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Chaves-Velasquez, Ángela M., Guzmán-Piedrahita, Óscar A., Valencia-Jíménez, A., & Villegas-Estrada, B. (2023). Morphometric and molecular characterisation of populations of Radopholus similis [(Cobb) Thorne (Rhabditida: Pratylenchidae)] in Colombia. Boletín Científico. Centro De Museos, 27(2), 97–116. https://doi.org/10.17151/bccm.2023.27.2.6
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Abstract
Objective: To study intraspecific variability in different populations of Radopholus similis. Scope: Populations of R. similis mainly from the Bajo Occident of Caldas, Colombia. Methodology: Nineteen morphometric and allometric characters were measured in females and males of R. similis collected in the municipalities of Anserma, Belalcázar, Carepa and Palestina, which were analyzed by principal components and hierarchical clustering. The partial region ITS1-5.8S-ITS2 and the 18S ribosomal RNA gene corresponding to the aforementioned R. similis populations were also amplified by PCR and sequenced. With these sequences, and 61 additional sequences obtained from the NCBI database, a haplotypic network was constructed to detect genetic variation in the species. Main results: Morphometric characterization showed that R. similis females from Anserma and Belalcázar presented a more elongated esophagus and those collected from Palestina were more robust in the anterior region at the level of the stipe base, with more elongated bodies and esophagi. Molecular analysis of the ITS1-5.8S-ITS2 region of ribosomal RNA showed no intraspecific variability among the R. similis populations studied. However, the haplotypic network constructed in this study, together with that collected from the NCBI database, revealed intraspecific variability among R. similis populations from Africa and Southeast Asia. Conclusions: It was not possible to detect molecular variability among the R. similis populations studied. However, morphometric variability was found among R. similis populations from Anserma, Belalcázar, Carepa and Palestina, Colombia.
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References
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Arboleda, F., Guzmán, A. y Mejía, L. (2012). Efecto de extractos cetónicos de higuerilla sobre el nematodo barrenador en condiciones in vitro. Luna Azul. 35, 28-48.
Bird, A. F. y Bird, J. (2012). The structure of nematodes. Academic Press. Castillo-Russi, J., Araya-Vargas, M. y Patiño-Hoyos, L. (2010). Respuesta a la aplicación de nematicida en banano en la zona de Urabá, Colombia. Agronomía Mesoamericana, 21(2), 307-317.
Castresana, J. (2000). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution, 17(4), 540-552.
Cenibanano. (Centro de Investigaciones del Banano). (2018). Informe Cenibanano 2018.
CIAT (Centro Internacional de Agricultura Tropical). (2013). Producción de material de “siembra” limpio en el manejo de las enfermedades limitantes de plátano. CIAT.
Costa, D., Faleiro, G., Cares, J. y Gomes, A. (2008). Pathogenicity and genetic variability of Radopholus similis populations in bananas (Musa acuminata AAA and AA) based on RAPD analysis. Nematologia Brasileira Piracicaba (SP) Brasil. 32(4), 303-316.
Edgar, R. C. (2004). Muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res., 32(5),1792-1797
Elbadri, G. A., Geraert, E. y Moens, M. (1999). Morphological differences among Radopholus similis (Cobb, 1893) Thorne, 1949 populations. Russian Journal of Nematology, 7(2), 139-153.
Elbadri, A., De Ley, P., Waeyenberge, L., Vierstraete, A., Moens, M. y Vanfleteren, J. (2002). Intraspecific variation in Radopholus similis isolates assessed with restriction fragment length polymorphism and DNA sequencing of the internal transcribed spacer region of the ribosomal RNA cistron. International Journal for Parasitology, 32,199-205.
Fallas, G. A., Sarah, J. L. y Fargette, M. (1995). Reproductive fitness and pathogenicity of eight Radopholus similis isolates on banana plants (Musa AAA v. Poyo). Nematropica, 25(2), 135-141
FAO (Food and Agriculture Organization of United Nations). (2004). La economía mundial del banano 1985-2002. FAO. Fogain, R. (2000). Effect of Radopholus similis on plant growth and yield of plantains (Musa AAB). Nematology, 2, 129-133.
González, C., Aristizábal, M. y Aristizábal, J. (2007). Dinámica poblacional de nematodos fitopatógenos en plátano (Musa AAB) Dominico Hartón. Agronomía, 15(2), 25-31.
Gowen, S., Quénéhervé, P. y Fogain, R. (2005). Nematode parasites of bananas and plantains. En: M. Luc (Eds), Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (pp. 617-657). CABI publishing.
Guzmán, Ó. y Castaño, J. (2020). Nematodos fitoparásitos en cultivos tropicales. Manual de Diagnóstico. Academia Colombiana de Ciencias Exactas Físicas y Naturales. 281p.Guzmán, Ó. (2011). El nematodo barrenador (Radopholus similis [Cobb] Thorne) del banano y plátano. Luna Azul, 32, 137-153.
Jaraba, J. y Lozano, Z. (2008). Meloidogyne spp. asociados al cultivo de plátano (Musa AAB) en Tierralta y Valencia (Córdoba). Temas Agrarios, 12(2), 36-44.
Jenkins, W. (1964). A rapid centrifugal flotation technique for separating nematodes from soil. Plant Disease Report, 48(9), 692.
Kaur, H. y Attri, R. (2013). Morphological and morphometrical characterization of Meloidogyne incognita from different host plants in four districts of Punjab, India. J. Nematol., 45(2), 122-127.
Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of molecular evolution, 16(2), 111-120.
Koshy, P. K., Jasy, T., Mathew, J. y Nampoothiri, C. K. (1991). Morphometric variability in Radopholus similis. Indian Journal of Nematology, 21(1), 24-38.
Kumar, S., Stecher, G., Li, M., Knyaz, C. y Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547-1549.
Lambert, K. y Bekal, S. (2002). Introduction to plant-parasitic nematodes. The plant Health instructor, 10, 1094-1218.
Larsson, A. (2014). AliView: a fast and lightweight alignment viewer and editor for large data sets. Bioinformatics, 30(22), 3276-3278.
Leigh, J. W. y Bryant, D. (2015). Popart: full-feature software for haplotype network construction. Methods in Ecology and Evolution, 6(9): 1110-1116.
MADR (Ministerio de Agricultura y Desarrollo Rural). (2017). Cifras sectoriales cadena del cultivo de banano. Sistema de Información de Gestión y Desempeño de Organizaciones de Cadena – SIOC. MADR.
Marin, D., Sutton, T. y Barker, K. (1998). Dissemination of bananas in Latin America and the Caribbean and its relationship to the occurrence of Radopholus similis. Plant Disease, 82(9), 964-974.
Meredith, J. (1973). Algunos métodos de campo y laboratorio para trabajar con nematodos. Maracaibo, Venezuela. p.44.
Mgonja, D. M., Temu, G. E., Ndunguru, J. C., Mziray, M. F., Lyantagaye, S. L. y Luambano, N. D. (2020). Molecular approach to confirm traditional identification of sampled in Tanzania. Journal of Nematology, 52(20), 1-8.
Múnera, G. (2008). Biodiversity of phytoparasitic nematodes associated with Musaceae and fruit crops in Colombia (Doctoral dissertation). Ghent University.
Múnera, G., Bert, W. y Decraemer, W. (2009). Morphological and molecular characterisation of Pratylenchus araucensis n. sp. (Pratylenchidae), a root-lesion nematode associated with Musa plants in Colombia. Nematology, 11(6), 799-813.
Múnera, G., Bert, W., Vierstraete, A., De La Peña, E. y Decraemer, W. (2010). Burrowing nematodes from Colombia and their relationship with Radopholus similis populations, R. arabocoffeae and R. duriophilus. Nematology, 12(4), 619-629.
Perry, R. N. y Moens, M. (2013). Plant nematology. Cabi International. UK. 569 pp.
Plowright, R., Dusabe, J., Coyne, D. y Speijer, P. (2013). Analysis of the pathogenic variability and genetic diversity of the plantparasitic nematode Radopholus similis on bananas. Nematology, 15(1), 41-56.
Roy, S., Roy, K., Sarkar, S., Rathod, A. y Hore, J. (2018). Intra-specific morphological and morphometric variability of Radopholus similis (Cobb, 1893) Thorne, 1949. Journal of Applied and Natural Science. 10(3): 841-846.
Rozas, J. P., Librado, J. C., Sánchez, X. y Rozas, R. 2010. DnaSP version 5.10. 1 (Selfextracting file of 4.6 Mb). Universidad de Barcelona.
Ryss, A. Y. y Wouts, W. M. (1997). The genus Radopholus (Nematoda: Pratylenchidae) from native vegetation in New Zealand, with descriptions of two new species. Int. J. Nematol., 7, 1-17.
Ryss, A. Y. (2003). Taxonomy, evolution and phylogeny of the genus Radopholus (didelphic species) according to morphological data, with a key to species (Nematoda: Tylenchida). Zoosystematica Rossica, 11(2002), 243-256.
Sarah, J., Pinochet, J. y Stanton, J. (1996). El nematodo barrenador del banano Radopholus similis Cobb. Plagas de Musa – Hoja Divulgativa No. 1. (Diciembre, 1996). INIBAP.
Sher, S. (1968). Revision of the genus Radopholus Thome, 1949 (Nematoda, Tylenchoidea). Proc. Helminthol. Soc. Wash., 35, 219-237.
Siddiqi, M. (2000). Tylenchida: Parasites of plants and insects. CABI Bookshop. UK. 834 pp.
Sikora, R., Coyne, D. y Quénéhervé, P. (2018). Chapter 17: Nematode parasites of bananas and plantains. Pp. 617-657. In: R. Sikora, D. Coyne, J. Hallmann y P. Timper (Eds.), Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. CABI Publishing.
Tan, M., Cobon, J., Aitken, E. y Cook, L. G. (2010). Support for the ‘out-of-Southeast Asia hypothesis for the origin of Australian populations of Radopholus similis (Cobb, 1893) (Nematoda: Pratylenchidae). Systematic Parasitology, 77(3), 175-183.
Taylor, A. L. (1969). The Fiji banana-root nematode, Radopholus similis. Proc. Helminthol. Soc. Wash., 36, 157-163.
Thorne, G. (1961). Principles of nematology. McGraw-Hill Book Company, USA. 553 p.
Valencia, R., Guzmán, O., Villegas, B. y Castaño. J. (2014). Manejo integrado de nematodos fitoparásitos en almácigos de plátano dominico hartón (Musa AAB Simmonds). Luna Azul, 39, 165-185.
Araya, M. (1995). Efecto depresivo de ataques de Radopholus similis en banano (Musa AAA). Corbana, 20(43), 3-6.
Arboleda, F., Guzmán, A. y Mejía, L. (2012). Efecto de extractos cetónicos de higuerilla sobre el nematodo barrenador en condiciones in vitro. Luna Azul. 35, 28-48.
Bird, A. F. y Bird, J. (2012). The structure of nematodes. Academic Press. Castillo-Russi, J., Araya-Vargas, M. y Patiño-Hoyos, L. (2010). Respuesta a la aplicación de nematicida en banano en la zona de Urabá, Colombia. Agronomía Mesoamericana, 21(2), 307-317.
Castresana, J. (2000). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution, 17(4), 540-552.
Cenibanano. (Centro de Investigaciones del Banano). (2018). Informe Cenibanano 2018.
CIAT (Centro Internacional de Agricultura Tropical). (2013). Producción de material de “siembra” limpio en el manejo de las enfermedades limitantes de plátano. CIAT.
Costa, D., Faleiro, G., Cares, J. y Gomes, A. (2008). Pathogenicity and genetic variability of Radopholus similis populations in bananas (Musa acuminata AAA and AA) based on RAPD analysis. Nematologia Brasileira Piracicaba (SP) Brasil. 32(4), 303-316.
Edgar, R. C. (2004). Muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res., 32(5),1792-1797
Elbadri, G. A., Geraert, E. y Moens, M. (1999). Morphological differences among Radopholus similis (Cobb, 1893) Thorne, 1949 populations. Russian Journal of Nematology, 7(2), 139-153.
Elbadri, A., De Ley, P., Waeyenberge, L., Vierstraete, A., Moens, M. y Vanfleteren, J. (2002). Intraspecific variation in Radopholus similis isolates assessed with restriction fragment length polymorphism and DNA sequencing of the internal transcribed spacer region of the ribosomal RNA cistron. International Journal for Parasitology, 32,199-205.
Fallas, G. A., Sarah, J. L. y Fargette, M. (1995). Reproductive fitness and pathogenicity of eight Radopholus similis isolates on banana plants (Musa AAA v. Poyo). Nematropica, 25(2), 135-141
FAO (Food and Agriculture Organization of United Nations). (2004). La economía mundial del banano 1985-2002. FAO. Fogain, R. (2000). Effect of Radopholus similis on plant growth and yield of plantains (Musa AAB). Nematology, 2, 129-133.
González, C., Aristizábal, M. y Aristizábal, J. (2007). Dinámica poblacional de nematodos fitopatógenos en plátano (Musa AAB) Dominico Hartón. Agronomía, 15(2), 25-31.
Gowen, S., Quénéhervé, P. y Fogain, R. (2005). Nematode parasites of bananas and plantains. En: M. Luc (Eds), Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (pp. 617-657). CABI publishing.
Guzmán, Ó. y Castaño, J. (2020). Nematodos fitoparásitos en cultivos tropicales. Manual de Diagnóstico. Academia Colombiana de Ciencias Exactas Físicas y Naturales. 281p.Guzmán, Ó. (2011). El nematodo barrenador (Radopholus similis [Cobb] Thorne) del banano y plátano. Luna Azul, 32, 137-153.
Jaraba, J. y Lozano, Z. (2008). Meloidogyne spp. asociados al cultivo de plátano (Musa AAB) en Tierralta y Valencia (Córdoba). Temas Agrarios, 12(2), 36-44.
Jenkins, W. (1964). A rapid centrifugal flotation technique for separating nematodes from soil. Plant Disease Report, 48(9), 692.
Kaur, H. y Attri, R. (2013). Morphological and morphometrical characterization of Meloidogyne incognita from different host plants in four districts of Punjab, India. J. Nematol., 45(2), 122-127.
Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of molecular evolution, 16(2), 111-120.
Koshy, P. K., Jasy, T., Mathew, J. y Nampoothiri, C. K. (1991). Morphometric variability in Radopholus similis. Indian Journal of Nematology, 21(1), 24-38.
Kumar, S., Stecher, G., Li, M., Knyaz, C. y Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547-1549.
Lambert, K. y Bekal, S. (2002). Introduction to plant-parasitic nematodes. The plant Health instructor, 10, 1094-1218.
Larsson, A. (2014). AliView: a fast and lightweight alignment viewer and editor for large data sets. Bioinformatics, 30(22), 3276-3278.
Leigh, J. W. y Bryant, D. (2015). Popart: full-feature software for haplotype network construction. Methods in Ecology and Evolution, 6(9): 1110-1116.
MADR (Ministerio de Agricultura y Desarrollo Rural). (2017). Cifras sectoriales cadena del cultivo de banano. Sistema de Información de Gestión y Desempeño de Organizaciones de Cadena – SIOC. MADR.
Marin, D., Sutton, T. y Barker, K. (1998). Dissemination of bananas in Latin America and the Caribbean and its relationship to the occurrence of Radopholus similis. Plant Disease, 82(9), 964-974.
Meredith, J. (1973). Algunos métodos de campo y laboratorio para trabajar con nematodos. Maracaibo, Venezuela. p.44.
Mgonja, D. M., Temu, G. E., Ndunguru, J. C., Mziray, M. F., Lyantagaye, S. L. y Luambano, N. D. (2020). Molecular approach to confirm traditional identification of sampled in Tanzania. Journal of Nematology, 52(20), 1-8.
Múnera, G. (2008). Biodiversity of phytoparasitic nematodes associated with Musaceae and fruit crops in Colombia (Doctoral dissertation). Ghent University.
Múnera, G., Bert, W. y Decraemer, W. (2009). Morphological and molecular characterisation of Pratylenchus araucensis n. sp. (Pratylenchidae), a root-lesion nematode associated with Musa plants in Colombia. Nematology, 11(6), 799-813.
Múnera, G., Bert, W., Vierstraete, A., De La Peña, E. y Decraemer, W. (2010). Burrowing nematodes from Colombia and their relationship with Radopholus similis populations, R. arabocoffeae and R. duriophilus. Nematology, 12(4), 619-629.
Perry, R. N. y Moens, M. (2013). Plant nematology. Cabi International. UK. 569 pp.
Plowright, R., Dusabe, J., Coyne, D. y Speijer, P. (2013). Analysis of the pathogenic variability and genetic diversity of the plantparasitic nematode Radopholus similis on bananas. Nematology, 15(1), 41-56.
Roy, S., Roy, K., Sarkar, S., Rathod, A. y Hore, J. (2018). Intra-specific morphological and morphometric variability of Radopholus similis (Cobb, 1893) Thorne, 1949. Journal of Applied and Natural Science. 10(3): 841-846.
Rozas, J. P., Librado, J. C., Sánchez, X. y Rozas, R. 2010. DnaSP version 5.10. 1 (Selfextracting file of 4.6 Mb). Universidad de Barcelona.
Ryss, A. Y. y Wouts, W. M. (1997). The genus Radopholus (Nematoda: Pratylenchidae) from native vegetation in New Zealand, with descriptions of two new species. Int. J. Nematol., 7, 1-17.
Ryss, A. Y. (2003). Taxonomy, evolution and phylogeny of the genus Radopholus (didelphic species) according to morphological data, with a key to species (Nematoda: Tylenchida). Zoosystematica Rossica, 11(2002), 243-256.
Sarah, J., Pinochet, J. y Stanton, J. (1996). El nematodo barrenador del banano Radopholus similis Cobb. Plagas de Musa – Hoja Divulgativa No. 1. (Diciembre, 1996). INIBAP.
Sher, S. (1968). Revision of the genus Radopholus Thome, 1949 (Nematoda, Tylenchoidea). Proc. Helminthol. Soc. Wash., 35, 219-237.
Siddiqi, M. (2000). Tylenchida: Parasites of plants and insects. CABI Bookshop. UK. 834 pp.
Sikora, R., Coyne, D. y Quénéhervé, P. (2018). Chapter 17: Nematode parasites of bananas and plantains. Pp. 617-657. In: R. Sikora, D. Coyne, J. Hallmann y P. Timper (Eds.), Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. CABI Publishing.
Tan, M., Cobon, J., Aitken, E. y Cook, L. G. (2010). Support for the ‘out-of-Southeast Asia hypothesis for the origin of Australian populations of Radopholus similis (Cobb, 1893) (Nematoda: Pratylenchidae). Systematic Parasitology, 77(3), 175-183.
Taylor, A. L. (1969). The Fiji banana-root nematode, Radopholus similis. Proc. Helminthol. Soc. Wash., 36, 157-163.
Thorne, G. (1961). Principles of nematology. McGraw-Hill Book Company, USA. 553 p.
Valencia, R., Guzmán, O., Villegas, B. y Castaño. J. (2014). Manejo integrado de nematodos fitoparásitos en almácigos de plátano dominico hartón (Musa AAB Simmonds). Luna Azul, 39, 165-185.
