Dynamic response of decrease in Salmonella spp. in the cecum of chickens: Impact of using mananooligosaccharides and β-glucans (1,3) (1,6)

Abstract

Salmonella spp. is classified as a Gram-negative bacterium and is characterized as a facultative anaerobe. The worldwide prevalence of Salmonella enteritidis is approximately 100 million cases. Aim: To study the effect of mananooligosaccharides and β-glucans (1,3) (1,6) for stimulating cellular immune response at the gastrointestinal level in the cecum of chickens to reduce Salmonella spp. colonization. Methods: A systematic review and meta-analysis were performed, including studies performed between 1980 and 2020. A total of 16 research papers with 28 treatments were chosen, and the statistical software Stat Graphics Stratus was used. Commands comparing the test with multiple ranges were used; groups treated with β-glucans (1,3) (1,6) in the cecum were used for determining the prevalence of Salmonella enteritidis, and groups not treated with β-glucans were used as control. The significance (P > 0.05) was validated by Tukey’s test and the corresponding linear regression equation was obtained. Results: There were differences between the prevalence of cecum-contaminated chickens in the β-glucan (1,3) (1,6) treated group and the control group. A mean of 29.60 ± 4.84 SD was observed in the treated group vs. 62.52 ± 47.6 SD in the control group (P = 0.0000), based on which it can be concluded that there is a highly significant difference in favor of the group treated with β-glucans (1,3) (1,6). A statistical contrast test was performed over the differences, which yielded a result in favor of using β-glucans (1,3) (1,6) because they decrease Salmonella spp. contamination of the cecum by 32.93 ± 13.3%. Conclusions: βGlucans (1,3) (1,6) can be used to reduce Salmonella spp. colonization in the cecum of chickens, which would consequently decrease the systemic transfer of this bacterium to other organs, especially to eggs that are for human consumption.

Keywords

β-glucans

mananooligosaccharides

Salmonella spp

References

Barrow, P.A. 1999. Virulence of Salmonella enterica serovar Enteritidis. In: Saeed, A.M. (ed.). Salmonella enterica serovar Enteritidis in humans and animals. Iowa State University Press, Ames, USA, p. 173-181.

Berg, R.D. 1995. Bacterial translocation from the gastrointestinal tract. Trends Microbiol. 3:149-154.

Desmidt, M., Ducatelle, R., and Haesebrouck, F. 1997. Pathogenesis of Salmonella enteritidis phage type four after experimental infection of young chickens. Vet. Microbiol. 56:99-109.

Ducoing, A.M. 2016. Diseño estadístico de experimentos. Estadística para veterinarios y zootecnistas. Newton. pp. 207.

Farrar, A.M. 2009. The application of research synthesis methods for evaluating primary research on Salmonella in broiler chickens. M.Sc. Thesis. University of Guelph, Canada.

Gast, R.K., and Beard, C.W. 1990. Production of Salmonella enteritidis-contaminated eggs by experimentally infected hens. Avian Dis. 34:438-446.

Howard, Z.R., Moore, R.W., Zabala-Diaz, I.B., Kim, W.K., Birkhold, S.G., Byrd, J.A., Kubena, L.F., Nisbet, D.J., and Ricke, S.C. 2006. In vitro survival and growth of Salmonella typhimurium inoculated on yolk membrane after long term refrigerated storage of shell eggs. J. Con. Prot. Food Saf. 1:30-34.

Humphrey, T.J. 1990. Growth of Salmonellas in intact shell eggs: influence of storage temperature. Vet. Rec. 126:292.

Immerseel, V.F. 2002. Early protection against Salmonella Infection by modification of the initial host–pathogen interactions. World Poutry Sci. J. 58:501-513.

Khan-Mohammed, Z., Adesiyun, A.A., Swanston, W.H., and Chadee, D.D. 2005. Frequency and characteristics of selected enteropathogens in fecal and rectal specimens from childhood diarrhea in Trinidad: 1998-2000. Rev. Panam. salud publ. 17:170-177.

Lowry, V.K., Farnell, M.B., Ferro, P.J., Swaggerty, C.L., Bahl, A., and Kogut, M.H. 2005. Purified beta-glucan as an abiotic feed additive up-regulates the innate immune response in immature chickens against Salmonella enterica serovar Enteritidis. Int. J. Food Microbiol. 98:309-318.

Majowicz, S.E., Musto, J., Scallan, E., Angulo, F.J., Kirk, M., O’Brien, S.J., Jones, T.F., Fazil, A., and Hoekstra, R.M. 2010. The global burden of nontyphoidal Salmonella gastroenteritis. Clin. Infect. Dis. 50:882-889.

Manners, D.J., Masson, A.J., Patterson, J.C., Björndal, H., and Lindberg, B. 1973. The structure of a β-(1→6)-d-glucan from yeast cell walls. Biochem. J. 135:31-36.

Mishu, B., Griffin, P.M., Tauxe, R.V., Cameron, D.N., Hutcheson, R.H., and Schaffner, W. 1991. Salmonella enteritidis gastroenteritis transmitted by intact chicken eggs. Ann. Intern. Med. 115:190-194.

Poppe, C. 2000. Salmonella infections in the domestic fowl. In: Wray, C. and Wray, A. Salmonella in domestic animals. CA B International, New York, US, p. 107-132.

Remus, A., Hauschild, L., Andretta, I., Kipper, M., Lehnen, C.R., and Sakomura, N.K. 2014. A meta-analysis of the feed intake and growth performance of broiler chickens challenged by bacteria. Poultry Science 93:1149-1158.

Rojas, M.W., Anaya, C., Cano, L.H., Ariztizabal, B.B., Gomez, L.M., and Lopera, H.D. 2017. Mucosa del tracto digestivo. Inmunol. Rojas, v 10:218.

Salazar, J.J. 2019. Review. Respuesta del sistema inmune de las aves a él estimulo con βglucanos 1.3/1.6. Veterinaria y Zootecnia 13. DOI: 10.17151/vetzo.2019.13.1.6.

Salazar, J.J., Urrea, G., and Ramirez, J.D. 2019. Meta análisis: efecto immunomodulador sobre la IgA, generado por los β-glucanos 1,3/1,6 (Saccharomyces cerevisiae), en Pollos de engorde, desafiados con Salmonella entérica, Veterinaria y Zootecnia. 13. DOI: 10.17151/vetzo.2019.13.1.5.

Saleh, M.A.D., Amorim, A.B., Grecco, H.A.T., Berto, D.A., Padovani, C.R., Orsi, R.D.O., and Tse, M.L.P. 2015. Int. J. Biol. Macromol. 80:659-667.

Shao, Y., Guo, Y., and Wang, Z. 2013. Β-1,3/1,6-Glucan alleviated intestinal mucosal barrier impairment of broiler chickens challenged with Salmonella enterica serovar Typhimurium. Poult. Sci. 92:1764-1773.

Shivaprasad, H.L. 2000. Fowl typhoid and pullorun disease. Rev. Sci. Tech. OIE 19:405- 424.

Telzak, E.E., Budnick, L.D., Greenberg, M.S.Z., Blum, S., Shayegani, M., Benson, C.E., and Schultz, S. 1990. A nosocomial outbreak of Salmonella enteritidis infection due to the consumption of raw eggs. N. Engl. J. Med. 323:394-397.

Timoney, J.F. 1989. Egg transmission after infection of hens with Salmonella enteritidis phage type 4. Vet. Rec. 125:600-601.

Wallis, T.S. and Galyov, E.E. 2000. Molecular basis of Salmonella-induced enteritis. MicroReview. Mol. Microbiol. 36:997-1005.