Efeito precoce do concentrado autólogo de plaquetas sobre a osteoartrose induzida em coelhos: Níveis plasmáticos de PDGF e TGF-β1 e seu potencial como biomarcadores

Abstract

O objetivo da presente pesquisa foi determinar a concentração dos fatores de crescimento PDGF e TGF-β1 no plasma de coelhos com osteoartrose induzida pela ruptura do ligamento cruzado cranial e tratados com concentrado autólogo de plaquetas ou Ringer Lactato intra-articular. Foram empregados 14 coelhos machos, adultos, com massa corporal média de 3,0 kg, da raça Nova Zelândia que foram divididos em dois grupos (I e II). Amostras de sangue foram coletadas antes da ruptura do ligamento cruzado cranial (momento 1), no momento da substituição do mesmo (momento 2) e duas semanas após a reparação do ligamento (momento 3) e submetidas a centrifugação para se obter o plasma. A concentração dos fatores de crescimento foi avaliada empregando-se kits de ELISA específicos para a espécie e para os fatores de crescimento. Nos coelhos do grupo concentrado autólogo de plaquetas verificou-se diferença estatisticamente significativa (P<0,05) nos níveis do PDGF no momento 3 quando comparados com o grupo que recebeu a solução de Ringer Lactato intraarticular. Os níveis plasmáticos do TGF-β1 apresentaram diferença estatisticamente significativa (P<0,05) no grupo controle quando comparados o momento 1 com o momento 2 e o momento 3, porém não foi observada diferença significativa quando comparados com o grupo que recebeu o concentrado autólogo de plaquetas em nenhum dos momentos avaliados. Conclui-se, portanto, que o PDGF pode ser usado como parâmetro de avaliação precoce do efeito do concentrado autólogo de plaquetas intraarticular.

Keywords:

Cranial cruciate ligament

degenerative articular process

growth factors

plasma

regenerative therapy

fatores de crescimento

ligamento cruzado cranial

plasma

processo articular degenerativo

terapia regenerativa

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