Authors
Abstract
ABSTRACT: With the objective of evaluating the nutritional metabolic balance of selenium in grazing sheep, heparinized blood samples were taken during winter and spring seasons in 2 groups of 7 ewes each (prior to lambing and after lambing) located at the IX Region of Araucania, Chile (38° LS y 72° LW). A compounded NADPH-dependent kinetic method was used to analyse the GSH-Px activity in blood. The mean and standard deviation of the obtained values were calculated, and the “t” test was used to compare the results between groups. The blood activity of GSH-Px was 158 ± 60 U/g Hb, and was similar between groups (P>0,05). Within the group of ewes prior to breeding, 20% exhibited low values (< 60 U/g Hb) of GSH-Px blood activity, while the lactating ewes group didn’t present low values, but 20% did show a marginal value (< 130 U/g Hb), according to the productive cycle of sheep in the IX Region, Chile. These results suggest that there are metabolic imbalances of selenium in grazing sheep in Southern Chile, being the gestating ewes the most affected.
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References
Barquinero, J. Un ejército de radicales libres en acción. In: Crystal, R.G.; Ramon, J.R. Glutatión: eje de la defensa antioxidante. ExcerptaMedicaMedicalCommunications. B.V. Amsterdam, 1992.
Burk, R.F.; Hill, K.E. Regulation of selenoproteins. Annual Reviews Nutrition. v.13, p.65-81, 1993.
Jaramillo, S.; Villa, N.A.; Pineda, A.F.; et al. Blood activity of superoxide dismutase and glutathione peroxidase in grazing dairy heifers. Pesquisa Agropecuária Brasileira. v.40, p.1115-1121, 2005.
Ceballos, A.; Wittwer, F.G. Metabolismo del selenio en rumiantes. Archivos de Medicina Veterinaria. v.28, p.5-18, 1996.
Ceballos, A.; Wittwer, F.; Contreras, P.A. et al. Actividad sanguínea de glutatión peroxidasa en rebaños lecheros a pastoreo: variación según edad y época del año. Archivos de Medicina Veterinaria. v.30, p.13-22, 1998.
Cloete, S.W.P.; Van-Niekerk, F.E.; Kritzinger, N.M. et al. Production responses of sheep supplemented with copper, cobaltand selenium on kikuyu ryegrass pastures. Journal of the South African Veterinary Association. v.65, p.52-58, 1994.
Crempien, C. Deficiencia de selenio en corderos. Agronegocios. p.45-46, 1990.
Graphpad Software. 1996. GraphPad Prism V. 2.01. GraphPad Software, Inc.
Gutteridge, J.M.C.; Halliwell, B. Antioxidants in nutrition, health, and disease. Oxford University Press. Oxford, 1994.
Jain, N.C. Schalm’s Veterinary hematology. 4th ed. Philadelphia: Lea & Febiger, 1986. 1221p.
Hartley, W.J.; Grant, B. A review of selenium responsive diseases of New Zealand livestock. Federation Proceedings. v.20, p.679-688, 1961.
Jarrige, R. Alimentation des bovins, ovins et caprins. INRA. Paris, 1988.
Kolb, E.; Kaskous, S.; Seehawer, J. Ernährungsbiochemische Aspekte der Bedeutung, der Verwertung, des Stoffwechsels und der Andwendung von Vitamin E und von Selen beim Schaf. Berl. Münch. Tierärzt. Wschr. v.110, p.178-184, 1997.
Langlands, J.P.; Donald, G.E.; Bowles, J.E. et al. Subclinical selenium insufficiency. 1. Selenium status and the response in liveweight and wool production of grazing ewes supplemented with selenium. Australian Journal of Experimental Agriculture. v.31, p.25-31, 1991.
Mason, B.D. Nutrition guide for B.C. sheep producers. Ministry of Agriculture and Food, Province of British Columbia. Victoria, 1985.
McDowell, L.R.; Velásquez-Pereira, J.; Valle, G. Minerales para rumiantes en pastoreo en regiones tropicales. University of Florida. Gainesville, 1997.
Miller, J.K.; Brzezinska-Slebodzinska, E.; Madsen, F.C. Oxidative stress, antioxidants, and animal function. Journal Dairy Science. v.76, p.2812-2823, 1993.
Miranda, M.I.; Castillo, C.; Santamarina, G. et al. Evaluación de los niveles de selenio en corderos de raza Gallega. ITEA. v.92a, p.134- 142, 1996.
Rotruck, J.T.; Pope, A.L.; Ganther, H.E. et al. Selenium: Biochemical role as a component of gluthatione peroxidase. SCIENCE. v.179, p.588-590, 1973.
Rouanet, J.L.; Romero, O.; Demanet, R. Áreas agroecológicas en la IX Región: descripción. Investigación Programa Agropecuario Carillanca. v.7, p.18-23, 1988.
Ruprechter, G.; Wittwer, F.; Contreras, P.A. et al. Actividad sanguínea de glutatión peroxidasa en bovinos, equinos, ovinos, llamas y alpacas a pastoreo. Archivos de Medicina Veterinaria. v.30, p.57-58, 1998.
Suttle, N.F.; Jones, D.G.; Woolliams, C. et al. Heinz body anemia in lambs with deficiencies of copper or selenium. The British Journal of Nutrition. v.58, p.539-548, 1987.