DOI: 10.17151/vetzo.2018.12.1.4
Cómo citar
Osorio-Carmona E., Castro-Varón J. A., Sánchez-Árcila D. F., Sacchetto-Tôrres R. C., CarvalhoNepomuceno A., Carvalho-de Souza J., & de Faria-Rezende C. M. (2018). Early clinical, radiographic and ultrasonographic evaluation of induced osteoarthritis in rabbits treated with autologous platelet concentrate. Revista Veterinaria y Zootecnia (On Line), 12(1), 35 - 61.


Esteban Osorio-Carmona
Universidade Federal de Minas Gerais
Jessica Alejandra Castro-Varón
Universidade Federal de Minas Gerais
Diego Felipe Sánchez-Árcila
Universidade Federal de Minas Gerais
Renato Cesar Sacchetto-Tôrres
Universidade Federal de Minas Gerais
Anelise CarvalhoNepomuceno
Universidade Federal de Minas Gerais
Julia Carvalho-de Souza
Universidade Federal de Minas Gerais
Cleuza Maria de Faria-Rezende
Universidade Federal de Minas Gerais


The objective of this study was to evaluate, the early effect of the autologous platelet concentrates (APC) on induced osteoarthritis (OA) in the stifle joint through clinical, radiographic and ultrasonographic exams. Fourteen adult males of the New Zealand breed rabbits, with an average body mass of 3.0 kg were used to induce OA in the right hind limb (RHL). Rabbits were randomly divided into two groups, control group and APC group. Animals in the control group received 0.5 ml intra-articular Ringer Lactate solution. Immediately after the repair of the cranial cruciate ligament, the APC group received 0.5 ml APC intra-articular injections whose mean concentration of platelets was1.8 times the basal levels. Intra-articular injections were applied immediately after joint stabilization. Rabbits treated with APC showed significantly better clinical scores (p<0.05) when compared with rabbits in the control group in relation to pain sensitivity, joint effusion presence and limb support 15 days after APC application (M3). The range of limb motion showed statistically significant difference (p<0.05) in flexion and extension of RHL in M3 in both groups. The radiographic and ultrassonographic images showed the presence of degenerative joint process in both groups without statistical difference between them. From the results it can be concluded that APC has a beneficial clinical effect in joints affected by OA, favoring the use of the limb and controlling pain sensitivity, and that radiography and ultrasonography images are successful tools in the evaluation of the progression of OA.

 Ayral, X. et al. Synovitis: A potential predictive factor of structural progression of medial tibiofemoral knee osteoarthritis. Results of a 1 year longitudinal arthroscopic study in 422 patients. Osteoarthr Cartilage, v. 13, p. 361-367, 2005.

 Baines, S. Histopathological changes in synovial membrane in cranial cruciate ligament rupture. In: BSAVA Congress, n. 1, 1994, Birmingham.

 Beckwee, D. et al. Osteoarthritis of the knee: Why does exercise work? A qualitative study of the literature. Ageing Res Rev, v. 12, n. 1, p. 226-236, 2013.

 Bolon, B. et al. Interleukin-1beta and tumor necrosis factor alpha produce distinct, time-dependent patterns of acute arthritis in the rat knee. Vet Pathol, v. 41, n. 3, p. 235- 243, 2004.

 Brandt, K.D. Pain, synovitis, and articular cartilage changes in osteoarthritis. Semin Arthritis Rheum, v. 18, n. 4, supl. 2, p. 77-80, 1989.

 Carter, S.D.; BARNES, A.; GILMORE, W.H. Canine rheumatoid arthritis and inflammatory cytokines. Vet Immunol Immunopathol, v. 69, p. 201-214, 1999.

 Clark, T.P. The clinical pharmacology of ciclooxigenase-2-selective and dual inhibitors. VetClin North Am Small Anim Pract, v. 36, n. 5, p. 1061-1085, 2006.

 de Bruin, T. et al. Interleukin-8 mRNA expression in synovial fluid of canine stifle joints with osteoarthritis. Vet Immunol and Immunopathol, v. 108, n. 3-4, p. 387-397, 2005.

 Doom, M. et al. Immunopathological mechanisms in dogs with rupture of the cranial cruciate ligament. Vet Immunol Immunopathol, v. 125, n. 1-2, p. 143-161, 2008.

 Flynn, K.A.; Whitcomb, M.B. Equine meniscal injuries: A retrospective study of 14 horses. Proc Am Assoc Equine Pract, v. 48, p. 249-254, 2002.

 Franklin, S.P.; Park, R.D.; Egger, E.L. Metacarpophalangeal and metatarsophalangealosteoarthritis in 49 dogs. J Am Anim Hosp Assoc, v. 45, n. 3, p. 112-117, 2009.

 Froum, S.J. et al. Effect of platelet-rich plasma on bone growth and osseointegration in human maxillary sinus grafts: Three bilateral case reports. Int J Periodontics Restorative Dent, v. 22, n. 1, p. 45-54, 2002.

 Gale, N.W.; Yancopoulos, G.D. Growth factors acting via endothelial cell-specific receptor tyrosine kinases: VEGFs, angiopoietins, and ephrins in vascular development. Genes Dev, v. 13, n. 9, p. 1055-1066, 1999.

 Gao, J.; Symons, A.L.; Bartold, M.P. Expression of transforming growth factor β receptors types II and III within various cells in the rat periodontium. J Periodont Res, v. 34, n. 2, p. 113-122, 1999.

 Gardner, D.L. Problems and paradigms in joint pathology. J Anat, v. 184, Pt. 3, 1994.

 Gnudi, G.; Bertoni, G. Echografic examination of the stifle joint affected by cranial cruciate ligament rupture in the dog. Vet Radiol Ultrasound, v. 42, n. 3, p. 266-270, 2001.

 Guermazi, A. et al. Osteoarthritis: A review of strengths and weaknesses of different imaging options. Rheum Dis Clin N Am, v. 39, n. 3, p. 567-591, 2013.

 Guilak, F. et al. Mechanical and biochemical changes in the superficial zone of articular cartilage in canine experimental osteoarthritis. J Orthop Res, v. 12, n. 4, p. 474-484, 1994.

 Heldin, C.H.; Westermark, B. Mechanism of action and in vivo role of platelet-derived growth factor. Physiol Rev, v. 79, n. 4, p. 1283-1316, 1999.

 Henrotin, Y. et al. Usefulness of specific OA biomarkers, Coll2-1 and Coll2-1NO2, in the anterior cruciate ligament OA canine model.Osteoarthritis Cartilage, v. 20, n. 7, p. 787-790, 2012.

 Huang, T.F. et al. Isolation and characterization of mesenchymal stromal cells from human anterior cruciate ligament. Cytotherapy, v. 10, n. 8, p. 806-814, 2008.

 Iacopetti, I. et al. Application of Platelet-Rich Gel to Enhance Wound Healing in the Horse: A Case Report. J Equine Vet Sci, v. 32, n. 3, p. 123-128, 2012.

 Innes, J.F. et al. Long-term outcome of surgery for dogs with cranial cruciate ligament deficiency. Vet Rec, v. 147, n. 12, p. 325-328, 2000.

 Innes, J.F.; Costello, M.; Barr, M.J. Radiographic progression of osteoarthritis of the canine stifle joint: A prospective study. Vet Rad Ultrasound, v. 45, n. 2, p. 143-148, 2004.

 Johnson, J.M.; Johnson, A.L. Cranial cruciate ligament rupture: Pathogenesis, diagnosis and postoperative rehabilitation. Vet Clin N Am: Small Anim Pract, v. 23, n. 4, p. 717-733, 1993.

 Johnston, S. Osteoarthritis. Vet Clin N Am: Small Anim Pract, v. 27, n. 4, 1997.

 Konttinen, Y.T. et al. Osteoarthritis as an autoinflammatory disease caused by chondrocyte-mediated inflammatory responses. Arthritis Rheum, v. 64, n. 3, p. 613- 616, 2012.

 Lavigne, P. et al. Subchondral and trabecular bone metabolism regulation in canine experimental knee osteoarthritis. OsteoArthritis Cartilage, v. 13, n. 4, p. 310-317, 2005.

 Louboutin, H. et al. Osteoarthritis in patients with anterior cruciate ligament rupture: A review of risk factors. Knee, v. 16, n. 4, p. 239-244, 2009.

 Liu, W.; Burton-Wurster, N.; Glant, T.T. Spontaneous and experimental osteoarthritis in dog: Similarities and differences in proteoglycan levels. J Orthop Res, v. 21, n. 4, p. 730-737, 2003.

 MaCwilliams, P.S.; Friedrichs, K.R. Laboratory evaluation and interpretation of synovial fluid. Vet Clin North Am Small Anim, v. 33, n. 1, p. 153-178, 2003.

 Mercuri, L.G. Osteoarthritis, osteoarthrosis, and idiopathic condylar resorption. Oral Maxillofacial Surg Clin N Am, v. 20, n. 2, p. 169-183, 2008.

 Milano, G. et al. The effect of platelet rich plasma combined with microfractures on the treatment of chondral defects: An experimental study in a sheep model. Osteoarthritis Cartilage, v. 18, n. 7, p. 971-980, 2010.

 Muzzi, L.A.L. et al. Ultra-sonografia da articulação femoro-tibio-patelar em caes normais. Cienc Animal, v. 11, p. 209, 2001.

 Muzzi, L.A.L.; Rezende, C.M.F.; Muzzi, R.A.L. Fisioterapia apos substituição artroscopica do ligamento cruzado cranial em caes. I - avaliacao clinica, radiografica e ultrassonografica. Arq Bras Med Vet Zootec, v. 61, n. 4, p. 805-814, 2009.

 Oliveira, R.R. et al. Radiografia e ultrassonografia no diagnostico da ruptura do ligamento cruzado cranial em caes. Pesq Vet Bras, v. 29, n. 8, p. 661-665, 2009.

 Patel, S. et al. Treatment with Platelet-Rich plasma is more effective than Placebo for knee Osteoarthritis: A Prospective, Double-Blind, Randomized Trial. Am J Sports Med, v. 41, n. 2, p. 356-364, 2013.

 Pelisson, C.F.; TRAPP, S.M.; KEMPER, B. Estudo comparativo in vitro do movimento de gaveta em joelhos de caninos submetidos a duas tecnicas extracapsulares de correcao da instabilidade apos ruptura do ligamento cruzado cranial. Cienc Rural, v. 40, n. 6, p. 1335-1340, 2010.

 Petrigliano, F.P.; McAllister, D.R.; Wu, B.M. Tissue engineering for anterior cruciate ligament reconstruction: A review of current strategies. J Arthr Rel Surg, v. 22, n. 4, p. 441-451, 2006.

 Rayward, R.M.; Thomson, D.G.; Davies, J.V. Progression of osteoarthritis following TPLO surgery: A prospective radiographic study of 40 dogs. J Small Anim Pract, v. 45, p. 92-97, 2004.

 Rezende, C.M.F. et al. Intra-articular autologous platelet concentrate (APC) in the treatment of induced knee osteoarthritis (OA) in rabbits – An arthroscopic evaluation. Intech. Disponível em: Link.

 Ribeiro, A.S.C. O uso de artroscopia no diagnóstico e tratamento da displasia do cotovelo canino. Lisboa, Portugal: Universidade Tecnica de Lisboa, 2011. Dissertacao (Mestrado integrado em Medicina Veterinaria).

 Rychel, J.K. Diagnosis and treatment of osteoarthritis. Top Companion Anim Med, v. 25, n. 1, p. 20-25, 2010.

 Saito, M. et al. Intraarticular administration of platelet-rich plasma with biodegradable gelatin hydrogel microspheres prevents osteoarthritis progression in the rabbit knee. Clin Exp Rheumatol, v. 27, n. 2, p. 201-207, 2009.

 Sanz, S.C.; Bruges-Armas, J.; Beaumont-Herrero, G. Importancia do osso subcondral e da membrana sinovial na patogenia e no tratamento da osteoartrose. Acta Reum Port, v. 31, p. 205-213, 2006.

 Schawalder, P.; Gitterle, E. Eigene methoden zur operativen rekonstruktion bei rupturen des vorderen und hinteren kreuzbandes.Kleintierpraxis, v. 7, p. 323-330, 1989.

 Schnappauf, A. et al. Ultrasound of the canine stifle and its importance in cranial cruciate ligament rupture in dogs. Vet Radiol Ultrasound, v. 42, n. 4, p. 359-394, 2001.

 Schwartz, A.; Martinez-Sanchez, G.; Re, L. Growth factors derived from platelet and its applications in regenerative medicine. Potential use of ozone as activator. Rev Esp Ozonoter, v. 1, n. 1, p. 54-73, 2011.  Silva, R.F. Caracterizações celular e ultra-estrutural do concentrado de plaquetas em cães e gatos e avaliação do seu efeito na osteoartrose em cães. Belo Horizonte, Minas Gerais: Universidade Federal de Minas Gerais, 2012. Tese (Doutorado em Ciencia Animal).

 Tashman, S. et al. Kinematics of the ACL-deficient canine knee during gait: Serial changes over two years. J Orthop Res, v. 22, n. 5, p. 931-941, 2004.

 Tatarunas, A.C.; Matera, J.M.; Franchini, M.L. Analise de liquido sinovial em caes: Revisao de literatura. Rev Educ Contin CRMV-SP, v. 7, n. 1, p. 7-14, 2004.

 Vidotto, V.T. et al. Estudo comparativo do uso de meloxicam por via intra-articular e sistemica no controle da osteoartrite experimentalmente induzida em joelho de coelhos. Rev Bras Ortop, v. 48, n. 6, p. 524-531, 2013.

 Werner, S.; Grose, R. Regulation of wound healing by growth factors and cytokines. Physiol Rev, v. 83, n. 3, p. 835-870, 2003.

 Zhu, Y. et al. Basic science and clinical application of platelet-rich plasma for cartilage defects and osteoarthritis: A review. Osteoarthritis Cartilage, v. 21, n. 1, p. 1627- 1637, 2013.


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