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Publication Date: 2009-06-19
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Uribe-Velásquez, L. F. ., Lenz Souza, M. I. ., & Osorio, J. H. (2009). Ultrasound and endocrine evaluation of the ovarian response to a single dose of eCG following a 14-day treatment with CIDR in the breeding season in goats. Revista Veterinaria Y Zootecnia (On Line), 3(1), 38–50. Retrieved from https://revistasojs.ucaldas.edu.co/index.php/vetzootec/article/view/5714
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Abstract
ABSTRACT: The objective of the present study was to compare the effect of different dosage of equine chorionic gonadotrophin (eCG) on the follicular dynamics and hormonal concentrations in Alpine goats, which were subjected to estrous and ovulation synchronization protocols with controlled internal drug released (CIDR) devices containing 0.3 g of progesterone for 14 days during breeding season. Female goats (n = 24) were divided at random into four groups of 6 animals each. On the day of the dispositive removal, the animals received 0 (T1-Control), 200 (T2), 300 (T3) and 400 UI of eCG (T4). Follicular development was observed ultrasonographically starting 1 day prior to eCG administration until the next ovulation. The daily plasma E2 and P4 concentrations were determined using RIA. The continuous variables were evaluated with the variance analyses under GLM procedures of SAS statistical package. The mean comparison was carried out with the Tukey-Kramer test. Estrous response for all treatment groups was 100%. Estrous cycles with three and four waves of follicular growth were observed. The size of the largest follicle was smaller (P<0.01) in control animals of wave 3 (5.5x0.50 mm) when compared with the Treatment 3 at the same wave (7.17x0.35 mm). The effects of eCG on follicular growth showed a significant increase in the number of small and medium follicles (P<0.05) than for the control group. The mean number of corpora lutea were higher (P<0.05) in T4 (400 UI) than T2 (200 UI), showing 4.27x0.23 vs 1.95x0.19 CL, respectively. There were significant differences in plasma concentrations P4 (P<000.01) and E2 (P<0.001) among treatments. The use of CIDR plus intramuscular injection of eCG was effective in inducing estrus, increasing the recruitment of small follicles and accelerating the mechanism of follicular growth in Alpine goats.
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References
Ali, A. Effect of time of eCG administration on follicular response and reproductive performance of FGA-treated Ossimi ewes. Small Ruminant Research, v.72, p.33-37, 2007.
Amorim, E.A.M.; Torres, C.A.A.; Amorim, L.S. et al. Dinâmica follicular em cabras da raça Toggenburg em lactação tratadas ou não com somatotropina bovina recombinante. Arquivos Brasileiros de Medicina Veterinária e Zootecnia, v.59, n.6, p.1500-1508, 2007.
Barret, D.M.W.; Bartlewski, P.M.; Batista-Arteaga, M. et al. Ultrasound and endocrine evaluation of the ovarian response to a single dose of 500 UI of eCG following a 12-day treatment with progestogen-releasing intravaginal sponges in the breeding and nonbreeding season in ewes. Theriogenology, v.61, p.311-327, 2004.
Bevers, M.M.; Dieleman, S.J.; Van Tol, H.T.M. et al. Changes in pulsatile secretion patterns of LH, FSH, progesterone, androstenedione and oestradiol in cows after superovulation with PMSG. Journal of Reproduction and Fertility, v.87, p.745-754, 1989.
Campbell, B.K.; Mann, G.E.; McNeilly, A.S. et al. Pulsatile secretion of inhibin, oestradiol and androstenedione by the ovary of the sheep during the oestrus cycle. Journal of Endocrinology, v.126, p.385-93, 1990.
Castonguay, F.; Dufour, J.J.; Minvielle, F. et al. Follicular dynamics and dominance in Boorola x Finnish Landrace and Boorola x Suffolk ewes heterozygous for the F gene. Journal Reproductin and Fertility, v.89, p.193-203, 1990.
Cline, M.A.; Ralston, J.N.; Seals, C. et al. Intervals from norgestomet withdrawal and injection of equine chorionic gonadotrophin or P.G. 600 to estrus and ovulation in ewes. Journal of Animal Science, v.79, p.589-594, 2001.
Driancourt, M.A.; Fry, R.C. Effect of superovulation with pFSH or PMSG on growth and maturation of the ovulatory follicles in sheep. Animal Reproduction Science, v.27, p.279-292, 1992.
Drion, P.V.; Furtoos, V.; Baril, G. et al. Four years of induction/synchronization of estrus in dairy goats: effect on the evolution of eCG binding rate in relation with the parameters of reproduction. Reproduction, Nutrition and Development, v.41, p.401-412, 2001.
Evans, G.; Robinson, T.J. The control of fertility in sheep: endocrine and ovarian responses to progestagen-PMSG treatment in the breeding season and in anoestrus. Journal of Agricultural Science, v.94, p.69-88, 1980.
Fonseca, J.F.; Torres, C.A.A.; Santos, A.D.F. et al. Progesterone and behavioral features when estrous is induced in Alpine goats. Animal Reproduction Science, v.103, p.366-373, 2008.
Freitas, V.J.F.; Salles, M.G.F. Adaptation of eCG (equine chorionic gonadotrophin dose for estrus synchronization of dairy goats raised in Northeast Brazil: preliminary results. In: International Conference of Goats, 7, 2000, Tours. Proceedings… Institut de L´Élevage and INRA, 2000. p.465-466.
Ginther, O.J.; Kot, K. Follicular dynamics during the ovulatory season in goats. Theriogenology, v.42, p.987-1001, 1994.
Hervé, V.; Roy, F.; Bertin, J. et al. Antiequine chorionic gonadotrophin (eCG) antibodies generated in goats treated with eCG for the induction of ovulation modulate the luteinizing hormone and follicle stimulating hormone bioactivities of eCG differently. Endocrinology, v.145, p.294-303, 2004.
Kouskoura, T.H.; Kouimtzis, S.; Alexaki, E. et al. Comparative studies of ovarian steroids in blood, and specific proteolytic enzymes in the cervical mucus, in four sheep breeds after oestrus synchronization (Progesterone and PMSG). 1. Breed variation of oestradiol-17 þ and progesterone in blood during natural oestrus, synchronization oestrus, and the first oestrus after synchronized oestrus. Reproduction Domestic Animal, v.30, p.8-13, 1995.
Kridli, R.T.; Al-Khetlib, S.S. Reproductive responses in ewes treated with eCG or increasing doses of royal jelly. Animal Reproduction Science, v.92, p.75-85, 2006.
Lafrance, M.; Goff, A.K. Effect of pregnancy on oxytocin-induced release of prostaglandin F2a in heifers. Biology of Reproduction, v.3, p.1113- 1119, 1985.
Latour, D.; Littell, R. Advanced general linear models with an emphasis on mixed models. Version 6.12, Copyright by SAS Institute Inc., Cary, USA, 1996. 268p.
Leyva, V.; Buckrell, B.C.; Walton, J.S. Regulation of follicular and ovulation in ewes by exogenous progestagen. Theriogenology, v.50, p.395-416, 1998.
Liu, X.; Dai, Q.; Hart, E.J. et al. Ultrasonographic characteristics of ovulatory follicles and associated endocrine changes in cyclic ewes treated with medroxyprogesterone acetate (MAP)-releasing intravaginal sponges and equine chorionic gonadotrophin (eCG). Reproduction Domestic Animal, v.42, p.393-401, 2007.
Martínez, J.J.; Izaguirre, F.; Sánchez, L. et al. Comportamiento reproductivo de ovejas Barbados Barriga Negra sincronizadas con MPA y diferentes tiempos de aplicación de eCG durante la época de baja fertilidad. Revista Cientí£ca, v.17, p.47-52, 2007.
McGinley, R.; Casey, J.H. Analysis of progesterone in unextrated serum; a method using danazol (17 a-preg-4-en-20-yno (2,3) isoxasol-17-ol) a bloker of steroid binding to proteins. Steroids, v.33, p.127-138, 1979.
Menchaca, A.; Vilariño, M.; Crispo, M. et al. Day O Protocol: Superstimulatory treatment initiated in the absence of a large follicles improves ovarian response and embryo yield in goats. Theriogenology, v.68, p.1111-1117, 2007.
Mies Filho, A.; Endler, J.O.; Dutra, J. et al. Indução do estro ovulatório em ovelhas com o emprego de estímulos elétricos e/ou hormonais - nota prévia. Revista Brasileira de Reprodução Animal, v.13, p.229-238, 1989.
Moor,R.M.;Osborn,J.C.;Crosby,I.M.Gonadotrophin- induced abnormalities in sheep oocytes after superovulation. Journal Reproduction of Fertility, v.74, p.167-172, 1985.
Nephew, K.P.; Cardenas, H.; McClure, K.E. et al. Effects of administration of human chorionic gonadotrophin or progesterone before maternal recognition of pregnancy on blastocyst development and pregnancy in sheep. Journal of Animal Science, v.47, p.283-294, 1994.
Nishigai, M.; Takamura, A.; Kamomae, H. et al. The effect of human chorionic gonadotrophin on the development and function of bovine corpus luteum. Journal Reproduction Development, v.47, p.283-294, 2002.
Noel, B.; Bister, J.L.; Pierquin, B. et al. Effects of FGA and PMSG on follicular growth and LH secretion in Suffolk ewes. Theriogenology, v.41, p.719-727, 1994.
Pearce, D.T.; Robinson, T.J. Plasma progesterone concentrations, ovarian and endocrinological responses and sperm transport in ewes with synchronized oestrus. Journal of Reproduction and Fertility, v.75, p.49-62, 1985.
Ravindra, J.P.; Rawlings, N.C.; Evans, A.C.O. et al. Ultrasonographic study of ovarian follicular dynamics in ewes during the estrous cycle. Journal of Reproduction and Fertility, v.101, p.501-509, 1994.
Riesenberg, S.; Meinecke-Tillmann, S.; Meinecke,B. Ultrasonic survey of follicular development following superovulation with a single application of pFSH, eCG or hMG in goats. Small Ruminant Research, v.40, p.83-93, 2001.
Ritar, A.J.; Salamon, S.; Ball, P.D. et al. Ovulation and fertility in goats after intravaginal device- PMSG treatment. Small Ruminant Research, v.2, p.323-331, 1989.
Rubianes, E.; Castro, T.; Carbajal, B. et al. Effect of high progesterone levels during the growing phase of the dominant follicle of wave 1 in ultrasonically monitored ewes. Canadian Journal of Animal Science, v.76, p.1-13, 1996.
Scaramuzzi, R.J.; Adams, N.R.; Baird, D.T. et al. A model for follicle selection and the determination of ovulation rate in the ewe. Reproduction Fertility Development, v.5, p.459-478, 1993.
Schrick, F.N.; Surface, R.A.; Pritchard, J.Y. et al. Ovarian structures during the estrous cycle and early pregnancy in ewes. Biology of Reproduction, v.49, p.1133-1140, 1993
Silveira, K.B.X.; Kozicki, L.E. Superovulação em ovelhas Suffolk durante o anestro sazonal com FSH e PMSG. Revista Brasileira de Reproducao Animal, v.25, n.3, 2001.
Thatcher, W.W.; Moreira, F.; Pancarci, S.M. et al. Strategies to optimize reproductive by regulation of ovarian function. Domestic Animal Endocrinology, v.23, p.243-254, 2002.
Tenório Filho, F.; Santos, M.H.B.; Carrazzoni, P.G. et al. Follicular dynamics in Anglo-Nubian goats using transrectal and transvaginal ultrasound. Small Ruminant Research, v.72, p.51-56, 2007. Uribe-Velásquez, L.F.; Oba, E.; Lara-Herrera, L.C. et al. Respostas endócrinas e ovarianas associadas com o folículo dominante da primeira onda folicular em ovelhas sincronizadas com CIDR ou PGF2a. Revista Brasileira de Zootecnia, v.31, p.944-953, 2002.
Uribe-Velásquez, L.F.; Oba, E.; Souza, M.I.L. Población folicular y concentraciones plasmáticas de progesterona (P4) en ovejas sometidas a diferentes protocolos de sincronización. Archivos de Medicina Veterinaria, v.40, p.83-88, 2008a.
Uribe-Velásquez, L.F.; Oba, E.; Souza, M.I.L. Efeitos da progesterona exógena sobre o desenvolvimento folicular em ovelhas. Arquivos Brasileiros de Medicina Veterinária e Zootecnia, v.60, p.58- 65, 2008b.
Amorim, E.A.M.; Torres, C.A.A.; Amorim, L.S. et al. Dinâmica follicular em cabras da raça Toggenburg em lactação tratadas ou não com somatotropina bovina recombinante. Arquivos Brasileiros de Medicina Veterinária e Zootecnia, v.59, n.6, p.1500-1508, 2007.
Barret, D.M.W.; Bartlewski, P.M.; Batista-Arteaga, M. et al. Ultrasound and endocrine evaluation of the ovarian response to a single dose of 500 UI of eCG following a 12-day treatment with progestogen-releasing intravaginal sponges in the breeding and nonbreeding season in ewes. Theriogenology, v.61, p.311-327, 2004.
Bevers, M.M.; Dieleman, S.J.; Van Tol, H.T.M. et al. Changes in pulsatile secretion patterns of LH, FSH, progesterone, androstenedione and oestradiol in cows after superovulation with PMSG. Journal of Reproduction and Fertility, v.87, p.745-754, 1989.
Campbell, B.K.; Mann, G.E.; McNeilly, A.S. et al. Pulsatile secretion of inhibin, oestradiol and androstenedione by the ovary of the sheep during the oestrus cycle. Journal of Endocrinology, v.126, p.385-93, 1990.
Castonguay, F.; Dufour, J.J.; Minvielle, F. et al. Follicular dynamics and dominance in Boorola x Finnish Landrace and Boorola x Suffolk ewes heterozygous for the F gene. Journal Reproductin and Fertility, v.89, p.193-203, 1990.
Cline, M.A.; Ralston, J.N.; Seals, C. et al. Intervals from norgestomet withdrawal and injection of equine chorionic gonadotrophin or P.G. 600 to estrus and ovulation in ewes. Journal of Animal Science, v.79, p.589-594, 2001.
Driancourt, M.A.; Fry, R.C. Effect of superovulation with pFSH or PMSG on growth and maturation of the ovulatory follicles in sheep. Animal Reproduction Science, v.27, p.279-292, 1992.
Drion, P.V.; Furtoos, V.; Baril, G. et al. Four years of induction/synchronization of estrus in dairy goats: effect on the evolution of eCG binding rate in relation with the parameters of reproduction. Reproduction, Nutrition and Development, v.41, p.401-412, 2001.
Evans, G.; Robinson, T.J. The control of fertility in sheep: endocrine and ovarian responses to progestagen-PMSG treatment in the breeding season and in anoestrus. Journal of Agricultural Science, v.94, p.69-88, 1980.
Fonseca, J.F.; Torres, C.A.A.; Santos, A.D.F. et al. Progesterone and behavioral features when estrous is induced in Alpine goats. Animal Reproduction Science, v.103, p.366-373, 2008.
Freitas, V.J.F.; Salles, M.G.F. Adaptation of eCG (equine chorionic gonadotrophin dose for estrus synchronization of dairy goats raised in Northeast Brazil: preliminary results. In: International Conference of Goats, 7, 2000, Tours. Proceedings… Institut de L´Élevage and INRA, 2000. p.465-466.
Ginther, O.J.; Kot, K. Follicular dynamics during the ovulatory season in goats. Theriogenology, v.42, p.987-1001, 1994.
Hervé, V.; Roy, F.; Bertin, J. et al. Antiequine chorionic gonadotrophin (eCG) antibodies generated in goats treated with eCG for the induction of ovulation modulate the luteinizing hormone and follicle stimulating hormone bioactivities of eCG differently. Endocrinology, v.145, p.294-303, 2004.
Kouskoura, T.H.; Kouimtzis, S.; Alexaki, E. et al. Comparative studies of ovarian steroids in blood, and specific proteolytic enzymes in the cervical mucus, in four sheep breeds after oestrus synchronization (Progesterone and PMSG). 1. Breed variation of oestradiol-17 þ and progesterone in blood during natural oestrus, synchronization oestrus, and the first oestrus after synchronized oestrus. Reproduction Domestic Animal, v.30, p.8-13, 1995.
Kridli, R.T.; Al-Khetlib, S.S. Reproductive responses in ewes treated with eCG or increasing doses of royal jelly. Animal Reproduction Science, v.92, p.75-85, 2006.
Lafrance, M.; Goff, A.K. Effect of pregnancy on oxytocin-induced release of prostaglandin F2a in heifers. Biology of Reproduction, v.3, p.1113- 1119, 1985.
Latour, D.; Littell, R. Advanced general linear models with an emphasis on mixed models. Version 6.12, Copyright by SAS Institute Inc., Cary, USA, 1996. 268p.
Leyva, V.; Buckrell, B.C.; Walton, J.S. Regulation of follicular and ovulation in ewes by exogenous progestagen. Theriogenology, v.50, p.395-416, 1998.
Liu, X.; Dai, Q.; Hart, E.J. et al. Ultrasonographic characteristics of ovulatory follicles and associated endocrine changes in cyclic ewes treated with medroxyprogesterone acetate (MAP)-releasing intravaginal sponges and equine chorionic gonadotrophin (eCG). Reproduction Domestic Animal, v.42, p.393-401, 2007.
Martínez, J.J.; Izaguirre, F.; Sánchez, L. et al. Comportamiento reproductivo de ovejas Barbados Barriga Negra sincronizadas con MPA y diferentes tiempos de aplicación de eCG durante la época de baja fertilidad. Revista Cientí£ca, v.17, p.47-52, 2007.
McGinley, R.; Casey, J.H. Analysis of progesterone in unextrated serum; a method using danazol (17 a-preg-4-en-20-yno (2,3) isoxasol-17-ol) a bloker of steroid binding to proteins. Steroids, v.33, p.127-138, 1979.
Menchaca, A.; Vilariño, M.; Crispo, M. et al. Day O Protocol: Superstimulatory treatment initiated in the absence of a large follicles improves ovarian response and embryo yield in goats. Theriogenology, v.68, p.1111-1117, 2007.
Mies Filho, A.; Endler, J.O.; Dutra, J. et al. Indução do estro ovulatório em ovelhas com o emprego de estímulos elétricos e/ou hormonais - nota prévia. Revista Brasileira de Reprodução Animal, v.13, p.229-238, 1989.
Moor,R.M.;Osborn,J.C.;Crosby,I.M.Gonadotrophin- induced abnormalities in sheep oocytes after superovulation. Journal Reproduction of Fertility, v.74, p.167-172, 1985.
Nephew, K.P.; Cardenas, H.; McClure, K.E. et al. Effects of administration of human chorionic gonadotrophin or progesterone before maternal recognition of pregnancy on blastocyst development and pregnancy in sheep. Journal of Animal Science, v.47, p.283-294, 1994.
Nishigai, M.; Takamura, A.; Kamomae, H. et al. The effect of human chorionic gonadotrophin on the development and function of bovine corpus luteum. Journal Reproduction Development, v.47, p.283-294, 2002.
Noel, B.; Bister, J.L.; Pierquin, B. et al. Effects of FGA and PMSG on follicular growth and LH secretion in Suffolk ewes. Theriogenology, v.41, p.719-727, 1994.
Pearce, D.T.; Robinson, T.J. Plasma progesterone concentrations, ovarian and endocrinological responses and sperm transport in ewes with synchronized oestrus. Journal of Reproduction and Fertility, v.75, p.49-62, 1985.
Ravindra, J.P.; Rawlings, N.C.; Evans, A.C.O. et al. Ultrasonographic study of ovarian follicular dynamics in ewes during the estrous cycle. Journal of Reproduction and Fertility, v.101, p.501-509, 1994.
Riesenberg, S.; Meinecke-Tillmann, S.; Meinecke,B. Ultrasonic survey of follicular development following superovulation with a single application of pFSH, eCG or hMG in goats. Small Ruminant Research, v.40, p.83-93, 2001.
Ritar, A.J.; Salamon, S.; Ball, P.D. et al. Ovulation and fertility in goats after intravaginal device- PMSG treatment. Small Ruminant Research, v.2, p.323-331, 1989.
Rubianes, E.; Castro, T.; Carbajal, B. et al. Effect of high progesterone levels during the growing phase of the dominant follicle of wave 1 in ultrasonically monitored ewes. Canadian Journal of Animal Science, v.76, p.1-13, 1996.
Scaramuzzi, R.J.; Adams, N.R.; Baird, D.T. et al. A model for follicle selection and the determination of ovulation rate in the ewe. Reproduction Fertility Development, v.5, p.459-478, 1993.
Schrick, F.N.; Surface, R.A.; Pritchard, J.Y. et al. Ovarian structures during the estrous cycle and early pregnancy in ewes. Biology of Reproduction, v.49, p.1133-1140, 1993
Silveira, K.B.X.; Kozicki, L.E. Superovulação em ovelhas Suffolk durante o anestro sazonal com FSH e PMSG. Revista Brasileira de Reproducao Animal, v.25, n.3, 2001.
Thatcher, W.W.; Moreira, F.; Pancarci, S.M. et al. Strategies to optimize reproductive by regulation of ovarian function. Domestic Animal Endocrinology, v.23, p.243-254, 2002.
Tenório Filho, F.; Santos, M.H.B.; Carrazzoni, P.G. et al. Follicular dynamics in Anglo-Nubian goats using transrectal and transvaginal ultrasound. Small Ruminant Research, v.72, p.51-56, 2007. Uribe-Velásquez, L.F.; Oba, E.; Lara-Herrera, L.C. et al. Respostas endócrinas e ovarianas associadas com o folículo dominante da primeira onda folicular em ovelhas sincronizadas com CIDR ou PGF2a. Revista Brasileira de Zootecnia, v.31, p.944-953, 2002.
Uribe-Velásquez, L.F.; Oba, E.; Souza, M.I.L. Población folicular y concentraciones plasmáticas de progesterona (P4) en ovejas sometidas a diferentes protocolos de sincronización. Archivos de Medicina Veterinaria, v.40, p.83-88, 2008a.
Uribe-Velásquez, L.F.; Oba, E.; Souza, M.I.L. Efeitos da progesterona exógena sobre o desenvolvimento folicular em ovelhas. Arquivos Brasileiros de Medicina Veterinária e Zootecnia, v.60, p.58- 65, 2008b.
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