PDF (Español)
FLIP
Authors
Abstract
ABSTRACT: The objective of the study was to identify neutralizing antibodies against Bovine Herpesvirus 1 in the Colombian Magdalena Medio region. Using the serum neutralization test in 150 animals from different subregions from the Colombian Magdalena Medio region who came for slaughtering in the Central Slaughter House in Dorada, Caldas, the presence of serum antibodies against bovine herpesvirus was analyzed. The serum neutralization test in plaque was performed in the virology laboratory of the Federal University of Santa Maria, Rio Grande do Sul, Brazil. The Cooper 100 TCDI50 of BoHV-1 stump and MDBK cells were used. Eighty animals (53.3%) were positive considering 1 / 2 the cutoff point, while 68 (45.3%) were negative for viral agent. Two samples (1.3%) showed toxicity in cell cultures. Results differing variables such as sex, age, production system and origin of seropositive animals are presented. We discuss the significance of antibodies in bovines. Research should be conducted to develop a risk assessment system and economic losses induced by the presence of the virus, and to establish the critical points and the appropriate time for
vaccination to maintain the balance agent, host, and environment. In addition, the implementation of new regional studies aimed at direct diagnosis of the agent is recommended.
Keywords
References
Büchen-Osmond, C. Alphabetical List of ICTV approved Virus Families and Genera. Sixth Report of the International Committee on Taxonomy of Viruses. Created April, 1995. Última actualización: 8 de abril de 1998. Revisado: octubre de 2010.
Campos, F.S.; Franco, A.C.; Hübner, S.O.; Oliveira, M.T.; Silva, A.D. et al. High prevalence of co-infections with bovine herpesvirus 1 and 5 found in cattle in southern Brazil. Vet Mic, 139, p.67-73, 2009. CIAT. Reportes Anuales. 1972, 1973, 1974, 1975. p.39- 40.
Góngora, A.; Villamil, L.C.; Vera, V.; Ramírez, G.; Parra, J. Diagnóstico de las principales enfermedades reproductivas en toros de la Sabana de Bogotá. Énfasis en RIB. Rev, Med Vet Zoot, 43, p.37-41, 1995.
Jones, C. Herpes Simplex Virus Type 1 and Bovine Herpesvirus 1 Latency. Clin Microbiol Rev, 16, p.79-95, 2003.
Karhs, R.F. Infectious bovine rhinotracheitis a infectious Vulvovaginitis. In: Viral diseases of cattle. 2.ed. Ames: Iowa State University, 2001. p.159-170.
Lemaire, M.; Meyer, G.; Ernest, E.; Wanherreweghe, B.; Limbourg, P.; Pastoret, P.P.; Thiry, E. Latent bovine herpesvirus 1 infection in calves protected by calostral inmunity. The Veterinary Record, 137(3), p.70-1, 1995.
Médici, K.C.; Alfieri, A.A.; Alfieri, A.F. Prevalência de anticorpos neutralizantes contra o herpesvírus bovino tipo 1 decorrente de infecção natural em rebanhos com distúrbios reprodutivos. Ciência Rural, Santa Maria, 30(2), p.347-350, 2000.
Meyer, G.; Lemaire, M.; Ros, C.; Belak, K.; Gabriel, A.; Cassart, D.; Coignoul, F.; Belak, S.; Thiry, E. Comparative pathogenesis of acute and latent infections of calves with bovine herpes virus type 1 and 5. Arch Virol, 146, p.633-652, 2001. Ministerio de la Protección Social. Decreto 1500 de mayo 4 de 2007. p.41.
Molano, D.; Rodríguez, J.L.; Ramírez, G.; Villamil, L.C. Caracterización electroforética e inmunológica de una cepa de campo del virus de la rinotraqueitis infecciosa bovina y su comparación con cepas de referencia. Rev Med Vet Zoot, 44, p.35-8, 1996.
Piedrahita, D., Ramírez, G., Vera, V. Detección y caracterización por métodos moleculares de aislamientos colombianos de Herpesvirus bovino tipo 1. Rev Fac Med Vet Zoot, 52, p.122-127, 2005.
Piedrahita, L.E.; Montoya, L.M.; Pedraza, F.J. Herpes Virus Bovino tipo 1 (BoHV-1) como posible causa de encefalitis en bovinos de la región del Magdalena Medio Colombiano. Estudio serológico y análisis epidemiológico. Rev Colomb Cienc Pec, 23, p.191-198, 2010.
Rissi, D.R.; Rench, R.R.; Flores, E.F.; Kommers, G.D.; Barros, C.S. Meningoencefalitis por herpes bovino-5. Pesq vet Bras, 27(7), p.251-260, 2007.
Rivera, H.G.; Benito, A.Z.; Ramos, O.C.; Manchego, A. Prevalencia de enfermedades de impacto reproductivo en bovinos de la Estación Experimental de Trópico del Centro de
Investigaciones IVITA. Rev. Inves. Vet., Perú, 15(2), p.83-86, 2004.
Ruiz-Sáenz, J.; Jaime, J.; Vera, V.J. Prevalencia serológica y aislamiento del Herpesvirus Bovino-1 (BHV-1) en hatos ganaderos de Antioquia y del Valle del Cauca. Rev Colomb Cienc Pec, 23, p.299-307, 2010.
Roizman, B. et al. The family herpesviridae on update. Arch virol, v.123, p.425-499, 1992.
Roizman, B.; Pellett, P.E. The family herpesviridae: A brief introduction. In: Knipe, D.M.; Howley, P.M. (Eds.). Fields Virology. 4.ed. USA: Lippincott Williams & Wilkins, 2001. p.2381-2397.
Silva, M.S.; Brum, M.C.S.; Loreto, E.L.S.; Weiblen, R.; Flores, E.F. Molecular and antigenic characterization of Brazilian bovine herpesvirus type 1 isolates recovered from the brain of cattle with neurological disease. Virus Res, 129, p.191- 199, 2007.
Takiuchi, E.; Medici, K.C.; Alfieri, A.F. Bovine herpesvirus type 1 abortions detected by a seminested PCR in Brazilian cattle herds. Rev Vet Sci, 79, p.85-88, 2005.
Vera, V.; Betancur, C. Aislamiento del virus herpes bovino tipo 1 en bovinos del departamento de Córdoba, Colombia. Rev MVZ, Córdoba, 13, p.1495-1503, 2008.
Weiblen, R. Doenças víricas que interferem na produção leiteira. In: Charles, T.P.; Furlong, J. (Eds.). Doenças dos Bovinos de Leite Adultos. Coronel Pacheco. EMBRAPA CNPGL, 1992. p.45-62.
Wyler, R.; Engels, M.; Schwyzer, M. Infectious Bovine Rhinotracheitis/ Vulvovaginitis (BHV-1). Institute of Virology, University of Zurich, 1995. p.1-55.
Zapata, J.C.; Ossa, J.E.; Zuluaga, F.N. Actualización de los viejos enigmas y visión de futuro de la RIB en Colombia. Rev Colomb Cienc Pec, 15, p.155- 159, 2002.
