Seroprevalence of Brucella canis and Leptospira spp. in canines in the city of Medellín, Colombia

Abstract

The agents that cause diseases of zoonotic importance in canines, such as Canine brucellosis and Leptospirosis, have gained importance in human clinical practice. Objective: To discover the prevalence and behavior of both diseases in the canine population in the city of Medellín to develop measures of prevention and control in this area. Methods: A total of 1,300 canines were sampled to test for Brucella canis and Leptospira spp. using the PARP-2ME and MAT techniques, respectively, to establish the statistical significance of the different variables analyzed (P ≤ 0.05; OR ≥ 1; 95% CI). Results: Seroprevalence was determined to be 7.32% for B. canis and 9.08%, for Leptospira spp. with a 0.77% co-infection rate of both diseases. The most prevalent serovars for Leptospira spp. were Canicola (3.38%), Icterohaemorrhagiae (2.62%), and Pomona (0.92%). A statistical association was reported for B. canis with the commune variable (San Javier P = 0.002; OR = 2.724 / Guayabal P = 0.000; OR = 3.862 / Belén P = 0.002; OR = 2.953), and for Leptospira spp. with the commune variable (Buenos Aires P = 0.011; OR = 2.220) and age (37-48 months P = 0.005; OR = 4.272). Conclusions: This study shows that both agents are in circulation among the canine population in the city and in all the communes analyzed, representing a possible risk of infection to owners and other animals entering into contact with them.

Keywords:

brucelosis

leptospirosis

cleptospirosis

prevalencia

zoonosis

brucellosis

leptospirosis

dogs

prevalence

zoonosis

References

Agudelo, P.; Castro, B.; Rojo, R. et al. Seroprevalencia y factores de riesgo para brucelosis canina en perros domésticos de once comunas de la ciudad de MedellínColombia. Revista de Salud Pública,v.14, n.4, p.644-656, 2012.

Álvarez, L.; Calderón, A.; Rodríguez, V. et al. Seroprevalencia de leptospirosis canina en una comunidad rural del municipio de Ciénaga de Oro, Córdoba (Colombia). Revista U.D.C.A Actualidad & Divulgación Científica,v.14, n.2, p.75-81, 2011.

Ardoino, S.M.; Baruta, D.A.; Toso, R.E. Brucelosis canina. Ciencia veterinaria,v.8, n.1, p.50-61, 2017.

Carreño, L.A.; Salas, D.; Beltrán, K.B. Prevalencia de leptospirosis en Colombia: revisión sistemática de literatura. Revista de Salud Pública,v.19, n.2, p.204-209, 2017.

Castrillón, L.; Giraldo, C.A.; Sánchez, M.M. et al. Factores asociados con la seropositividad a Brucella canis en criaderos caninos de dos regiones de Antioquia, Colombia. Cadernos de Saúde Pública,v.29, n.10, p.1975-1987, 2013.

Castrillón, L.; López, L.; Sancheze, R. et al. Prevalence of presentation of some zoonotic agents transmitted by canines and felines in Medellín, Colombia. Revista Mvz Cordoba,v.24, n.1, p.7119-7126, 2019.

Delaude, A.; Rodriguez, S.; Dreyfus, A. et al. Canine leptospirosis in Switzerland—a prospective cross-sectional study examining seroprevalence, risk factors and urinary shedding of pathogenic leptospires. Preventive veterinary medicine,v.141, p.48-60, 2017.

Echeverri, L.M.; Penagos, S.; Castañeda, L. et al. Características sociodemográficas y clínicas de pacientes con infección por Leptospira spp. atendidos en cuatro centros hospitalarios de Medellín, Colombia, 2008-2013. Biomédica,v.37, n.1, p.62-67, 2017.

Flores, B.J.; Pérezz, T.; Fuertes, H. et al. A cross-sectional epidemiological study of domestic animals related to human leptospirosis cases in Nicaragua. Acta tropica,v.170, p.79- 84, 2017.

Giraldo, C.A.; Ruiz, Z.T.; Olivera, M. Brucella canis en Medellín (Colombia), un problema actual. Revista U.D.C.A Actualidad & Divulgación Científica,v.12, n.1, p.51-57, 2009.

Langoni, H.; Ponte, M.C.d.; Barbosa, D. et al. PESQUISA DE ANTICORPOS E DE DNA DE Leptospira spp. EM SORO CANINO. Veterinária e Zootecnia,v.22, n.3, p.429- 436, 2015.

Lucero, N.E.; Maldonado, P.L.; Kaufman, S. et al. Brucella canis causing infection in an HIV-infected patient. Vector-Borne and Zoonotic Diseases,v.10, n.5, p.527-529, 2010.

Manias, V.; Nagel, A.; Mollerach, A. et al. Endocarditis por Brucella canis: primer caso documentado en un paciente adulto en Argentina. Revista argentina de microbiología,v.45, n.1, p.50-53, 2013.

Meeyam, T.; Tablerk, P.; Petchanok, B. et al. Seroprevalence and risk factors associated with leptospirosis in dogs. Southeast Asian journal of tropical medicine and public health,v.37, n.1, p.148, 2006.

Miotto, B.A.; Guilloux, A.G.A.; Tozzi, B.F. et al. Prospective study of canine leptospirosis in shelter and stray dog populations: Identification of chronic carriers and different Leptospira species infecting dogs. PloS one,v.13, n.7, 2018.

Olivera, M.; Di-Lorenzo, C. Aislamiento de Brucella canis en un humano conviviente con caninos infectados. Informe de un caso. Colombia Médica,v.40, n.2, p.218-220, 2009.

Olivera, M.; Giraldo, C.; Di-Lorenzo, C. Identificación por PCR de Brucella canis en sangre y leche canina: Reporte de un caso. Archivos de medicina veterinaria,v.43, n.3, p.295-298, 2011.

Pulido, A.; Carreño, G.; Mercado, M. et al. Situación epidemiológica de la leptospirosis humana en Centroamérica, Suramérica y el Caribe. Universitas Scientiarum,v.19, n.3, p.247-264, 2014.

Ruíz, J.D.; Giraldo, C.A.; López, L.V. et al. Brucella canis seroprevalence in stray dogs from" Centro de Bienestar Animal" La Perla", Medellín (Colombia), 2008. Revista Colombiana de Ciencias Pecuarias,v.23, n.2, p.166-172, 2010.

Salas. INFORME DE EVENTO LEPTOSPIROSIS, COLOMBIA, 2017. Equipo Funcional Enfermedades Trasmitidas por Vectores. Colombia: INS y MiniSalud, 2018.

Sánchez, M.; Giraldo, C.A.; Olivera, M. Infección por Brucella canis en humanos: propuesta de un modelo teórico de infección a través de la ruta oral. Infectio,v.17, n.4, p.193-200, 2013.

Sánchez, M.; Ortiz, L.F.; Castrillón, L.L. et al. Application of a polymerase chain reaction test for the detection of Brucella canis from clinical samples of canines and humans. Revista Colombiana de Ciencias Pecuarias,v.27, n.1, p.3-11, 2014.

Souza, T.D.; Carvalho, T.F.; Silva, J.P. et al. Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates. Scientific reports,v.8, n.1, p.1-10, 2018.

Ward, M.P.; Guptill, L.F.; Wu, C.C. Evaluation of environmental risk factors for leptospirosis in dogs: 36 cases (1997–2002). Journal of the American Veterinary Medical Association,v.225, n.1, p.72-77, 2004.