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Publication Date: 2010-01-01
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Romero Peñuela M, Sánchez Valencia J, Hayek Peñuela LC. Canine visceral leishmaniasis diagnosis by means of western blot and indirect immunofluorescence test. Hacia Promoc. Salud [Internet]. 2010 Jan. 1 [cited 2024 Nov. 23];15(1):55-62. Available from: https://revistasojs.ucaldas.edu.co/index.php/hacialapromociondelasalud/article/view/1971
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Abstract
Objective: a cross-sectional epidemiological study was carried out in order to establish the prevalence of canine visceral leishmaniasis in the endemic area of Tolima department by means of Indirect Immunofluorescence (IFAT) and Western Blotting (WB) tests. Materials and Methods: the study included 211 mongrel dogs from six municipalities of the endemic area of visceral leishmaniasis (VL) of the Tolima department. The Colombian Leishmania infantum MHOM/CO/CL044B strain was used as antigen for IFI and WB. Results: The seroprevalence in the group tested was 41.7% and 22.2% by IFAT and WB, respectively. The agreement between the two techniques was negative (k = -0.102; p = 0.084). 12 antigenic fractions were detected between 13 and 91 kDa, of which 14 kDa is considered valuable in early diagnosis of the infection, in order to evaluate the resolution of the disease and treatment progress. The article suggests developing a construct of direct and indirect diagnostic tests for confirming the real state of the animals infection to guide disease control programs in endemic areas of visceral leishmaniasis.
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References
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Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-685.
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Iniesta L, Fernández-Barredo S, Bulle B, Gómez M, Piarrous R, Gallego M, et al. Diagnostic techiniques to detect cryptic leishmaniasis in dogs. Clin Diagn Lab Immnunol 2002;9:1137-1141.
Lasri S, Sahibi H, Natami A, Rhalem A. Western blot analysis of Leishmania infantum antigens using sera from pentamidine-treated dogs. Vet Immunol Immunopathol 2003;91:13-18.
Aisa M, Castillejo S, Gallego M, Fisa R, Riera M, Colmenares M, et al. Diagnostic potential of western blot analysis of sera from dogs with leihsmaniasis in endemic areas and significance of the pattern. Am J Trop Med Hug 1998;58:154-159.
Mary C, Lamouroux D, Dunan S, Quillci M. Western blot analysis of antibodies to Leishmania infantum antigens: potential of the 14-Kd and 16-Kd antigens for diagnosis and epidemiological purposes. Am J Trop Med Hyg 1992;47:764-771.
Ferroglio, F, Centaro E, Mignone W, Trisciuoglio A. Evaluation o fan ELISA rapid device for the serological diagnosis of Leishmania infantum infection in dog as compared with immunofluorescence assay and Western blot. Vet. Parasitol. 2007;144:162-166.
Aoun O, Mary Ch, Roquedplo C, Marié JL, Terrier O, Levieuge A, et al. Canine leishmaniasis in Routh-east of France: Screening of Leishmania infantum antibodies (western blotting, ELISA) and parasitaemia levels by PCR quintification. Vet. Parasitol. 2009;166:27-31.
Corredor A, Álvarez CA, Agudelo CA, Bueno M, López MC, Cáceres E, et al. Prevalence of Trypanosoma cruzi and Leishmania chagasi infection and risk factors in a Colombian indigenous population. Rev. Inst. Med. Trop. Sao Paulo 1999;41(4):229-234.
Travi B. Un enfoque integral al estudio de animales reservorios, con énfasis en las Leishmaniosis del Nuevo mundo. Med UIS 1998;12:311-315.
Ministerio de la Protección Social de Colombia. Guía de Atención de la Leishmaniasis 21; 2006.
Travi B, Tabares C, Cadena H, Ferro C, Osorio Y. Canine Visceral Leishmaniasis in Colombia: relationship between clinical and parasitologic status and infectivity for sand flies. Am. J. Trop. Med. Hyg. 2001;64(3,4):119-124.
Fernández J, Charry T, Bello F, Escobar J, Lozano C, Ayala M, et al. Prevalencia de Leishmaniosis Visceral canina en municipios de Huila Colombia. Rev. Salud Pública 2002;4(3):278-85.
Mettler M, Grimm F, Capelli G, Camp H, Deplazes P. Evaluation of Enzyme-Linked Immunosorbent assays, an Immunofluorescent-antibody test, and two rapid tests (Immunochromatographic-Dipstick and gel tests) for serological diagnosis of symptomatic and asymptomatic Leishmania infections in dogs. J Clin Microbiol 2005;43(11):5515-157.
De Paula A, Da Silva A, Fernandes O, Jansen A. The use of immunoblot analysis in the diagnosis of canine visceral leishmaniasis in an endemic area of Rio de Janeiro. J Parasitol 2009;89:832-836.
Riera C, Valladares JE, Gallego M, Aisa M, Castillejo S, Fisa R, et al. Serological and parasitological follow-up in dogs experimentally infected with Leishmania infantum and treated with meglumine antimoniate. Vet. Parasitol. 1999;84:33-47.
Vargas-Duarte JJ, López-Páez MC, Escovar-Castro JE, Fernández-Manrique J. Evaluación por western blot, inmunofluorescencia indirecta y ELISA de perros infectados con Leishmania (Leishmania) infantum. Rev. Salud Pública 2009;11(4):641-652.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-685.
Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrilamide gels to nitrocellulose sheets: procedures and some applications. Proc Natl Acad Sci USA 1979;76:4350-4354.
Congreso de la República de Colombia. Ley 084 de 1989. Estatuto Nacional de Protección de los animales. Diario Oficial, Año CXXVI. N.3912027; 1989. p. 1-14.
Ministerio de Salud, República de Colombia. Resolución No. 008430 de 1993. Investigación en Salud; 1993. p. 1-20.
Fernández J, Bello F, López MC, Moncada LI, Vargas JJ, Ayala MS, et al. Seroprevalencia de leishmaniosis visceral canina en la comuna 8 de Neiva y en cuatro municipios de Huila, Colombia. Biomédica 2006;26(Supl.1):121-30.
Iniesta L, Fernández-Barredo S, Bulle B, Gómez M, Piarrous R, Gallego M, et al. Diagnostic techiniques to detect cryptic leishmaniasis in dogs. Clin Diagn Lab Immnunol 2002;9:1137-1141.
Lasri S, Sahibi H, Natami A, Rhalem A. Western blot analysis of Leishmania infantum antigens using sera from pentamidine-treated dogs. Vet Immunol Immunopathol 2003;91:13-18.
Aisa M, Castillejo S, Gallego M, Fisa R, Riera M, Colmenares M, et al. Diagnostic potential of western blot analysis of sera from dogs with leihsmaniasis in endemic areas and significance of the pattern. Am J Trop Med Hug 1998;58:154-159.
Mary C, Lamouroux D, Dunan S, Quillci M. Western blot analysis of antibodies to Leishmania infantum antigens: potential of the 14-Kd and 16-Kd antigens for diagnosis and epidemiological purposes. Am J Trop Med Hyg 1992;47:764-771.
