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Abstract
In order to characterize the dynamics of Sus scrofa (order Artiodactyla, family Suidae) semen quality in the Western-central region of Colombia, information of 1,017 assessments from 177 males from 12 swine farms located between 700 and 2,600 meters above sea level in different inter-tropical systems was collected in the Laboratory of Reproductive Biology at Universidad de Caldas (Colombia) during seven years. The database included the seminal variables: concentration, morphology (head, midpiece and tail), membrane structural integrity (MSI), acrosome integrity (ACRI), and membrane functional integrity (MFI) proximal citoplasmic droplets (PCD), distal citoplasmic droplets (DCD), and total citoplasmic droplets (TCD). Additionally, the racial group of the male (grac): maternal cross (cm), paternal cross (cp), paternal pure (pp), maternal pure (pm) and age group (agegr): young (up to 18 months), mature (19 to 36 months) and old (over 37 months) were registered. Data was explored by estimating descriptive statistics; it was analyzed using a log-linear model consisting of a Poisson regression with a log link function using the PROC GENMOD of SAS (SAS Inst. Cary, NC). The effect of age group and racial group on semen quality variables using a mixed model for repeated measurements in the same boar over time were evaluated. The Spearman correlation coefficients between variables were calculated. The variables: macro, micro and pyriform head; double, eccentric, and thickened midpiece; tail whip; ladder bow; ball and distal reflex showed less than 1% prevalence. The highest values of FMI were found in cm and pp. Males cp, cm and pm do not differ from each other in MFI, but all had higher values than pp. The ACRI in cm was higher than cp, and these higher than pm. Presentation of TCD increased with age, with a higher percentage of PCD in older animals than in younger animals; mature animals showed intermediate values between them. The TCD exceeded 15% of presentation, being always higher for PCD than for GCP. The TCD is negatively correlated with MSI and ARCI, but positively correlated with MFI. The same trend is observed in the correlations with distal cytoplasmic droplets. However, the correlation of proximal droplets with the structural integrity loses significance, though they are negatively correlated with acrosome integrity. Values for MSI, MFI and ACRI, do not limit the quality of the samples in this population.
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References
ALTHOUSE, G.C. & LU, K.G., 2005.- Bacteriospermia in extended porcine semen. Theriogenology, 63 (2): 573-584. doi: 10.1016/j.theriogenology.2004.09.031
AMANN, R.P., 1989.- Can the Fertility Potential of a Seminal Sample Be Predicted Accurately? Journal of andrology, 10 (2): 89-98.
ARDÓN, F., EVERT, M., BEYERBACH, M., WEITZE, K.F. & WABERSKI, D., 2005.- Accesory Sperm: A Biomonitor of Boar Sperm Fertilization Capacity. Theriogenology, 63: 1891-1901.
BRIZ, M., BONET, S., PINART, B., EGOZCUE, F. & CAMPS, R., 1995.- Comparative Study of Boar Sperm Coming From the Caput, Corpus, and Cauda Regions of the Epididymis. Journal of andrology, 16 (2): 175-188.
DÍAZ, F.O., MESA, H., VALENCIA MEJÍA, J.G., GÓMEZ LONDOÑO, G. & HENAO URIBE, F.J., 2009.- Evaluación de la integridad acrosomal y la funcionalidad bioquímica de la membrana espermática en cerdos reproductores con gotas citoplásmicas persistentes. Revista Cientifica, Facultad de Ciencias Veterinarias, Universidad del Zulia, 19 (5): 500-505.
DRUART, X., GATTI, J.-L., HUET, S., DACHEUX, J.-L. & HUMBLOT, P., 2009.- Hypotonic resistance of boar spermatozoa: sperm subpopulations and relationship with epididymal maturation and fertility. Reproduction, 137: 205-213.
FISCHER, K.A., VAN LEYEN, K., LOVERCAMP, K.W., MANANDHAR, G., SUTOVSKY, M., FENG, D., .SUTOVSKY, P., 2005.- 15-Lipoxygenase is a component of the mammalian sperm cytoplasmic droplet. Reproduction, 130 (2): 213-222. doi: 10.1530/rep.1.00646
FLOWERS, W., 2002.- Increasing Fertilization Rate of Boars: Influence of number and Quality of Spermatozoa Inseminated. Animal Science, 80 (Suppl.1): E47-E53.
FRESHMAN, J.L., 2002.- Semen collection and evaluation. Clin Tech Small Anim Pract, 17 (3): 104-107. doi: 10.1053/svms.2002.34326
GADEA, J., SELLES, E. & MARCO, M.A., 2004.- The predictive value of porcine seminal parameters on fertility outcome under commercial conditions. Reproduction in domestic animals = Zuchthygiene , 39 (5): 303-308. doi: 10.1111/j.1439-0531.2004.00513.x
HENAO, F.J., VALENCIA, J., DÍAZ, O. & RANGEL, M., 2010.- Efecto de la adición de plasma seminal sobre la eliminación de gotas citoplásmicas en semen de Sus scrofa (Linnaeus, 1758). Boletin Científico Museos Historia Natural, 15 (2): 94-104.
JOHNSON, L., WEITZE, K.F., FISER, P. & MAXWELL, W.M., 2000.- Storage of boar semen. Animal reproduction science, 62: 143-172.
KOKETSU, Y. & SASAKI, Y., 2008.- Boar culling and mortality in commercial swine breeding herds. Theriogenology, 71: 1186-1191.
KONDRACKI, S., BANASZEWSKA, D. & MIELNICKA, C., 2005.- The effect of age on the morphometric sperm traits of domestic pigs (Sus scrofa domestica). Cellular and molecular biology letters, 10: 3-13.
KUSTER, C.E., HESS, R.A. & ALTHOUSE, G.C., 2004.- Immunofluorescence reveals ubiquitination of retained distal cytoplasmic droplets on ejaculated porcine spermatozoa. Journal of andrology, 25 (3): 340-347.
LÓPEZ-RODRÍGUEZ, A., 2012.- Fresh boar semen: quality control and production. (PhD), Ghent University, Belgium.
LOVERCAMP, K.W., SAFRANSKI, T.J., FISCHER, K.A., MANANDHAR, G., SUTOVSKY, M., HERRING, W. & SUTOVSKY, P., 2007.- High resolution light microscopic evaluation of boar semen quality sperm cytoplasmic droplet retention in relationship with boar fertility parameters. Archives of Andrology, 53 (4): 219-233.
MASENYA, M.B., MPHAPHATHI, M.L., MAPEKA, M.H., MUNYAI, P.H., MAKHAFOLA, M.B., RAMUKHITHI, F.V., NEDAMBALE, T.L., 2011.- Comparative study on semen characteristics of Kolbroek and Large White boars following computer aided sperm analysis® (CASA). African Journal of Biotechnology, 10 (64): 14223-14229. doi: 10.5897/AJB11.2010
OSORIO-SERNA, R.E., GIRALDO, J.F., MESA, H., GÓMEZ, G. & HENAO, F.J., 2007.- Evaluación de la integridad acrosómica en semen de verraco. Vet. Zootec., 1 (1): 41-47.
PÉREZ-LLANO, B., LORENZO, J.L., YENES, P., TREJO, A. & GARCÍA-CASADO, P., 2001.- A short hypoosmotic swelling test for the prediction of boar sperm fertility. Theriogenology, 56 (3): 387-398.
PIZZI, F., GLIOZZI, T.M., CEROLINI, S., MALDJIAN, A., ZANIBONI, L., PARODI, L. & GANDINI, G., 2005.- Semen quality of Italian local pig breed. Ital. J. Anim. Sci., 4 (2): 482-484.
POPWELL, J.M. & FLOWERS, W.L., 2004.- Variability in relationships between semen quality and estimates of in vivo and in vitro fertility in boars. Animal Reproduction Science, 81: 97-113.
PURSEL, V.G., JOHNSON, L.A. & RAMPACECK, G.B., 1972.- Acrosome morphology of boar spermatozoa incubated before cold shock. J. Anim. Sci., 34: 278-283.
ROBINSON, J.A.B. & BUHR, M.M., 2005.- Impact of genetic selection on management of boar replacement. Theriogenology, 63: 668-678.
RUTTEN, S., MORRISON, R. & REICKS, D., 2000.- Boar stud producion analysis. Swine Health and Production, 8 (1): 11-14.
SAFRANSKI, T.J., 2008.- Genetic selection of boars. Theriogenology, 70: 1310-1316.
SMITAL, J., 2010.- Comparison of Environmental Variations in Boar Semen Characteristics of Six Breeds And Their Crossbreds Over an Eight-Year Period. Research In Pig Breeding, 4 (1): 26-32.
SUTKEVICIENE, N. & ZILINSKAS, H., 2004.- Sperm morphology and fertility in artificial insemination boars. Veterinarija ir Zootechnika, 26 (48): 11-13.
TURBA, M.E., FANTINATI, P., BERNARDINI, C., GENTILINI, F., BACCI, M.L. & FORNI, M., 2007.- Relationships between innovative and traditional parameters to investigate semen quality in pigs. Animal reproduction science, 99: 72-81.
WYSOKINSKA, A. & KONDRACKI, S., 2013.- Assessment of the effect of heterosis on semen parameters of two-breed crosses of Duroc, Hampshire and Pietrain boars. Archiv Tierzucht, 56(7): 1-10. doi: 10.7482/0003-9438-56-007
