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Fecha Publicación: 2011-07-01
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Henao Uribe, F. J., Valencia Giraldo, J. A., Díaz Franco, O., & Rangel Sierra, M. Y. (2011). Efecto de la adición de plasma seminal sobre la eliminación de gotas citoplásmicas en semen de Sus Scrofa Linaeus, 1758. Boletín Científico Centro De Museos Museo De Historia Natural, 15(2), 94–104. Recuperado a partir de https://revistasojs.ucaldas.edu.co/index.php/boletincientifico/article/view/4611
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Las gotas citoplásmicas (GCs) son remanentes del citoplasma que quedan adheridos al espermatozoide después de la espermatogénesis, constituyen la anormalidad espermática más frecuente en porcinos, y se relacionan claramente con baja fertilidad. Hay serios indicios de que la fructosa y el AMPc del plasma seminal intervienen en la maduración espermática, en el desprendimiento de las GCs, y en la reacción acrosómica. El objetivo del presente estudio fue evaluar el efecto de la adición de plasma seminal, en el desprendimiento de las GCs en machos con diagnóstico de persistencia de las mismas. En el estudio se emplearon tres verracos (dos con persistencia de GCs y uno normal) de tres a cinco años de edad, alojados en la granja Montelindo de la Universidad de Caldas; a los cuales se les realizaron análisis seminales semanales, completos, durante cuatro meses. Se llevó a cabo un arreglo factorial 3x3x2 (adición a la FR de los machos con persistencia de GCs de 0%, 20% de PSMS y 20% de PSMGCs; 0, 60 y 120 minutos de incubación, y 16 y 37°C de temperaturas de incubación) en un diseño de bloques completos al azar, analizado mediante análisis de varianza y prueba de Tukey. La incubación del semen de machos con persistencia de GCs con PSMGCs redujo más del 4% las GCs respecto a la incubación sin PS y con PSMS; igualmente se registró reducción de aproximadamente el 5% en las GCs, al aumentar el tiempo de incubación de 0 a 120 minutos, y alrededor de 2% al llevar la temperatura de incubación de 16 a 37°C.
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ADACHI, J., TATE, S. & MIYAKE, M., 2008. Effects of protein phosphatase inhibitor calyculin a on the postacrosomal protein serine/threonine phosphorylation state and acrosome reaction in boar spermatozoa incubated with a cAMP analog. J Reprod Dev., 54 (3): 171-6.
BERGER, T. & CLEGG, E.D., 1985. Effect of male accessory gland secretions on sensitivity of porcine sperm acrosomes to cold shock, initiation of motility and loss of cytoplasmic droplets. J Anim Sci., 60: 1295-1302.
BONET, S., BRIZ, M. & FRADERA, A., 1993. Ultrastructural abnormalities of boar spermatozoa. Theriogenology, 40: 383-396.
CABALLERO, I., 2007. Estudio del plasma seminal y la espermadhesina PSP-I/PSP-II sobre la funcionalidad de los espermatozoides de verraco: Tesis, Universidad de Murcia, Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, España.
CABALLERO, I., VÁZQUEZ, J.M., GIL. M.A., CALVETE, J.J., ROCA, J., SANZ, L., PARRILLA, I., GARCÍA, E.M., RODRÍGUEZ-MARTÍNEZ, H. & MARTÍNEZ, E.A., 2004. Does seminal plasma psp-i/psp-ii spermadhes in modulate the ability of boar spermatozoa to penetrate homologous oocytes in vitro? J Androl., 25: 1004-1012.
CALVETE, J.J., DOSTÁLOVÁ, Z., SANZ, L., ADERMANN, K., THOLE, H.H., TÖPFER-PETERSEN, E., 1996. Mapping the heparin-binding domain of boar spermadhesins. FEBS Letters, 379: 207-211.
CALVETE, J.J., ENSSLIN, M., MBURU, J., IBORRA, A., MARTÍNEZ, A., ADERMANN, K., WABERSKI, D., SANZ, L., TÖPFER-PETERSEN, E., WEITZE, K.F., EINARSSON, S. & RODRÍGUEZ-MARTÍNEZ, E., 1997. Monoclonal antibodies against boar sperm zonapellucida-binding protein awn-1. Characterization of a continuous antigenic determinant and immunolocalization of awn epitopes in inseminated sows. Biol Reprod., 57: 735-742.
CENTURIÓN, F., VÁZQUEZ, J.M., CALVETE, J.J., ROCA, J., SANZ, L., PARRILLA, I., GARCÍA, E.M. & MARTÍNEZ, E.A., 2003. Influence of porcine spermadhesins on the susceptibility of boar spermatozoa to high dilution. Biol Reprod., 69: 640-646.
EKHLASI-HUNDRIESER, M., SINOWATZ, F., GREISER DE WILKE, I., WABERSKI D. & TÖPFER-PETERSEN, E., 2002. Expression of spermadhesin genes in porcine male and female reproductive tracts. Mol Reprod Dev., 61 (1): 32-41.
FISCHER, K.A., LEYEN, K.V., LOVERCAMP, K.W., MANANDHAR, G., SUTOVSKY, M., FENG, D., SAFRANSKI, T. & SUTOVSKY, P., 2005. 15-lipoxygenase is a component of the mammalian sperm cytoplasmic droplet. Reproduction, 130: 213-222.
FOLEY, C.W., HEIDENREICH, C.J., HARRINGTON, R.B., JONES, H.W. & ERB, R.E., 1964. Changes in fructose, lacticacid, ph, and motility of boar semen during incubation at 37°C. J Anim Sci., 23: 558-561.
GARCÍA, E.M., 2007. Análisis, función y aplicaciones biotecnológicas de las proteínas del plasma seminal de porcino PSP I y PSP II: Tesis, Universidad de Murcia, Facultad de Veterinaria, Departamento de Medicina y Cirugía Animal, España.
GARCÍA, J.C., DOMÍNGUEZ, J.C., PEÑA, F.J., ALEGRE, B., GONZÁLEZ, R., CASTRO, M.J., HABING, G.G. & KIRKWOOD, R.N., 2010. Thawing boar semen in the presence of seminal plasma: Effects on sperm quality and fertility. Anim Reprod Sci., 119: 160-165.
GARNER, D.L., THOMAS, C.A., GRAVANCE, C.G., MARSHALL C.E., DEJARNETTE, J.M. & ALLEN, C.H., 2001. Seminal plasma addition attenuates the diltion effect in bovine sperm. Theriogenology, 56: 31-40.
GÓMEZ, G., 2010. Dinámica de la calidad seminal de verracos del centro-occidente colombiano: Tesis, Universidad de Caldas, Maestría en Sistemas de Producción Agropecuaria, Manizales, Colombia.
HARAYAMA, H., SHIBUKAWA, T., MIYAKE, M., KANNAN, Y. & KATO, S., 1996. Fructose stimulates shedding of cytoplasmic droplets from epididymal boar spermatozoa. Reprod Fertil Dev., 8: 1039-1043.
JONÁKOVÁ, V. & TICHÁ, M., 2004. Boar seminal plasma proteins and their binding properties. A review Collection of Czechoslovak Chemical Communications, 69: 461-475.
KAPLAN, M., RUSSELL, L.D., PETERSON, R.N. & MARTAN, J., 1984. Boar sperm cytoplasmic droplets: Their ultrastructure, their numbers in the epididymis and at ejaculation and their removal during isolation of sperm plasma membranes. Tissue and Cell, 16: 455-468.
KATO, S., SHIBUKA, W.A.T., HARAYAMA, H., et al., 1996. Timing of shedding and dissentegration of cytoplasmic droplet from boar and goat spermatozoa. J. Reprod. Dev., 42: 237-241.
KAWANO, N., SHIMADA, M. & TERADA, T., 2004. Motility and penetration competence of frozen-thawed miniature pig spermatozoa are substantially altered by exposure to seminal plasma before freezing. Theriogenology, 61: 351-364.
LOVERCAMP, K.W., SAFRANSKY, T.J. & FISCHER, K.A., 2007. High resolution light microscopic evaluation of boar semen quality sperm cytoplasmic droplet retention in relationship with boar fertility parameters. Arch Androl, 53: 219-228.
OKAMURA, N., DACHEUX, F., VENIEN, A., ONOE, S., HUET, J.C. & DACHEUX, J.L., 1992. Localization of a Maturation-Dependent Epididymal Sperm Surface Antigen Recognized by a Monoclonal Antibody Raised against a 135-Kilodalton Protein in Porcine Epididymal Fluid. Biol Reprod., 47: 1040-1052.
PÉREZ-LLANO, B., LORENZO, J.L., YENES, P., TREJO, A. & GARCÍA-CASADO, P. 2001. Short hypoosmotic swelling test for the prediction of boar sperm fertility. Theriogenology, 56: 387-398.
PURSEL, V.G. & JOHNSON, L.A., 1974. Glutaraldehyde fixation of boar spermatozoa for acrosome evaluation. Theriogenology, 1: 63-68.
RODRÍGUEZ-MARTÍNEZ, H., SARAVIA, F., WALLGREN, M., TIENTHAI, P., JOHANNISSON, A., VÁZQUEZ, J.M., MARTÍNEZ, E., ROCA, J., SANZ, L. & CALVETE, J.J., 2005. Boar spermatozoa in the oviduct. Theriogenology, 63:514-535.
ROZEBOOM, K.J., 2000. Evaluating of boar semen quality (en) N. C. S. UNIVERSITY (ed.) Animal Science Facts. Extension Swine Husbandry, North Carolina State, 1-8.
SALA-ECHAVE, R., REGUERA, G., GARCÍA-CASADO, P. & PÉREZ-LLANO, B., 2008. Influencia del calentamiento de las dosis seminales porcinas sobre la calidad espermática durante la I.A. Av. Tecnol. porc., 5 (3): 34-41.
SARAVIA, F., WALLGREN, M., JOHANNISSON, A., CALVETE, J.J., SANZ, L., PEÑA, F.J., ROCA, J. & RODRÍGUEZ-MARTÍNEZ, H., 2009. Exposure to the seminal plasma of different portions of the boar ejaculate modulates the survival of spermatozoa cryopreserved in MiniFlatPacks. Theriogenology, 71: 662-675.
WABERSKI, D., SÜDHOFF, H., HAHN, T., JUNGBLUT, P.W., KALLWEIT, E., CALVETE, J.J., ENSSLIN, M., HOPPEN, H.O., WINTERGALEN, N. & WEITZE, K.F., 1995. Advanced ovulation in gilts by the intrauterine application of a low molecular mass pronase-sensitive fraction of boar seminal plasma. J Reprod Fertil., 105: 247-252.
ZOU, C.X. & YANG, Z.M., 2000. Evaluation on sperm quality of freshly ejaculated boar semen during in vitro storage under different temperatures. Theriogenology, 53: 1477-1488.
BERGER, T. & CLEGG, E.D., 1985. Effect of male accessory gland secretions on sensitivity of porcine sperm acrosomes to cold shock, initiation of motility and loss of cytoplasmic droplets. J Anim Sci., 60: 1295-1302.
BONET, S., BRIZ, M. & FRADERA, A., 1993. Ultrastructural abnormalities of boar spermatozoa. Theriogenology, 40: 383-396.
CABALLERO, I., 2007. Estudio del plasma seminal y la espermadhesina PSP-I/PSP-II sobre la funcionalidad de los espermatozoides de verraco: Tesis, Universidad de Murcia, Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, España.
CABALLERO, I., VÁZQUEZ, J.M., GIL. M.A., CALVETE, J.J., ROCA, J., SANZ, L., PARRILLA, I., GARCÍA, E.M., RODRÍGUEZ-MARTÍNEZ, H. & MARTÍNEZ, E.A., 2004. Does seminal plasma psp-i/psp-ii spermadhes in modulate the ability of boar spermatozoa to penetrate homologous oocytes in vitro? J Androl., 25: 1004-1012.
CALVETE, J.J., DOSTÁLOVÁ, Z., SANZ, L., ADERMANN, K., THOLE, H.H., TÖPFER-PETERSEN, E., 1996. Mapping the heparin-binding domain of boar spermadhesins. FEBS Letters, 379: 207-211.
CALVETE, J.J., ENSSLIN, M., MBURU, J., IBORRA, A., MARTÍNEZ, A., ADERMANN, K., WABERSKI, D., SANZ, L., TÖPFER-PETERSEN, E., WEITZE, K.F., EINARSSON, S. & RODRÍGUEZ-MARTÍNEZ, E., 1997. Monoclonal antibodies against boar sperm zonapellucida-binding protein awn-1. Characterization of a continuous antigenic determinant and immunolocalization of awn epitopes in inseminated sows. Biol Reprod., 57: 735-742.
CENTURIÓN, F., VÁZQUEZ, J.M., CALVETE, J.J., ROCA, J., SANZ, L., PARRILLA, I., GARCÍA, E.M. & MARTÍNEZ, E.A., 2003. Influence of porcine spermadhesins on the susceptibility of boar spermatozoa to high dilution. Biol Reprod., 69: 640-646.
EKHLASI-HUNDRIESER, M., SINOWATZ, F., GREISER DE WILKE, I., WABERSKI D. & TÖPFER-PETERSEN, E., 2002. Expression of spermadhesin genes in porcine male and female reproductive tracts. Mol Reprod Dev., 61 (1): 32-41.
FISCHER, K.A., LEYEN, K.V., LOVERCAMP, K.W., MANANDHAR, G., SUTOVSKY, M., FENG, D., SAFRANSKI, T. & SUTOVSKY, P., 2005. 15-lipoxygenase is a component of the mammalian sperm cytoplasmic droplet. Reproduction, 130: 213-222.
FOLEY, C.W., HEIDENREICH, C.J., HARRINGTON, R.B., JONES, H.W. & ERB, R.E., 1964. Changes in fructose, lacticacid, ph, and motility of boar semen during incubation at 37°C. J Anim Sci., 23: 558-561.
GARCÍA, E.M., 2007. Análisis, función y aplicaciones biotecnológicas de las proteínas del plasma seminal de porcino PSP I y PSP II: Tesis, Universidad de Murcia, Facultad de Veterinaria, Departamento de Medicina y Cirugía Animal, España.
GARCÍA, J.C., DOMÍNGUEZ, J.C., PEÑA, F.J., ALEGRE, B., GONZÁLEZ, R., CASTRO, M.J., HABING, G.G. & KIRKWOOD, R.N., 2010. Thawing boar semen in the presence of seminal plasma: Effects on sperm quality and fertility. Anim Reprod Sci., 119: 160-165.
GARNER, D.L., THOMAS, C.A., GRAVANCE, C.G., MARSHALL C.E., DEJARNETTE, J.M. & ALLEN, C.H., 2001. Seminal plasma addition attenuates the diltion effect in bovine sperm. Theriogenology, 56: 31-40.
GÓMEZ, G., 2010. Dinámica de la calidad seminal de verracos del centro-occidente colombiano: Tesis, Universidad de Caldas, Maestría en Sistemas de Producción Agropecuaria, Manizales, Colombia.
HARAYAMA, H., SHIBUKAWA, T., MIYAKE, M., KANNAN, Y. & KATO, S., 1996. Fructose stimulates shedding of cytoplasmic droplets from epididymal boar spermatozoa. Reprod Fertil Dev., 8: 1039-1043.
JONÁKOVÁ, V. & TICHÁ, M., 2004. Boar seminal plasma proteins and their binding properties. A review Collection of Czechoslovak Chemical Communications, 69: 461-475.
KAPLAN, M., RUSSELL, L.D., PETERSON, R.N. & MARTAN, J., 1984. Boar sperm cytoplasmic droplets: Their ultrastructure, their numbers in the epididymis and at ejaculation and their removal during isolation of sperm plasma membranes. Tissue and Cell, 16: 455-468.
KATO, S., SHIBUKA, W.A.T., HARAYAMA, H., et al., 1996. Timing of shedding and dissentegration of cytoplasmic droplet from boar and goat spermatozoa. J. Reprod. Dev., 42: 237-241.
KAWANO, N., SHIMADA, M. & TERADA, T., 2004. Motility and penetration competence of frozen-thawed miniature pig spermatozoa are substantially altered by exposure to seminal plasma before freezing. Theriogenology, 61: 351-364.
LOVERCAMP, K.W., SAFRANSKY, T.J. & FISCHER, K.A., 2007. High resolution light microscopic evaluation of boar semen quality sperm cytoplasmic droplet retention in relationship with boar fertility parameters. Arch Androl, 53: 219-228.
OKAMURA, N., DACHEUX, F., VENIEN, A., ONOE, S., HUET, J.C. & DACHEUX, J.L., 1992. Localization of a Maturation-Dependent Epididymal Sperm Surface Antigen Recognized by a Monoclonal Antibody Raised against a 135-Kilodalton Protein in Porcine Epididymal Fluid. Biol Reprod., 47: 1040-1052.
PÉREZ-LLANO, B., LORENZO, J.L., YENES, P., TREJO, A. & GARCÍA-CASADO, P. 2001. Short hypoosmotic swelling test for the prediction of boar sperm fertility. Theriogenology, 56: 387-398.
PURSEL, V.G. & JOHNSON, L.A., 1974. Glutaraldehyde fixation of boar spermatozoa for acrosome evaluation. Theriogenology, 1: 63-68.
RODRÍGUEZ-MARTÍNEZ, H., SARAVIA, F., WALLGREN, M., TIENTHAI, P., JOHANNISSON, A., VÁZQUEZ, J.M., MARTÍNEZ, E., ROCA, J., SANZ, L. & CALVETE, J.J., 2005. Boar spermatozoa in the oviduct. Theriogenology, 63:514-535.
ROZEBOOM, K.J., 2000. Evaluating of boar semen quality (en) N. C. S. UNIVERSITY (ed.) Animal Science Facts. Extension Swine Husbandry, North Carolina State, 1-8.
SALA-ECHAVE, R., REGUERA, G., GARCÍA-CASADO, P. & PÉREZ-LLANO, B., 2008. Influencia del calentamiento de las dosis seminales porcinas sobre la calidad espermática durante la I.A. Av. Tecnol. porc., 5 (3): 34-41.
SARAVIA, F., WALLGREN, M., JOHANNISSON, A., CALVETE, J.J., SANZ, L., PEÑA, F.J., ROCA, J. & RODRÍGUEZ-MARTÍNEZ, H., 2009. Exposure to the seminal plasma of different portions of the boar ejaculate modulates the survival of spermatozoa cryopreserved in MiniFlatPacks. Theriogenology, 71: 662-675.
WABERSKI, D., SÜDHOFF, H., HAHN, T., JUNGBLUT, P.W., KALLWEIT, E., CALVETE, J.J., ENSSLIN, M., HOPPEN, H.O., WINTERGALEN, N. & WEITZE, K.F., 1995. Advanced ovulation in gilts by the intrauterine application of a low molecular mass pronase-sensitive fraction of boar seminal plasma. J Reprod Fertil., 105: 247-252.
ZOU, C.X. & YANG, Z.M., 2000. Evaluation on sperm quality of freshly ejaculated boar semen during in vitro storage under different temperatures. Theriogenology, 53: 1477-1488.
