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ABSTRACT: A protocol for the evaluation of acrosomal integrity of fresh boar sperm fixed in 2% glutaraldehyde was adjusted for use in sperm diluted at 3×106 espermatozoa per ml in BSD-Lamons and refrigerated at 17°C. Fourteen sires sampled three times (3 of maternal and 11 of paternal line) were used to evaluate acrosomal integrity in fresh semen (F), at laboratory arrival (0h), and 24, 48, and 72 h later. Acrosomal morphology was classified in the following groups: normal apical ridge (NAR), missing apical ridge (MAR), damaged apical ridge (DAR), and loose acrosomal cap (LAC). The effects of storage time and genetic line were analyzed using ANOVA with a mixed model fore repeated measurements in the same individual. In general two response patterns were observed in acrosomal integrity. Results at F and 0h were not different between them, but were different from the results at 24, 48, and 72 h, which were not different among them. The percentage of NAR was higher (P<0.001) in F and 0h (mean 96.93±0.23%) than at 24, 48, and 72 h (mean 93.72±0.49%), and was higher in the maternal than in the paternal line (95.84±0.32 vs. 94.18±0.22%, respectively; P<0,001). A technique for the evaluation of acrosomal integrity in fresh semen was adapted for evaluation of diluted sperm, indicating that it can be used for seminal evaluations for quality control of commercially available diluted semen.
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