Evaluación in vitro de la viabilidad del semen porcino hasta 120 horas de almacenamiento en refrigeración

Abstract

ABSTRACT: The aim of the present study was to evaluate the changes in pH, morphology, acrosomal integrity and sperm survival, of BTS diluted and refrigerated semen of 11 boars (seven paternal and four maternal line), every 24 hours, from 0 h (reception time) until 120 h, three times at monthly intervals. Statistical analysis were carried out with PROC GLM, MIXED and GENMOD of SAS using a mixed model to evaluate the effects of storage time (TA) and genetic line, on the evaluated variables. A z2 test was used to evaluate TA effect on detritus and agglutination cells. At 120 h, the sperm cells with normal acrosome diminished; the maternal line has 24.3% less probabilities of showing sperm agglutination. No significant effect of line and TA was identified on morphology, pH, sperm survival and detritus cell presence. It was concluded that boar semen diluted in BTS, with 3x109 spermatozoa and stored at 17ºC, maintains indicative viability characteristics up  to 120 h of storage. In vitro semen evaluation is a tool that offers the chance of using seminal doses otherwise wasted.

Keywords:

calidad espermática

almacenamiento de semen

porcinos

acrosomal

spermatic quality

boar

swine

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