Authors
Abstract
The karyotypes of three monkeys from the genus Aotus were studied by using banding techniques, including G, C and Q bands. The blood samples for cellular culture were taken from individuals kept in captivity at the "Centro de Rehabilitación de Fauna Silvestre del Oriente de Caldas (CRFSOC), La Victoria (Caldas)" where the animals were being kept temporarily after being confiscated from illegal species traffickers. The blood samples were processed at the Universidad Nacional de Colombia's Cytogenetics Laboratory in Bogotá. Two karyological configurations were found: 2n=52 XX; NF=72, 20 Bi, 32 A. and 2n=53 XX; NF=72, 20+1XX Bi, 30 A. The last one presented a simple robertsonian centric-fusion of chromosomes 13 and 14. The chromosomes were classified into three groups according to size and morphology: A metacentric, B submetacentric, and C acrocentric. It was concluded that the cytogenetically analyzed individuals belonged to the "gray necked" group identified as Aotus griseimembra, a species distributed throughout certain areas in the middle Magdalena river valley, Colombia.
References
ARRIGHI, F. E., HSU, T. C., 1971. Distribution of constitutive heterochromatin in mammalian chromosomes Chromosoma (Berl.). Vol 34. pp. 243-253.
BARRAGÁN, K., 2005. Citogenética en Cérvidos, con énfasis en Venado de Cola Blanca (Odocoileus virginianus). Revista de la Asociación de Veterinarios de Vida Silvestre. 1, (1): 3-9.
BENIRSHCKE, K., KUMAMOTO, A., 1991. Mammalian cytogenetics and conservation of species. The Journal of Heredity. 82: 187-191.
BRUMBACK, R. A., 1973. Two Distinctive Types of Owl Monkeys (Aotus). J. Med. Primatol. 2: 284- 289.
BRUMBACK, R. A., 1974. A third species of the owl monkey Aotus. Journal of Heredity 65:321-323.
BRUMBACK, R. A.; STATON, R. D; BENJAMIN, S.A., LANG, C. M., 1971. The Chromosomes of Aotus trivirgatus Humboldt 1812. Folia Primatol 15: 264- 273.
BUENO, M. L., 2003. Importancia de la caracterización genética de especies silvestres en Zoológicos, Unidades de Rescate de fauna y Centros de acopio. Lyonia .3, (1): 45-55.
CASPERSSON, T.; FABER, S.; FOLEY, G. E.; KUDYNOWSKI, J.; MODEST, E.J.; SIMONSSON, E.; WAGH, U.; ZECH, L., 1968. Chemical Differentiation Along Metaphase Chromosomes. Experimental Cell Research. 49 (1): 219-22.
CASTRILLON, H. J., 2009. Caracterización cariotípica del genero Aotus (Primates: Aotidae) depositados en el Centro de Atención y Valoración de Fauna Silvestre CAV, Departamento del Quindío Colombia. Trabajo de Grado, Universidad del Quindío pp. 23.
DEFLER, T. R., 2003. Primates de Colombia. Conservación Internacional. Serie de guías tropicales de campo. J. V. Rodríguez-Maecha, (ed.) Conservación Internacional, Bogotá, D. C., Colombia. 543.
DEFLER, T. R., BUENO, M. L., 2007. Aotus Diversity and Species Problem. Primate Conservation. 22:55-70
DEFLER, T. R., BUENO, M. L., HERNÁNDEZ-CAMACHO, J., 2001. Taxonomic Status of Aotus hershkovitzi: Its Relationship to Aotus lemurinus lemurinus. Neotropical Primates 9 (2): 37- 52.
DEFLER, T. R., & M. L., BUENO., 2003. Karyological guidelines for Aotus taxonomy. [abstract] Twenty Sixth Annual American Society of Primatology Conference, Calgary, Canada American Journal of Primatology. 60: Suppl.1:134-135.
DESCAILLEAUX, J.; FUJITA, R.; RODRÍGUEZ, L.A.; AQUINO, R.; ENCARNACION, F., 1990. Rearreglos Cromosómicos y Variabilidad Cariotípica del género Aotus (Cebidae: Platyrrhini). La Primatología en el Perú. En: Invetigaciones Primatológicas (1973–1985), Proyecto Peruano de Primatologia "Manuel Moro Sommo" (ed.), pp. 572–577.
GALBREATH, G. J., 1983. Karyotypic evolution in Aotus. American Journal of Primatology. 4: 245-251.
GIRALDO, A.; BUENO, M. L.; SILVA, E.; RAMÍREZ, J.; UMAÑA, J.; ESPINAL, C. 1986. Estudio Citogenético de 288 Aotus Colombianos. Biomedica. 6 (1- 2).
HERSHKOVITZ, P., 1983. Two New Species of Night Monkeys, Genus Aotus (Cebidae: Platyrrhini): A Preliminary Report on Aotus Taxonomy. American Journal of Primatology. 4: 209-243.
LACY, R. C., 1995. What is a Population (and Habitat) Viability Analysis?. Primate Population Viability Analysis Symposium. XVth Congress of The International Primatological Society, Bali, Indonesia, August 1994. Primate Conservation 1993-1994 (14-15): 27-33.
LEVAN, A.; FREDGA, K. & SANDBERG, A., 1964. Nomenclature for Centromeric Position on Chromosomes. Hereditas 52: 201-220.
LORENZO, M. C. & ROJAS, V. R., 2002. Diversidad de Pinnípedos, un enfoque cromosómico. Ciencia y. Desarrollo 26: 62-69.
MA, N. S. F., 1981. Chromosome evolution in the owl monkey. American Journal of Physiology and Anthropolgy, 54: 294 –304.
MA, N. S. F.; JONES, T. C.; MILLER, A. C.; MORGAN, L. M.; ADAMS, E. A., 1976. Chromosome Polyorphism and Banding Patterns in the Owl Monkey (Aotus). Laboratory Animal Science. 26 (6): 1022-1036 Part II of Two Parts.
MA, N. S. F.; ROSSAN, R. N.; KELLEY, S. T.; HARPER, S. J; BEDARD, M. T.; JONES, T. C., 1978. Banding patterns of the chromosomes of two new karyotypes of the owl monkey Aotus captures in Panamá. J Med Primat; (7): 146-147.
Manual de Procedimientos de Laboratorio de Citogenética del Instituto de Genética de la Universidad Nacional de Colombia, Bogotá (MPLC-IGUN). 2009.
MOORHEAD, R.S.; HOWELL, P.C.; MELLMAN, W.J.; BATTEPS, D.M. & HUNDGERFORD, D.A., 1960. Chromosomes preparations of leukocytes cultured from human peripheral blood. Experimental Cellular Research, 2: 613-616.
NAGAMACHI, C. Y.; RODRIGUEZ, L. R.; GALETTI, Jr. P. M.; MANTOVANI, M.; PISSINATI, A.; RISSINO, J. D.; BARROS, R. M. S. & PIECZARKA, J. C., 2003. Cytogenetic studies in Callicebus personatus nigrifrons (Platyrrini, Primates). Caryologia. 56: 47-52.
ORTIZ, M. L.; RODRÍGUEZ, P. A.; BUENO, M. L., 2005. Caracterización Citogenética de la Tortuga Sabanera Podocnemis vogli (reptilia: testudinata: podocnemididae). Acta Biólogica Colombiana. 10 (1): 19-33.
PIECZARKA, J. C & NAGAMACHI, C. Y., 1988. Cytogenetic studies of Aotus from Eastern Amazonia: Y/ Autosome rearrangement. American Journal of Primatology. 14: 255-263.
RODRÍGUEZ-MAECHA, J. V.; ALBERICO, M.; TRUJILLO, F., & JORGENSON, J. (Eds.) 2006. Libro Rojo de los Mamíferos de Colombia. Serie Libros Rojos de Especies Amenazadas de Colombia. Conservación Internacional Colombia y Ministerio de Ambiente, Vivienda y Desarrollo, Bogotá, Colombia, pp. 433
SEABRIGHT, M., 1971. A rapid banding technique for human chromosomes. Lancet 2: 971.
SUMNER, A. T., 1972. A simple technique for demostrating centromeric heterochromatin. Experimental Cell Research . Vol. 75: 304-305.
TEJEDOR M, F., 2001. Aotus y los Atelinae: Nuevas evidencias en la sistemática de los primates platirrinitos. Mastozoología Neotropical. 8 (1): 41-57.
TORRES, O. P.; ENCISO, S.; RUIZ, F.; SILVA, E.; IVAN, Y., 1998. Chromosome diversity of the genus Aotus from Colombia. American journal of primatology. 44: 255-275.
YUNIS, E.; TORRES, O. M. de C.; RAMÍREZ, C., 1977. Genus Aotus: Q and G-band karyotypes and natural hybrids. Folia Primatológica. 27: 165-177.