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Publication Date: 2008-12-19
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Romero Peñuela, M. H. ., & Sánchez Valencia, J. A. . (2008). Seroprevalence of Trypanosoma cruzi by the Western Blot technique in canine population in the State of Tolima, Colombia. Revista Veterinaria Y Zootecnia (On Line), 2(2), 48–52. Retrieved from https://revistasojs.ucaldas.edu.co/index.php/vetzootec/article/view/5723
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Abstract
ABSTRACT: The Chagas disease is a zoonosis of chronic evolution caused by the protozoan Trypanosoma cruzi, which affects man and canines. It is transmitted mainly by the vectors Triatoma infestans and Rhodnius prolixus, in a domestic transmission cycle in Colombia. A serological survey was carried out with 211 mongrel canines from six municipalities of the department of Tolima. The serum was analyzed by Western blot technique, using specific epitopes named TESA antigens. A seroprevalence of 1.42% (3/211 serums) was obtained in the evaluated group. These results show the low prevalence of anti-Trypanosoma antibodies in these canine populations. The study suggests that canines play a non important role in the transmission of Trypanosoma cruzi in this endemic area in Colombia.
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References
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Umezawa, E.; Bastos, S.; Camargo, M.E.; et al. Evaluation of recombinant antigens for serodiagnosis of Chagas´ disease in South and Central America. Journal of Clinical Microbiology, v.37, p.1554-1560, 1999.
Umezawa, E.; Luquetti, A.; Levitus, G.; et al. Serodiagnosis of chronic and acute Chagas disease with Trypanosoma cruzi recombinant proteins: results of a collaborative study in six Latin American countries. Journal of Clinical Microbiology, v.42, p.449-452, 2004.
Brutus, L.; Shneider, D.; Postigo, J.; et al. Congenital Chagas disease: Diagnostic and clinical aspects in an area without vectorial transmission, Bermejo, Bolivia. Acta Tropica (2008). doi:10.1016/j.actat ropica.2008.03.009. In press.
Caliari, M; Machado, R.; Lana, M.; et al. Quantitative analysis of cardiac lesions in chronic canine chagasic cardiomyopathy. Revista do Instituto Medicina Tropical de São Paulo, v.44, n.5, p.273–278, 2002.
Castanera, M.B.; Lauricella, M.A.; Chuit, R.; et al. Evaluation of dogs as sentinels of the transmission of Trypanosoma cruzi in a rural area of north- western Argentina. Annals of Tropical Medicine & Parasitology, v.92, n.6, p.671–683, 1998.
Corredor, A; Alvarez, C.A.; Agudelo C.A.; et al. Prevalence of Trypanosoma cruzi and Leishmania chagasi infection and risk factors in a Colombian indigenous population. Revista do Instituto Medicina Tropical São Paulo, v.41, n.4, p.229– 234, 1999.
Diosque, P.; Padilla, A.; Cimino, R.; et al. Chagas disease in rural areas of Chaco province Argentina: epidemiologic survey in humans, reservoirs, and vectors. The American Society of Tropical Medicine and Hygiene. v.71, n.5, p.590-593, 2004.
Estrada-Franco, J.G.; Bathia, V.; Díaz-Albiter, H.; et al. Human Trypanosoma cruzi infection and seropositivity in dogs, Mexico. Emergeting Infection Disease. v.21, n.4, p.624- 630, 2006.
Gomes-Silva, A.; Silveira-Lacereda, P.; Pereira, J., et al. Immunoblotting anal ices using two- dimensional gel electrophoresis of Trypanosoma cruzi excreted-secreted antigens. Revista da Sociedades Brasileira de Medicina Tropical. v.37, n.6, p.454- 459, 2004.
Guhl, S. & Nicholls, S. Manual de procedimientos para el diagnóstico de la enfermedad de Chagas. 1.ed. Bogotá, Colombia: Organización Mundial de la Salud, Ministerio de Salud de Colombia, Instituto Nacional de Salud y Universidad de los Andes, 2001. p.98.
Gurtler, R.; Cohen, J.; Cecere, M.; et al. Influence of humans and domestic animals on the household prevalence of Trypanosoma cruzi in Triatoma infestans populations in northwest Argentina. The American Society of Tropical Medicine and Hygiene. v.58, n.6, p.748-758, 1998.
Instituto Colombiano Agustín Codazzi. Distribution and characterization of canine Chagas disease in Texas. Diccionario geográ£co de Colombia. Bogotá D.C., 1996.
Kjos, S.A.; Snowden, T.M.; Craig, T.M.; et al. Distribution and characterization of canine Chagas Disease in Texas. Veterinary Parasitology, v.152, p.249–256, 2008.
Lauricella, M.A.; Castanera, M.B.; Gürtler, R.E.; et al. Immunodiagnosis of Trypanosoma cruzi (Chagas Disease) Infection in Naturally Infected Dogs. Memórias do Instituto Oswaldo Cruz, v.93, n.4, p.501–507, 1998.
Machado, E.; Fernandes, A.; Murta, S.; et al. A study of experimental reinfection by Trypanosoma cruzi in dogs. The American Society of Tropical Medicine and Hygiene, v.65, n.6, p.958–965, 2001.
Montenegro, V.; Jímenez, M.; Pinto Dias, J.C.; et al. Chagas disease in dogs from endemic areas of Costa Rica. Memórias do Instituto Oswaldo Cruz, v.97, n.4, p.491-494, 2002.
Nakazawa, M.; Pereira, D.; Moura M.; et al. Excretory-secretory antigens of Trypanosoma cruzi are potentially useful for serodiagnosis of Chronic Chagas disease. Clinical and Diagnostic Laboratory Immunology, v.8, n.5, p.1024-1027, 2001.
Neto, V.A.; De Marchi, C.; Ferreira, A. Observations on the use of TESA blot for the serological diagnosis of Chagas disease. Revista da Sociedades Brisileira de Medicina Tropical. v.38, n.6, p.534-535, 2005.
Rosypal, A.C.; Cortés-Vecino, J.A. ; Gennari, S.M.; et al. Serological survey of Leishmania infantum and Trypanosoma cruzi en dogs from urban areas of Brazil and Colombia. Veterinary Parasitology, v.149, p.172-177, 2007.
Shadomy, S.; Waring, S.C.; Chappell, C. Combined use of Enzyme-linked Immunoabsorbent assay and flow cytometry to detect antibodies to Trypanosoma cruzi in domestic canines in Texas. Clinical and Diagnostic Laboratory Immulogy, v.3, p.313-319, 2004.
Umezawa, E.; Bastos, S.; Camargo, M.; et al. Evaluation of recombinant antigens for serodiagnosis of Chagas disease in South and Central America. Journal of Clinical Microbiology, v.37, p.1554- 1560, 1996.
Umezawa, E.; Bastos, S.; Camargo, M.E.; et al. Evaluation of recombinant antigens for serodiagnosis of Chagas´ disease in South and Central America. Journal of Clinical Microbiology, v.37, p.1554-1560, 1999.
Umezawa, E.; Luquetti, A.; Levitus, G.; et al. Serodiagnosis of chronic and acute Chagas disease with Trypanosoma cruzi recombinant proteins: results of a collaborative study in six Latin American countries. Journal of Clinical Microbiology, v.42, p.449-452, 2004.
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