2002;9:1119-1123.
Reithinger R, Quinnell R, Alexander B, Davies C. Rapid detection of Leishmania infantum in dogs: comparative study using immunochromatographic Dipstick Test, Enzyme-linked immunosorbent Assay, and PCR. J. Clin. Microbiol. 2002;40(7):2352-2356.
Gurtler R, Cohen J, Cecere M, Lauricella M, Chuit R, Segura E. Influence of humans and domestic animals on the household prevalence of Trypanosoma cruzi in Triatoma infestans populations in northwest Argentina. Am. J. Trop. Med. Hyg. 1998;58(6):748-758.
Diosque P, Padilla A, Cimino R, Cardozo R, Sánchez O, Marco J, Zacca R, et al. Chagas disease in rural areas of Chaco province Argentina: epidemiologic survey in humans, reservoirs, and vectors. Am. J. Trop. Med. Hyg. 2004;71(5):590-593.
Estrada-Franco JG, Bhatia V, Díaz-Albiter H, Ochoa-Gracía L, Barbosa A, Vázquez-Chagoyan JC, et al. Human Trypanosoma cruzi infection and seropositivity in dogs, Mexico. Emergeting Infection Disease 2006;12(4):624-630.
Crisante G, Rojas A, Teixeira MM, Añez N. Infected dogs as a risk factor in the transmission of human Trypanosoma cruzi infection in werstern Venezuela. Acta Trop. 2006;98(3):247-252.
Rojas ME, Várquez P, Villarreal MF, Velandia C, Vergara L, Morán-Borges YH, et al. Estudio seroepidemiológico y entomológico sobre la enfermedad de Chagas en un área infestada por Triatoma maculata (Erchson 1848) en el centro-occidente de Venezuela. Cad. Saúde Pública 2008;24(10):2323-2333.
Vallejo GA, Guhl F. Ciclos ecoepidemiológicos de Trypanosoma cruzi en Colombia. Biomédica 2009;29(Supl):97-103.
López DC, Jaramillo C, Guhl F. Estructura poblacional y variabilidad genética de Rhodnius prolixus (Hemíptera: Reduviidae) procedente de diferentes áreas geográficas de Colombia. Bomédica 2007;27(Supl 1):28-39.
Turriago BC, Vallejo GA, Guhl F. Seroprevalencia de Trypanosoma cruzi en perros de dos áreas endémicas de Colombia. Revista Med 2008;16(1):11-18.
Romero MH, López MC, Echeverry MC, Rivas F. Leishmaniasis visceral canina: pruebas diagnósticas no identifican estados reales de la infección. Rev. Salud pública 2008;10(2):290-298.
Montenegro V, Jiménez M, Pinto Dias JC, Zeledón R. Chagas disease in dogs from endemic areas of Costa Rica. Mem Inst Oswaldo Cruz 2002;97(4):491-494.
Courtenay O, Rupert J, Quinnell L, Garcez M, Shaw J, Dye D. Infectiousness in a Cohort of Brazilian dogs: Why culling fails to control visceral leishmaniasis in areas of high transmission. J Infect dis 2002;186:1314-1320.
Cabrera M A, Paula A, Camacho LA, Marzochi MC, Xavier SC, Da Silva AV, et al. Canine Visceral Leishmaniasis in Barra de Guaratiba, Rio de Janeiro, Brazil: Assesment of risk factors. Rev. Inst. Med. Trop. Sao Paulo 2003;45(2):79-83.
Alves WA, Bevilacqua PD. Quality of diagnosis of canine visceral Leishmaniasis in epidemiological surveys: an epidemic in Belo Horizonte, Minas Gerais, Brazil, 1993-1997. Cad. Saude Pública 2004;20(1):259-265.
Cardinal MV, Lauricella MA, Marcet PL, Orozco MM, Kitron U, Gütler RE. Impacto f community-based vector control on house infestation and Trypanosoma cruzi infection in Triatoma infestans, dogs and cats in the Argentine Chaco. Acta Trop. 2007;103(3):201-211.
Shadomy S, Waring SC, Chappell C. Combined use of Enzyme-linked Immunoabsorbent assay and flow cytometry to detect antibodies to Trypanosoma cruzi in domestic canines in Texas. Clin Diagn Lab. Immunol. 2004;3:313-319.
Umezawa E, Da Silveira JF. Serological diagnosis of Chagas disease with purified and defined Trypanosoma cruzi antigens. Mem. Inst. Oswaldo Cruz 1999;94(1):285-288.
Umezawa E, Luquetti A, Levitus G, Ponce C, Ponce E, Henríquez D, et al. Serodiagnosis of chronic and acute Chagas´ disease with Trypanosoma cruzi recombinant proteins: results of a collaborative study in six Latin American countries. J. Clin. Microbiol. 2004;42:449-452.
Luquetti AL, Ponce C, Ponce E, Esfandiari J, Schijman A, Revollo S, et al. Chagas disease diagnosis: a multicentric evaluation of Chagas stat-pak, a rapid immunochromatographic assay with recombinant proteins of Trypanosoma cruzi. Diag. Microbiol. Infect. Dis. 2003;46:267-271.
Fernández J, Charry T, Bello F, Escobar J, Lozano C, Ayala M, et al. Prevalencia de Leishmaniosis visceral canina en municipios de Huila, Colombia. Rev. Salud pública 2002;4(3):278-285.
Lauricella MA, Castanera MB, Gürtler RE, Segura EL. Immunodiagnosis of Trypanosoma cruzi (Chagas Disease) infection in naturally infected dogs. Mem. Inst. Oswaldo Cruz 1998;93(4):501-507.
Nakazawa M, Rosa D, Pereira V, Moura M, Furtado V, Souza W, et al. Excretory-secretory antigens of Trypanosoma cruzi are potentially useful for serodiagnosis of Chronic Chagas´ disease. Clin. Diag. Lab. Immunol. 2001;8(5):1024-1027.
Salotra P, Sreenivas G, Pogue G, Lee N, Nakhasi H, Armes V, Negi NS. Evaluation of enzyme-linked immunoabsorbent assay for diagnosis of Post-kalazar termal leishmaniasis with crude or recombinant k39 antigen. J. Clin. Microbiol. 2001;39(3):849-854.
Carvalho GS, Lemos EM, Corey R, Dítese R. Performance of recombinant K39 antigen in the diagnosis of Brazilian Visceral Leishmaniasis. Am. J. Trop. Med. Hyg. 2003;68(3):321-324.
Instituto Colombiano Agustín Codazzi. Diccionario Geográfico de Colombia. Bogotá, D.C.; 1996.
Travi B, Tabares CJ, Cadena H, Ferro C, Osorio Y. Canine visceral leishmaniasis in Colombia. Relationship between clinical and parasitologic status and infectivity for sand flies. J. Trop. Med. Hyg. 2001;64(3,4):119-124.
Téran-Angel G, Schallig H, Zerpa O, Rodríguez V, Ulrich M, Cabrera M. The direct agglutination test as an alternative method for the diagnosis of canine and human visceral Leishmaniasis. Biomédica 2007;27:447-53.
Braz R, Nascimento E, Martins D, Wilson M, Pearson RD, Reed S, Jeronimo S. The sensitivity and specificity of Leishmania chagasi recombinant k39 antigen in the diagnosis of American Visceral Leishmaniasis and in differentiating active from subclinical infection. Am. J. Trop. Med. Hyg. 2002;67(4):344-348.
Kumar P, Pai K, Tripathi K, Pandey H, Sundar S. Immunoblot Analysis of the humoral immune response to Leishmania donovani polypeptides in cases of human visceral Leishmaniasis: its usefulness in prognosis. Clin. Diag. Lab. Immunol. 2002;9:1119-1123.
Reithinger R, Quinnell R, Alexander B, Davies C. Rapid detection of Leishmania infantum in dogs: comparative study using immunochromatographic Dipstick Test, Enzyme-linked immunosorbent Assay, and PCR. J. Clin. Microbiol. 2002;40(7):2352-2356.
Gurtler R, Cohen J, Cecere M, Lauricella M, Chuit R, Segura E. Influence of humans and domestic animals on the household prevalence of Trypanosoma cruzi in Triatoma infestans populations in northwest Argentina. Am. J. Trop. Med. Hyg. 1998;58(6):748-758.
Diosque P, Padilla A, Cimino R, Cardozo R, Sánchez O, Marco J, Zacca R, et al. Chagas disease in rural areas of Chaco province Argentina: epidemiologic survey in humans, reservoirs, and vectors. Am. J. Trop. Med. Hyg. 2004;71(5):590-593.
Estrada-Franco JG, Bhatia V, Díaz-Albiter H, Ochoa-Gracía L, Barbosa A, Vázquez-Chagoyan JC, et al. Human Trypanosoma cruzi infection and seropositivity in dogs, Mexico. Emergeting Infection Disease 2006;12(4):624-630.
Crisante G, Rojas A, Teixeira MM, Añez N. Infected dogs as a risk factor in the transmission of human Trypanosoma cruzi infection in werstern Venezuela. Acta Trop. 2006;98(3):247-252.
Rojas ME, Várquez P, Villarreal MF, Velandia C, Vergara L, Morán-Borges YH, et al. Estudio seroepidemiológico y entomológico sobre la enfermedad de Chagas en un área infestada por Triatoma maculata (Erchson 1848) en el centro-occidente de Venezuela. Cad. Saúde Pública 2008;24(10):2323-2333.
Vallejo GA, Guhl F. Ciclos ecoepidemiológicos de Trypanosoma cruzi en Colombia. Biomédica 2009;29(Supl):97-103.
López DC, Jaramillo C, Guhl F. Estructura poblacional y variabilidad genética de Rhodnius prolixus (Hemíptera: Reduviidae) procedente de diferentes áreas geográficas de Colombia. Bomédica 2007;27(Supl 1):28-39.
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