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ABSTRACT: Introduction: The routine methods used for lipid profile measurement in humans, a species with LDL pattern, needs to be evaluated for its use in the lipid profile determination for species with HDL pattern. Objective: The objective of the present study was to compare the direct method and the precipitation method for the measurement of HDL cholesterol in species with HDL pattern. Materials and Methods: Samples of 200 horses of different ages without discrimination by sex or age that were fasting were obtained. After serum extraction, levels of HDL cholesterol were determined using the direct and the precipitation methods. The results were statistically analyzed using one way ANOVA. Results: The direct method reported values in mg/dl for media, minimal, maximal, range, and standard deviation of 62.2; 37; 101; 64; 10.7 respectively. The precipitation method reported values for media, minimal, maximal, range, and standard deviation of 62,14; 40; 101; 61; 2,8 respectively. The P value in test F is greater or equal to 0.05, reason why there is no statistical significant evidence at a confidence level of 95% between values obtained using both methods. Conclusion: It is recommended to use any of the two analyzed methods for HDL-cholesterol measurement in species with HDL pattern.
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References
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