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Publication Date: 2011-07-01
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Moreno López, D., Henao Bustamante, B. E., López Gartner, G. A., Castaño Villa, G. J., & Rivera Páez, F. A. (2011). Sex determination in birds of Furnaridae family by molecular techniques. Boletín Científico. Centro De Museos, 15(2), 130–138. Retrieved from https://revistasojs.ucaldas.edu.co/index.php/boletincientifico/article/view/4614
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Abstract
The Furnariidae family is one of the most diverse groups of birds in the Neotropics, and it is considered as a vulnerable to habitat disturbance group. The sexing of individuals in this family is complex due the lack of clear sexual dimorphism which limits the research in population dynamics and evolutionary studies. In this study, the DNA was extracted from the quill of the feathers using the phenol-chloroform-isoamyl alcohol method in 15 furnarids species captured in four localities from the Department of Caldas between August 2009 and March 2010. The PCR amplification of CHD-Z and CHD-W genes with primers P2-P8 and 2550F-2718R, allowed the differentiation of males from females of seven species (Synallaxis azarae, S. brachyura, Xenops minutus, Dendrocincla tyrannina, Lepidocolaptes lacrymiger, Campylorhampus trochilirostris, C. pusillus), from which there was not previous report of molecular sexing. The efficiency of the source for DNA extraction (80.65%) from feathers as a non-invasive, easy to use, handle and storage method that can be used in population dynamics, genetic structure, reproductive systems and behavioral studies, and in a wider way, in the management and conservation of this family was demonstrated.
References
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ARANGO-B., L., MONTES-R., J.M., LÓPEZ-P., D.A. & LÓPEZ-P., J.O., 2007. Mariposas (Lepidoptera: Papilionoidea, Hesperoidea), escarabajos coprófagos (Coleoptera: Scarabaeinae) y hormigas (Hymenoptera: Formicidae) del Ecoparque Alcázares-Arenillo (Manizales, Caldas – Colombia). Bol. Cient. Mus. Hist. Nat. U. de Caldas, 11(1): 390-409.
BELLO, N., FRANCINO, O. & SÁNCHEZ, A., 2001. Isolation of genomic DNA from feathers. Journal of Veterinary diagnostic investigation, 13: 162-164.
CAMPOS, F., GUTIÉRREZ, F., LÓPEZ, J. & HERNÁNDEZ, M., 2005. Un nuevo criterio para sexar Mirlos Acuáticos Cinclus cinclus en la Península Ibérica. Revista Catalana de Ornitología, 21: 43-46.
CAPARROZ, R., ROBADO, N., BIANCHI, C. & WAYNTAL, A., 2001. Analysis of the genetic variability and breeding behaviour of wild populations of two macaw species (Psittaciformes, Aves) by DNA fingerprinting. Ararajuba, 9 (1): 43-49.
CARDONI, D.J., MALDONADO, J. & ISACCJ, R.G., 2009. Subtle sexual dimorphism in the bay-capped wrenspinetail (Spartonoica maluroides; Furnariidae) uncovered through molecular sex determination. Ornitologia Neotropical, 20: 347-355.
CASTAÑO, G.J., 2004. Evaluación del riesgo de extinción de aves en hábitats fragmentados a largo plazo mediante el análisis de sus características ecológicas: Tesis Maestría, Universidad Nacional de Colombia, Sede Medellín.
CASTAÑO, G.J. & PATIÑO, J.C., 2007. Composición de la comunidad de aves en bosques fragmentados en la región de Santa Elena. Bol. Cient. Mus. Hist. Nat. U. de Caldas, 11: 47-60.
________., 2008. Extinciones locales de aves en fragmentos de bosque en la región de Santa Elena, Andes Centrales, Colombia. El Hornero, 23 (1): 23-34.
CONSTANTINI, V., GUARICCI, A.C., LARICCHIUTA, P., RAUSA, F. & LACALANDRA, G.M., 2008. DNA sexing in Humboldt Penguins (Spheniscus humboldti) from feather samples. Animal Reproduction Science, 106: 162-167.
DE VOLO, S.B., REYNOLDS, R., DOUGLAS, M.R. & ANTOLIN, M.F., 2008. An improved extraction method to increase DNA yield from molted feathers. The Condor, 110 (4): 762-767.
DONOHUE, K. & DUFTY, A., 2006. Sex determination of Red-tailed Hawks (Buteo jamaicensis calurus) using DNA analysis and morphometrics. Journal of Field Ornithology, 77 (1): 74-79.
DOUBLE, M. & OLSEN, P., 1997. Simplified polymerase chain reaction (PCR)-based sexing assists conservation of an endangered owl, the Norfolk Island Boobook Ninox novaeseelandiae undulate. Bird Conservation International, 7: 283-286.
FARIA, L., CARRARA, L. & RODRÍGUEZ, M., 2007. Dimorfismo sexual de tamaño no fura-barreira Hylocryptus rectirostris (Wied) (Aves, Furnariidae). Revista Brasileira de Zoología, 24 (1): 207-212.
FJELDSÅ, J. & KRABBE, N., 1990. Birds of the high Andes. Apollo Books y Zoological Museum, Svendborg y Copenhagen.
FRIDOLFSSON, A.K. & ELLEGREN, H., 1999. A simple and universal method for molecular sexing of nonratite birds. Journal of Avian Biology, 30: 116-121.
GALLEGO, J., 2000. Guía de especies cultivadas en el Jardín Botánico Universidad de Caldas.
GEBHARDT, K.J. & WAITS, L.P., 2008. High error rates for avian molecular sex identification primer sets applied to molted feathers. Journal of Field Ornithology, 79 (3): 286-292.
GRANT, A., 2001. DNA sexing of brown kiwi (Apteryx mantelli) from feather samples. DOC Science Internal Series, 13: 5-16.
GRIFFITHS, R., DOUBLE, M., ORR, K. & DAWSON, R., 1998. A DNA test to sex most birds. Molecular Ecology, 7: 1071-1075.
GRIFFITHS, R. & KORN, R., 1997. A CHD1 gene Z chromosome linked in the chicken (Gallus domesticus). Gene, 197: 225-229.
HARVEY, M.G., BOTER, D.N., STENZLER, L.M. & LOVETTE, I.J., 2006. A comparison of plucked feathers versus blood samples as DNA sources for molecular sexing. Journal of Field Ornithology, 77 (2): 136-140.
HEIMERDINGER, M., 1995. Body pterylosis of woodcreepers and ovenbirds (Dendrocolaptidae and Furnariidae). The Auk, 112 (3): 800-804.
JENSEN, T.F., PERNASETTI, F. & DURRANT, B., 2003. Conditions for rapid sex determination in 47 avian species by PCR of genomic DNA from blood, shell-membrane blood vessels, and feathers. Zoo Biology, 22: 561-571.
KATTAN, G., ÁLVAREZ-LÓPEZ, H. & GIRALDO, M., 1994. Forest fragmentation and bird extinctions: San Antonio eighty years later. Conservation Biology, 8: 138-146.
LIZA, J., MATURRANO, L. & ROSADIO, R., 2008. Determinación del sexo por ADN en cinco especies de guacamayos. Revista de Investigaciones veterinarias de Perú, 19 (1): 31-36.
MATTA, N., RAMÍREZ, N., ZÚÑIGA, B. & VERA, V., 2008. Determinación de sexo en aves mediante herramientas moleculares. Acta Biológica Colombiana, 13 (3): 27-40.
MEZQUIDA, E., 2001. La reproducción de algunas especies de Dendrocolaptidae y Furnariidae en el desierto del monte central, Argentina. Hornero, 16 (1): 23-30.
MORENO, J., MERINO, S., LOBATO, E., RODRÍGUEZ, M. & VÁSQUEZ, R., 2007. Sexual dimorphism and parental roles in the thorn-tailed rayadito (Furnariidae). The Condor, 109: 313-120.
PALMA, L., MIRA, S., CARDIA, P., BEJA, P., GUILLEMAUD, T., FERRAND, N., CANCELA, M.L. & CANCELA DA FONSECA, L., 2001. Sexing Bonelli’s Eagle nestlings: morphometrics versus molecular techniques. Journal of Raptor Research, 35 (3): 187-193.
REMSEN, J.V., 2003. Family Furnariidae (Ovenbirds): 162-357 (en) DEL HOYO, J., ELLIOT, A., CHRISTIE, D.A. (eds.) Handbook of the Birds of the World. Vol. 8. Broadbills to Tapaculos. Lynx Ediciones, Barcelona.
REMSEN, J.V.JR., CADENA, C.D., JARAMILLO, A., NORES, M., PACHECO, J.F., PÉREZ-EMÁN, J., ROBBINS, M.B., STILES, F.G., STOTZ, D.F. & ZIMMER, K.J., 2011. A classification of the bird species of South America. American Ornithologists’ Union. Disponible en: http://www.museum.lsu.edu/~Remsen/SACCBaseline.html
RENJIFO, L.M., 1999. Composition changes in a subandean avifauna after long-term forest fragmentation. Conservation Biology, 13: 1124-1139.
RENJIFO, L.M., FRANCO-MAYA, A.M., AMAYA-ESPINEL, J.D., KATTAN, G.H. & LÓPEZ-LANÚS, B. (eds.)., 2002. Libro rojo de aves de Colombia. Serie Libros Rojos de Especies Amenazadas de Colombia. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt y Ministerio del Medio Ambiente. Bogotá, Colombia. 554p.
RIDGELY, R.S. & TUDOR, G., 1994. The birds of South America. Vol. 2. University of Texas Press, Austin.
ROPER, J.J. & HUTSON, A., 2003. Ovenbirds: 438-441 (en) PERRIS, C.M. (ed.) The new encyclopedia of birds. Oxford University Press, Oxford.
RYDER, T. & DUARAES, R., 2005. It’s not easy being green: using molt and morphological criteria to age and sex greenplumage Manakins (Aves: Pipridae). Ornitologia Neotropical, 16: 481-491.
SALAZAR-RAMÍREZ, L., 2010. Estratificación vertical de aves frugívoras y nectarívoras en plantaciones de aliso y bosque secundario: Trabajo de grado, Universidad de Caldas, Manizales, Caldas, Colombia. 34p.
SANGUINETTI, C.J., DIAS NETO, E. & SIMPSON, A.J., 1994. Rapid silver straining and recovery of PCR products separated on polyacrylamide gels. Biotechniques, 17: 915-919.
SILVEIRA, P., BASTOS, E., MANNAN, R.W. & GUEDES-PINTO, H., 2009. Polymerase chain reaction-single strand conformation polymorphism applied to sex identification of Accipiter cooperii. Molecular and Cellular Probes, 23: 115-118.
SKUTCH, A., 1969. Families Cotingidae, Pipridae, Formicaridae, Furnariidae, Dendrocolaptidae and Picidae. Life histories of Central American Birds III. Berkeley, California. 580p.
WATSON, H.K., MOGG, R.J. & BOND, J.M., 2004. Sexing Eurasian Oystercatchers Haematopus ostralegus from breast feathers collected when ringing. Wader Study Group Bulletin, 105: 87-89.
WILLIS, E.O., 1972. The behavior of plain-brown woodcreepers, Dendrocincla fuliginosa. The Wilson Bulletin, 84 (4): 377-420.
________., 1991. The Ovenbirds: 162-163 (en) FORSHAW, Joseph (ed.) Encyclopedia of Animals: Birds. Merehurst Press, London.
WINKER, K., VOELKER, G.A. & KLICKA, J.T., 1994. A morphometric examination of sexual dimorphism in the Hylophilus, Xenops, and an Automolus from southern Veracruz, Mexico. Journal of Field Ornithology, 65 (3): 307-323.
ARANGO-B., L., MONTES-R., J.M., LÓPEZ-P., D.A. & LÓPEZ-P., J.O., 2007. Mariposas (Lepidoptera: Papilionoidea, Hesperoidea), escarabajos coprófagos (Coleoptera: Scarabaeinae) y hormigas (Hymenoptera: Formicidae) del Ecoparque Alcázares-Arenillo (Manizales, Caldas – Colombia). Bol. Cient. Mus. Hist. Nat. U. de Caldas, 11(1): 390-409.
BELLO, N., FRANCINO, O. & SÁNCHEZ, A., 2001. Isolation of genomic DNA from feathers. Journal of Veterinary diagnostic investigation, 13: 162-164.
CAMPOS, F., GUTIÉRREZ, F., LÓPEZ, J. & HERNÁNDEZ, M., 2005. Un nuevo criterio para sexar Mirlos Acuáticos Cinclus cinclus en la Península Ibérica. Revista Catalana de Ornitología, 21: 43-46.
CAPARROZ, R., ROBADO, N., BIANCHI, C. & WAYNTAL, A., 2001. Analysis of the genetic variability and breeding behaviour of wild populations of two macaw species (Psittaciformes, Aves) by DNA fingerprinting. Ararajuba, 9 (1): 43-49.
CARDONI, D.J., MALDONADO, J. & ISACCJ, R.G., 2009. Subtle sexual dimorphism in the bay-capped wrenspinetail (Spartonoica maluroides; Furnariidae) uncovered through molecular sex determination. Ornitologia Neotropical, 20: 347-355.
CASTAÑO, G.J., 2004. Evaluación del riesgo de extinción de aves en hábitats fragmentados a largo plazo mediante el análisis de sus características ecológicas: Tesis Maestría, Universidad Nacional de Colombia, Sede Medellín.
CASTAÑO, G.J. & PATIÑO, J.C., 2007. Composición de la comunidad de aves en bosques fragmentados en la región de Santa Elena. Bol. Cient. Mus. Hist. Nat. U. de Caldas, 11: 47-60.
________., 2008. Extinciones locales de aves en fragmentos de bosque en la región de Santa Elena, Andes Centrales, Colombia. El Hornero, 23 (1): 23-34.
CONSTANTINI, V., GUARICCI, A.C., LARICCHIUTA, P., RAUSA, F. & LACALANDRA, G.M., 2008. DNA sexing in Humboldt Penguins (Spheniscus humboldti) from feather samples. Animal Reproduction Science, 106: 162-167.
DE VOLO, S.B., REYNOLDS, R., DOUGLAS, M.R. & ANTOLIN, M.F., 2008. An improved extraction method to increase DNA yield from molted feathers. The Condor, 110 (4): 762-767.
DONOHUE, K. & DUFTY, A., 2006. Sex determination of Red-tailed Hawks (Buteo jamaicensis calurus) using DNA analysis and morphometrics. Journal of Field Ornithology, 77 (1): 74-79.
DOUBLE, M. & OLSEN, P., 1997. Simplified polymerase chain reaction (PCR)-based sexing assists conservation of an endangered owl, the Norfolk Island Boobook Ninox novaeseelandiae undulate. Bird Conservation International, 7: 283-286.
FARIA, L., CARRARA, L. & RODRÍGUEZ, M., 2007. Dimorfismo sexual de tamaño no fura-barreira Hylocryptus rectirostris (Wied) (Aves, Furnariidae). Revista Brasileira de Zoología, 24 (1): 207-212.
FJELDSÅ, J. & KRABBE, N., 1990. Birds of the high Andes. Apollo Books y Zoological Museum, Svendborg y Copenhagen.
FRIDOLFSSON, A.K. & ELLEGREN, H., 1999. A simple and universal method for molecular sexing of nonratite birds. Journal of Avian Biology, 30: 116-121.
GALLEGO, J., 2000. Guía de especies cultivadas en el Jardín Botánico Universidad de Caldas.
GEBHARDT, K.J. & WAITS, L.P., 2008. High error rates for avian molecular sex identification primer sets applied to molted feathers. Journal of Field Ornithology, 79 (3): 286-292.
GRANT, A., 2001. DNA sexing of brown kiwi (Apteryx mantelli) from feather samples. DOC Science Internal Series, 13: 5-16.
GRIFFITHS, R., DOUBLE, M., ORR, K. & DAWSON, R., 1998. A DNA test to sex most birds. Molecular Ecology, 7: 1071-1075.
GRIFFITHS, R. & KORN, R., 1997. A CHD1 gene Z chromosome linked in the chicken (Gallus domesticus). Gene, 197: 225-229.
HARVEY, M.G., BOTER, D.N., STENZLER, L.M. & LOVETTE, I.J., 2006. A comparison of plucked feathers versus blood samples as DNA sources for molecular sexing. Journal of Field Ornithology, 77 (2): 136-140.
HEIMERDINGER, M., 1995. Body pterylosis of woodcreepers and ovenbirds (Dendrocolaptidae and Furnariidae). The Auk, 112 (3): 800-804.
JENSEN, T.F., PERNASETTI, F. & DURRANT, B., 2003. Conditions for rapid sex determination in 47 avian species by PCR of genomic DNA from blood, shell-membrane blood vessels, and feathers. Zoo Biology, 22: 561-571.
KATTAN, G., ÁLVAREZ-LÓPEZ, H. & GIRALDO, M., 1994. Forest fragmentation and bird extinctions: San Antonio eighty years later. Conservation Biology, 8: 138-146.
LIZA, J., MATURRANO, L. & ROSADIO, R., 2008. Determinación del sexo por ADN en cinco especies de guacamayos. Revista de Investigaciones veterinarias de Perú, 19 (1): 31-36.
MATTA, N., RAMÍREZ, N., ZÚÑIGA, B. & VERA, V., 2008. Determinación de sexo en aves mediante herramientas moleculares. Acta Biológica Colombiana, 13 (3): 27-40.
MEZQUIDA, E., 2001. La reproducción de algunas especies de Dendrocolaptidae y Furnariidae en el desierto del monte central, Argentina. Hornero, 16 (1): 23-30.
MORENO, J., MERINO, S., LOBATO, E., RODRÍGUEZ, M. & VÁSQUEZ, R., 2007. Sexual dimorphism and parental roles in the thorn-tailed rayadito (Furnariidae). The Condor, 109: 313-120.
PALMA, L., MIRA, S., CARDIA, P., BEJA, P., GUILLEMAUD, T., FERRAND, N., CANCELA, M.L. & CANCELA DA FONSECA, L., 2001. Sexing Bonelli’s Eagle nestlings: morphometrics versus molecular techniques. Journal of Raptor Research, 35 (3): 187-193.
REMSEN, J.V., 2003. Family Furnariidae (Ovenbirds): 162-357 (en) DEL HOYO, J., ELLIOT, A., CHRISTIE, D.A. (eds.) Handbook of the Birds of the World. Vol. 8. Broadbills to Tapaculos. Lynx Ediciones, Barcelona.
REMSEN, J.V.JR., CADENA, C.D., JARAMILLO, A., NORES, M., PACHECO, J.F., PÉREZ-EMÁN, J., ROBBINS, M.B., STILES, F.G., STOTZ, D.F. & ZIMMER, K.J., 2011. A classification of the bird species of South America. American Ornithologists’ Union. Disponible en: http://www.museum.lsu.edu/~Remsen/SACCBaseline.html
RENJIFO, L.M., 1999. Composition changes in a subandean avifauna after long-term forest fragmentation. Conservation Biology, 13: 1124-1139.
RENJIFO, L.M., FRANCO-MAYA, A.M., AMAYA-ESPINEL, J.D., KATTAN, G.H. & LÓPEZ-LANÚS, B. (eds.)., 2002. Libro rojo de aves de Colombia. Serie Libros Rojos de Especies Amenazadas de Colombia. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt y Ministerio del Medio Ambiente. Bogotá, Colombia. 554p.
RIDGELY, R.S. & TUDOR, G., 1994. The birds of South America. Vol. 2. University of Texas Press, Austin.
ROPER, J.J. & HUTSON, A., 2003. Ovenbirds: 438-441 (en) PERRIS, C.M. (ed.) The new encyclopedia of birds. Oxford University Press, Oxford.
RYDER, T. & DUARAES, R., 2005. It’s not easy being green: using molt and morphological criteria to age and sex greenplumage Manakins (Aves: Pipridae). Ornitologia Neotropical, 16: 481-491.
SALAZAR-RAMÍREZ, L., 2010. Estratificación vertical de aves frugívoras y nectarívoras en plantaciones de aliso y bosque secundario: Trabajo de grado, Universidad de Caldas, Manizales, Caldas, Colombia. 34p.
SANGUINETTI, C.J., DIAS NETO, E. & SIMPSON, A.J., 1994. Rapid silver straining and recovery of PCR products separated on polyacrylamide gels. Biotechniques, 17: 915-919.
SILVEIRA, P., BASTOS, E., MANNAN, R.W. & GUEDES-PINTO, H., 2009. Polymerase chain reaction-single strand conformation polymorphism applied to sex identification of Accipiter cooperii. Molecular and Cellular Probes, 23: 115-118.
SKUTCH, A., 1969. Families Cotingidae, Pipridae, Formicaridae, Furnariidae, Dendrocolaptidae and Picidae. Life histories of Central American Birds III. Berkeley, California. 580p.
WATSON, H.K., MOGG, R.J. & BOND, J.M., 2004. Sexing Eurasian Oystercatchers Haematopus ostralegus from breast feathers collected when ringing. Wader Study Group Bulletin, 105: 87-89.
WILLIS, E.O., 1972. The behavior of plain-brown woodcreepers, Dendrocincla fuliginosa. The Wilson Bulletin, 84 (4): 377-420.
________., 1991. The Ovenbirds: 162-163 (en) FORSHAW, Joseph (ed.) Encyclopedia of Animals: Birds. Merehurst Press, London.
WINKER, K., VOELKER, G.A. & KLICKA, J.T., 1994. A morphometric examination of sexual dimorphism in the Hylophilus, Xenops, and an Automolus from southern Veracruz, Mexico. Journal of Field Ornithology, 65 (3): 307-323.
