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Mendoza Meza, D. L. ., Sánchez Catalán, K. ., & Saavedra Ahumada, S. . (2016). Caracterización bioquímica de extractos acuosos de ulomoides dermestoides (coleoptera, tenebrionidae): exploración de la inhibición dual de la lipoxigenasa (15-lox) y ciclooxigenasas (cox-1 y cox-2). Biosalud, 15(2), 37–54. https://doi.org/10.17151/biosa.2016.15.2.5
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Ulomoides dermestoides es usado en medicina tradicional oriental para aliviar enfermedades inflamatorias y como afrodisíaco. En Colombia, comunidades campesinas lo consumen para el tratamiento del asma. El propósito de este estudio fue obtener extractos acuosos del cuerpo entero de U. dermestoides y explorar su potencial para inhibir enzimas involucradas en la biosíntesis de reguladores de la inflamación, la 15-lipoxigenasa (15-LOX) y las ciclooxigenasas COX-1 y COX-2 (COXs). Los extractos se obtuvieron con tampón salino fosfato 0,01M (PBS) y bicarbonato de amonio 0,1M (NH4 HCO3 ), en presencia de un detergente (Tween-20 o Dodecilsulfato sódico, SDS). Posteriormente se determinó el contenido de proteínas totales solubles, el perfil electroforético y las actividades enzimáticas a 19 hidrolasas. Las inhibiciones 15-LOX y COXs se midieron por método colorimétrico y por inmunoensayo enzimático, respectivamente. El rendimiento más alto de proteínas se obtuvo con el tampón PBS/SDS (1,603 %p/p). Este extracto mostró 13 bandas electroforéticas mayoritarias, con tamaños entre 8,8 y 151,2 kDa; también, se demostraron actividades enzimáticafosfosfohidrolasas, peptidasas, esterasas, β-glucoronidasa, α-glucosidasa, N-acetil-β-glucosaminidasa y α-manosidasa. Todos los extractos mostraron actividad inhibitoria dual 15-LOX y COXs. Las inhibiciones 15-LOX y COX-1 más altas se obtuvieron con el extracto obtenido con PBS/SDS (15-LOX: 62,8±2,0%; COX-1: 88,5±3,9%). Adicionalmente, un análisis de regresión lineal simple mostró que no hay asociación entre la concentración de proteínas y la inhibición 15-LOX (valor p = 0,493), este resultado sugiere que la actividad antilipooxigenasa podría ser debido a compuestos no proteicos.
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7. Shin K, Hwang JJ, Kwon BI, Kheradmand F, Corry DB, Lee SH. Leukotriene enhanced allergic lung inflammation through induction of chemokine production. ClinExp Med. 2015; 15(3):233-44. doi: 10.1007/s10238-014-0292-7.
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20. Mendoza DL, Maury C. Evaluación del contenido de fenoles totales y actividad captadora de radicales libres de extractos hidrometanólicos del escarabajo Ulomoides dermestoides (coleoptera: tenebrionidae). Rev. Asoc. Col. Cienc.(Col.). 2013; 25:135-41. Disponible en: http://www. asociacioncolombianadecienciasbiologicas.org/download/revistas/2013/art%2013.pdf.
21. Kim J, Jung BH. Contribution to the Tribes Diaperini Doyen in Korea (Coleoptera: Tenebrionidae: Diaperinae). Entomological Research. 2005; 35(2):95-100. doi: 10.1111/j.1748-5967.2005. tb00142.x.
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27. Zhao F1, Bi X, Hao Y, Liao X. Induction of viable but non culturable Escherichia coli O157: H7 by high pressure CO2 and its characteristics. PLoS One. 2013; 8(4):e62388. doi: 10.1371/journal.pone.0062388.
28. Budzyńska A, Sadowska B, Więckowska-Szakiel M, Różalska B. Enzymatic profile, adhesive and invasive properties of Candida albicans under the influence of selected plant essential oils. ActaBiochim Pol. 2014; 61(1):115-21. Disponible en: http://www.actabp.pl/pdf/1_2014/115.pdf.
29. Carnés J, Boquete M, Carballada FJ, Gallego MT, Fernández-Caldas E. Enzymatic activity in body and fecal extracts of the storage mite Chortoglyphus arcuatus. Int Arch Allergy Immunol. 2008; 145(3): 207-212.doi:10.1159/000109289.
30. Morales M, Iraola V, Leonor J, Carnés J. Enzymatic Activity of Allergenic House Dust and Storage Mite Extracts. J Med Entomol. 2013. 50(1): 147-54. doi: http://dx.doi.org/10.1603/ME12154.
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34. O’Neil S, Heinrich T, Hales B, Hazell L, Holt D, Fischer K et al. The chitinase allergens Der p15 and Der p18 from Dermatophagoide spteronyssinus. ClinExp Allergy 2006; 36(6):831-9. doi:10.1111/j.1365-2222.2006.02497.x.
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40. Kohyama N, Nagata T, Fujimoto S, Sekiya K.Inhibition of arachidonatelipoxygenase activities by 2-(3, 4-dihydroxyphenyl) ethanol, a phenolic compound from olives. BiosciBiotechnolBiochem. 1997; 61(2):347-50. doi: 10.1271/bbb.61.347.
41. Landa P, Kutil Z, Malik J, Kokoska L, Widowitz U, Marsik P, Bauer R, Vanek T. In vitro inhibition of 5-lipoxygenase by natural quinone compounds. Planta Med 2011; 77-102. doi: 10.1055/s-0031-1282860.
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2. Crusz SM, Balkwill FR. Inflammation and cancer: advances and new agents. Nat Rev ClinOncol. 2015; 12(10):584-96. doi:10.1038/nrclinonc.2015.105.
3. Medzhitov R1. Origin and physiological roles of inflammation. Nature. 2008; 454(7203):428-35. doi: 10.1038/nature07201.
4. Kotas ME, Medzhitov R. Homeostasis, inflammation, and disease susceptibility. Cell. 2015; 160(5):816- 27. doi: 10.1016/j.cell.2015.02.010.
5. Murakami M, Kudo I. Prostaglandin E synthase: a novel drug target for inflammation and cancer. Curr Pharm Des. 2006; 12(8):943-54.doi: 10.2174/138161206776055912.
6. Wisastra R, Dekker FJ. Inflammation, cancer and oxidative lipoxygenase activity are intimately linked. Cancers. 2014; 6:1500-21. doi: 10.3390/cancers6031500.
7. Shin K, Hwang JJ, Kwon BI, Kheradmand F, Corry DB, Lee SH. Leukotriene enhanced allergic lung inflammation through induction of chemokine production. ClinExp Med. 2015; 15(3):233-44. doi: 10.1007/s10238-014-0292-7.
8. Marnett LJ, Rowlinson SW, Goodwin DC, Kalgutkar AS, Lanzo CA. Arachidonic acid oxygenation by COX-1 and COX-2. Mechanisms of catalysis and inhibition. J Biol Chem. 1999; 274(33): 22903-6. doi: 10.1074/jbc.274.33.22903.
9. FAO Regional Office for Asia and the Pacific. Forest insects as food: humans bite back. Proceedings of a workshop on Asia-Pacific resources and their potential for development. Bangkok, Thailand. 2010; 151-60 pp. Disponible en: www.fao.org/docrep/012/i1380e/i1380e00.pdf.
10. Chu GS, Palmieri JR, Sullivan JT. Beetle-eating: a Malaysian folk medical practice and its public health implications. Trop Geogr Med. 1977; 29(4):422-427. PMID: 610028.
11. Sandroni P. Aphrodisiacs past and present: a historical review. ClinAuton Res. 2001; 11(5):303-7. doi: 10.1007/BF02332975.
12. Costa Neto E, Ramos-Elorduy J. Los insectos comestibles de Brasil: etnicidad, diversidad e importancia en la alimentación. Boletín Sociedad Entomológica Aragonesa. 2006; 38:423-42.
13. Crespo R, Villaverde ML, Girotti JR, Güerci A, Juárez MP, de Bravo MG. Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells. J Ethnopharmacol. 2011; 136(1):204- 9. doi: 10.1016/j.jep.2011.04.056.
14. Deloya-Brito G, Deloya C. Sustancias producidas por el coleóptero Ulomoides dermestoides (Chevrolat, 1878) (Insecta: Coleoptera: Tenebrionidae): efecto anti-inflamatorio y citotóxico. Acta ZoolMex. 2014; 30(3): 665-61. Disponible en: http://www.scielo.org.mx/pdf/azm/v30n3/v30n3a14.pdf.
15. Amat G, Devia N. Estos escarabajos curan el asma. Publicación de la Unidad de Medios de Comunicación de la Universidad Nacional de Colombia (UNIMEDIOS). Bogotá, Colombia. Disponible en: http://historico.unperiodico.unal.edu.co/ediciones/103/17.html. Consultado Junio 2015.
16. Santos RC, Lunardelli A, Caberlon E, Bastos CM, Nunes FB, Pires MG, Biolchi V, Paul EL, Vieira FB, Resende do Carmo Aquino A, Corseuil E, de Oliveira JR. Anti-inflammatory and immunomodulatory effects of Ulomoides dermestoides on induced pleurisy in rats and lymphoproliferation in vitro. Inflammation. 2010; 33(3):173-9. doi: 10.1007/s10753-009-9171-x.
17. Tobón FA, Gutiérrez GP, Mejía ML. Evaluación del perfil neurofarmacológico del aceite de Ulomoides dermestoides (Coleoptera: Tenebrionidae). Revista Colombiana de Entomología. 2011; 37(2):251-5. Disponible en: http://www.scielo.org.co/pdf/rcen/v37n2/v37n2a16.pdf.
18. Yan S, Chu Z. Antioxidant effects of delipidized protein hydrolysate from Martianus dermestoides Chevrolat (Coleoptera, Tenebrionidae) beetles. Acta Entomológica Sinica. 2008; 51(8): 804-9. Disponible en: http://www.insect.org.cn/EN/abstract/abstract9541.shtml#.
19. ShanChun Y, Lei W, Qing L, Yong F. Anti-senile effects of water extraction of Martianus dermestoides (Coleoptera: Tenebrionidae) feeding different foods on aging mice. Acta Entomol Sin. 2009; 52(7): 820-4. Disponible en: http://www.insect.org.cn/EN/abstract/abstract9369.shtml#.
20. Mendoza DL, Maury C. Evaluación del contenido de fenoles totales y actividad captadora de radicales libres de extractos hidrometanólicos del escarabajo Ulomoides dermestoides (coleoptera: tenebrionidae). Rev. Asoc. Col. Cienc.(Col.). 2013; 25:135-41. Disponible en: http://www. asociacioncolombianadecienciasbiologicas.org/download/revistas/2013/art%2013.pdf.
21. Kim J, Jung BH. Contribution to the Tribes Diaperini Doyen in Korea (Coleoptera: Tenebrionidae: Diaperinae). Entomological Research. 2005; 35(2):95-100. doi: 10.1111/j.1748-5967.2005. tb00142.x.
22. Bradford M. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein Dye Binding. Analytical Biochemistry 1972; 72:248-54. doi: 10.1016/0003-2697(76)90527-3.
23. Sambrook J, Russell D.W. SDS-Polyacrylamide Gel Electrophoresis of Proteins. CSH Protocols. 2006. doi: 10.1101/pdb.prot4540.
24. Yu WG, Zhang BB, Shen YJ, Li Y, Tian YB, Jiang MH. Purification and Characterization of Superoxide Dismutase from Martianus dermestoides Chevrolat. Adv Mat Res. 2013; 773:336-41. doi: 10.4028/www.scientific.net/AMR.773.336.
25. Long D, Defu C, Beibei Z, Xiaocan L, Jia Y. Optimization of extraction conditions for superoxide dismutase from Martianus dermestoides. Journal of Northeast Forestry University. 2009; 37(4): 69-
70.Disponible en: http://en.cnki.com.cn/Article_en/CJFDTOTAL-DBLY200904024.htm
26. Le Maire M, Champeil P, Møller JV. Interaction of membrane proteins and lipids with solubilizing detergents. BiochimicaetBiophysicaActa (BBA) – Biomembranes. 2000; 1508(1–2):86–111.doi:10.1016/S0304-4157(00)00010-1.
27. Zhao F1, Bi X, Hao Y, Liao X. Induction of viable but non culturable Escherichia coli O157: H7 by high pressure CO2 and its characteristics. PLoS One. 2013; 8(4):e62388. doi: 10.1371/journal.pone.0062388.
28. Budzyńska A, Sadowska B, Więckowska-Szakiel M, Różalska B. Enzymatic profile, adhesive and invasive properties of Candida albicans under the influence of selected plant essential oils. ActaBiochim Pol. 2014; 61(1):115-21. Disponible en: http://www.actabp.pl/pdf/1_2014/115.pdf.
29. Carnés J, Boquete M, Carballada FJ, Gallego MT, Fernández-Caldas E. Enzymatic activity in body and fecal extracts of the storage mite Chortoglyphus arcuatus. Int Arch Allergy Immunol. 2008; 145(3): 207-212.doi:10.1159/000109289.
30. Morales M, Iraola V, Leonor J, Carnés J. Enzymatic Activity of Allergenic House Dust and Storage Mite Extracts. J Med Entomol. 2013. 50(1): 147-54. doi: http://dx.doi.org/10.1603/ME12154.
31. Kramer Kj, Aoki H. Chitinolytic enzymes from pupae of the red flour beetle, Tribolium castaneum. Comp BiochemPhysiol B Biochem Mol Biol. 1987; 86(3):613-21. http://dx.doi.org/10.1016/0305-0491(87)90457-3.
32. Merzendorfer H, Zimoch L. Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases. J Exp Biol. 2003; 206: 4393-441. doi: 10.1242/jeb.00709.
33. Chun Geun Lee. Chitin, Chitinases and Chitinase-like Proteins in Allergic Inflammation and Tissue Remodeling. Yonsei Med J. 2009; 50(1):22–30. doi: 10.3349/ymj.2009.50.1.22.
34. O’Neil S, Heinrich T, Hales B, Hazell L, Holt D, Fischer K et al. The chitinase allergens Der p15 and Der p18 from Dermatophagoide spteronyssinus. ClinExp Allergy 2006; 36(6):831-9. doi:10.1111/j.1365-2222.2006.02497.x.
35. Schultze-Werninghaus G, Zachgo W, Rotermund H, Wiewrodt R, Merget R, Wahl R, Burow G, zurStrassen R. Tribolium confusum (confused flour beetle, rice flour beetle) an occupational allergen in bakers: demonstration of IgE antibodies. Int Arch Allergy ApplImmunol. 1991; 94(1-4):371-2.doi:10.1159/000235407.
36. Alanko K, Tuomi T, Vanhanen M, Pajari-Backas M, Kanerva L, Havu K, Saarinen K, Bruynzeel DP. Occupational IgE-mediated allergy to Tribolium confusum (confused flour beetle). Allergy. 2000;55(9):879-82. doi: 10.1034/j.1398-9995.2000.00572.x.
37. Wahrendorf M, Wink M. Pharmacologically active natural products in the defence secretion of Palembus ocularis (Tenebrionidae, Coleoptera). J Ethnopharmacol. 2006; 106(1): 51-6. doi: 10.1016/j.jep.2005.12.007.
38. Skrzypczak-Jankun E, Chorostowska-Wynimko J, Selman SH, Jankun J. Lipoxygenases- A challenging problem in enzyme inhibition and drug development. CurrEnzymInhib. 2007; 3(2):119-132. doi:http://dx.doi.org/10.2174/157340807780598350.
39. Chedea VS, Jisaka M. Inhibition of Soybean Lipoxygenases-Structural and Activity Models for the LipoxygenaseIsoenzymes Family. INTECH Open Access Publisher. 2011. Disponible en: http://cdn.intechweb.org/pdfs/22600.pdf.
40. Kohyama N, Nagata T, Fujimoto S, Sekiya K.Inhibition of arachidonatelipoxygenase activities by 2-(3, 4-dihydroxyphenyl) ethanol, a phenolic compound from olives. BiosciBiotechnolBiochem. 1997; 61(2):347-50. doi: 10.1271/bbb.61.347.
41. Landa P, Kutil Z, Malik J, Kokoska L, Widowitz U, Marsik P, Bauer R, Vanek T. In vitro inhibition of 5-lipoxygenase by natural quinone compounds. Planta Med 2011; 77-102. doi: 10.1055/s-0031-1282860.
42. Deszcz L, Kozubek A. Inhibition of soybean lipoxygenases by resorcinolic lipids from cereal bran. Cell MolBiolLett. 1997; 2:213-22. Disponible en: http://www.ibmb.uni.wroc.pl/publak/soybean.pdf.
43. Kotsyfakis M, Sá-Nunes A, Francischetti IM, Mather TN, Andersen JF, Ribeiro JM. Antiinflammatory and immunosuppressive activity of sialostatin L, a salivary cystatin from the tick Ixodesscapularis. J Biol Chem. 2006; 281(36):26298-307. doi: 10.1074/jbc.M513010200.
44. Jurenka RA, Howard RW, Blomquist GJ. Prostaglandin synthetase inhibitors in insect defensive secretions. Naturwissenschaften. 1986; 73(12):735-7. doi: 10.1007/BF00399245.
45. Howard R, Jurenka R, Blomquist G. Prostaglandin synthetase inhibitors in the defensive secretion of the red flour beetle Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Insect biochemistry.1986; 16(5):757-60. doi: 10.1016/0020-1790(86)90111-3.
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