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Publication Date: 2010-12-17
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Villa Arcila, N. A. ., Ceballos Márquez, A. ., Duque Madrid, P. C. ., & Restrepo Restrepo, L. G. . (2010). Determination of antioxidant status in blood serum and seminal plasma in dogs. Revista Veterinaria Y Zootecnia (On Line), 4(2), 30–36. Retrieved from https://revistasojs.ucaldas.edu.co/index.php/vetzootec/article/view/5595
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Abstract
ABSTRACT: The study was conducted with twenty adult clinically healthy dogs from Manizales, fed with balanced commercial dog food, to know the activity of antioxidant enzymes: glutathione peroxidase (GSH-Px, EC 1.11.1.9) and superoxide dismutase (SOD, EC 1.15.1.1) in blood, serum, and seminal plasma. In addition the relationship of these enzymes with semen characteristics (volume, motility, sperm concentration) and hyposmotic test (PH) were studied. The GSH-Px showed 173.0±74.4 Ug / Hb activity in blood, 1.3±0.9 U/L in serum of and 1.0±0.5 U/L in seminal plasma. The GSH-Px activity in seminal plasma was negatively correlated with sperm volume (r = -0.45, P=0.04); no correlations were obtained with the other variables tested (P>0.05). With regards to SOD, 573.4±171.1 Ug/Hb activity was found in blood, 85.2±28.6 was found in serum and 3.2±1.9 U/mL was found in seminal plasma. A positive correlation of this enzyme in seminal plasma with sperm concentration (r = 0.47, P=0.03) was also found. There were no significant correlations (P>0.05) in the other tested variables. The activity of the GSH-Px and SOD enzymes in blood, serum and seminal plasma
were not related to sperm quality, except in SOD activity in seminal plasma with sperm concentration; besides, the activity of enzymes in serum, blood and seminal plasma are not correlated suggesting that they act as independent systems.
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References
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Armstrong, J.S.; Rajasekaran, M.; Chamulitrat, W.; Gatti, P.; Hellstrom, W.J.; Sikka, S.C. Characterization of reactive oxygen species induced effects on human spermatozoa
movement and energy metabolism. Free Radical Biology and Medicine, 26(7-8), p.869- 880, 1999.
Cassani. P; Beconi, M.T; Flaherty, C. relationship between total superoxide disminutase activity with lipid peroxidation, diamics and morphological parameters in canine semen. Animal Reproduction Science, 86, p.163- 173, 2005.
Ceballos, A. Actividad sanguínea de GSH-PX como indicador de Estrés Metabólico Nutricional de SE en rebaños lecheros. Valdivia, Chile: Universidad Austral, Facultad de Ciencias Veterinarias, Instituto de Ciencias Veterinarias, 1996.
Gavella, M.; Lipovac, V.; Vucic, M.; Rocic, B. Superoxide anion scavenging capacity of human seminal plasma. International Journal of Andrology, 19(2), p.82-90, 1996.
Gavella, M.; Lipovac, V.; Vucic, M.; Sverko, V. In vitro inhibition of superoxide anion production and superoxide dismutase activity by zinc in human spermatozoa. International Journal of Andrology, 22(4), p.266-274, 1999.
Griveau, J.F.; Lannou, D. Reactive oxygen species and human spermatozoa: physiology and pathology. International Journal of Andrology, 20(2), p.61-69, 1997.
Hatamoto, L.K; Baptista, C.A; Nichi, M; Barnabe, V.H; Barnabe, R.C; Cortada, C.N. Effects of dexamethasone treatment (to mimic stress) and Vitamin E oral supplementation
on the spermiogram and on seminal plasma spontaneous lipid peroxidation and antioxidant enzyme activities in dogs. Theriogenology, 66(6-7), p.1610-1614, 2006.
Hernández, S.R.; Guzmán, A.M.; Hicks, J.J. Oxygen reactive species in spermatozoa. Ginecol. Obstet. Mex., 63, p.50-54, 1995.
Iwanier, K.; Zachara, B.A. Selenium supplementation enhances the element concentration in blood and seminal fluid but does not change the spermatozoal quality characteristics in subfertile men. International Journal of Andrology, 16(5), p.441-447, 1995.
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Kelso, K.A.; Redpath A.; Noble, R.C.; Speake, B.K. Lipid and antioxidant changes in spermatozoa and seminal plasma throughout the reproductive period of bulls. Journal of Reproduction and Fertility, 109(1), p.1-6, 1997.
Kim, J.G.; Parthasarathy, S. Oxidation and the spermatozoa. Semin. Reprod. Endocrinol., 16(4), p.235-239, 1998.
Lamirande, E.; Gagnon, C. Capacitation-associated production of superoxide anion by human spermatozoa. Free Radical Biology and Medicine, 18(3), p.487-495, 1995.
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Santanam, N.; Ramachandran, S.; Parthasarathy, S. Oxygen radicals, antioxidants, and lipid peroxidation. Semin. Reprod. Endocrinol., 16(4), p.275-280, 1998.
Scott, R.; Macpherson, A.; Yates, R.W.; Hussain, B.; Dixon, J. The effect of oral selenium supplementation on human sperm motility. Br. Journal of Urology, 82(1), p.76-80, 1998.
Sikka, S.C. Oxidative stress and role of antioxidants in normal and abnormal spermfunction. Front. Biosci., 1, 78-86, 1996.
Sikka, S.C.; Rajasekaran, M.; Hellstrom, W.J. Role of oxidative stress and antioxidants in male infertility. Journal of Andrology, 16(6), p.464-481,1995.
Slaweta, R.; Laskowska, T.; Szymanska, E. Lipid peroxides, spermatozoa quality and activity of glutatione peroxidase in bull semen. Acta Physiol. Pol., 39(3), p.207-214, 1998.
Surai, P.F.; Blesbois, E.; Grasseau, I; Chalah, T; Brillard, J.P.; Wishart, G.J.; Cerolini, S.; Sparks, N.H. Fatty acid composition, glutathione peroxidase and superoxide dismutase activity and total antioxidant activity of avian semen. Comp. Biochem Physiol. B. Biochem. Mol. Biol., 120(3), p.527-533, 1998.
Twigg, J.; Fulton, N.; Gomez, E.; Irvine, D.S.; Aitken, R.J. Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: lipid peroxidation, DNA fragmentation and effectiveness of antioxidants. Human Reproduction, 13(6), p.1429-1436, 1998.
Villa, N.A.; Ceballos, A.; Correa, S.; López, E.; Salazar, J. Evaluación del estado antioxidante en plasma seminal en toros Brahman (Bos indicus) en pastoreo y suplementados. Revista Colombiana de Ciencias Pecuarias, 12, 187, 1999a (suplemento).
Villa, N.A.; Ceballos, A.; Sánchez, L.; Salazar, J.I.; Sánchez, L.E.; Sánchez, R.; Gómez, A.M. Determinación del estado antioxidante en sangre y plasma seminal en porcinos.
Manizales, Colombia: Universidad de Caldas, 1999b. Tesis (Médico Veterinario Zootecnista).
Yeung, C.H.; Cooper, T.G.; De Geyter, M.; De Geyter, C.; Rolf, C.; Kamischke, A.; Nieschlag, E. Studies on the origin of redox enzymes in seminal plasma and their relationship with results of in-vitro fertilization. Mol. Human Reproduction, 4(9), p.835-839, 1998.
function. Free Radical Biology and Medicine, 21(4), p.495-504, 1996.
Aitken, R.J.; Fisher, H. Reactive oxygen species generation and human spermatozoa: The balance of benefit and risk. BioEssays, 16, p.259-267, 1994.
Armstrong, J.S.; Rajasekaran, M.; Chamulitrat, W.; Gatti, P.; Hellstrom, W.J.; Sikka, S.C. Characterization of reactive oxygen species induced effects on human spermatozoa
movement and energy metabolism. Free Radical Biology and Medicine, 26(7-8), p.869- 880, 1999.
Cassani. P; Beconi, M.T; Flaherty, C. relationship between total superoxide disminutase activity with lipid peroxidation, diamics and morphological parameters in canine semen. Animal Reproduction Science, 86, p.163- 173, 2005.
Ceballos, A. Actividad sanguínea de GSH-PX como indicador de Estrés Metabólico Nutricional de SE en rebaños lecheros. Valdivia, Chile: Universidad Austral, Facultad de Ciencias Veterinarias, Instituto de Ciencias Veterinarias, 1996.
Gavella, M.; Lipovac, V.; Vucic, M.; Rocic, B. Superoxide anion scavenging capacity of human seminal plasma. International Journal of Andrology, 19(2), p.82-90, 1996.
Gavella, M.; Lipovac, V.; Vucic, M.; Sverko, V. In vitro inhibition of superoxide anion production and superoxide dismutase activity by zinc in human spermatozoa. International Journal of Andrology, 22(4), p.266-274, 1999.
Griveau, J.F.; Lannou, D. Reactive oxygen species and human spermatozoa: physiology and pathology. International Journal of Andrology, 20(2), p.61-69, 1997.
Hatamoto, L.K; Baptista, C.A; Nichi, M; Barnabe, V.H; Barnabe, R.C; Cortada, C.N. Effects of dexamethasone treatment (to mimic stress) and Vitamin E oral supplementation
on the spermiogram and on seminal plasma spontaneous lipid peroxidation and antioxidant enzyme activities in dogs. Theriogenology, 66(6-7), p.1610-1614, 2006.
Hernández, S.R.; Guzmán, A.M.; Hicks, J.J. Oxygen reactive species in spermatozoa. Ginecol. Obstet. Mex., 63, p.50-54, 1995.
Iwanier, K.; Zachara, B.A. Selenium supplementation enhances the element concentration in blood and seminal fluid but does not change the spermatozoal quality characteristics in subfertile men. International Journal of Andrology, 16(5), p.441-447, 1995.
Kay, V. .; Robertson, L. Hyperactivated motility of human spermatozoa: a rewiew of physilogical function and application in assisted reproduction. Human Reproduction, Update, 4(6), p.776-786, 1998.
Kelso, K.A.; Redpath A.; Noble, R.C.; Speake, B.K. Lipid and antioxidant changes in spermatozoa and seminal plasma throughout the reproductive period of bulls. Journal of Reproduction and Fertility, 109(1), p.1-6, 1997.
Kim, J.G.; Parthasarathy, S. Oxidation and the spermatozoa. Semin. Reprod. Endocrinol., 16(4), p.235-239, 1998.
Lamirande, E.; Gagnon, C. Capacitation-associated production of superoxide anion by human spermatozoa. Free Radical Biology and Medicine, 18(3), p.487-495, 1995.
Lipecka, K.; Grabowska, B.; Daniszewska, K. Enzymatic activity of superoxide dismutase in lymphocytes and erythropcytes in people of different ages. Med. Pr., 37(5), p.305-308,
1986.
Marin, J.; Mahan, D.C.; Chung, Y.K.; Pate, J.L.; Pope, W.F. Effects of dietary selenium and vitamin E on boar performance and tissues responses, semen quality, and subsequent fertilization rates in matures gilts. Journal of Animal Science, 75(11), p.2994-3003, 1997.
Olson, P.N. Recolección y evaluación del semen canino. Kirk Bonagura. Terapéutica Veterinaria de Pequeños Animales. Madrid: Interamericana - McGraw-Hill, 1994. p.1039-
1044.
Rodríguez, J.E.; Montserrat, A.; Rigau, T. Effects of hypoosmotic incubation on acrosome and tail structure on canine spermatozoa. Theriogenology, 42, p.815-829, 1993.
Sánchez, R.G. Estrés oxidativo y fertilidad. En: Estrés oxidativo y antioxidantes en la Salud y Nutrición Animal. Curso-seminario. Editores: Wittwer, F.M., Ceballos, A.M. Valdivia, 1997. p.4-7.
Santanam, N.; Ramachandran, S.; Parthasarathy, S. Oxygen radicals, antioxidants, and lipid peroxidation. Semin. Reprod. Endocrinol., 16(4), p.275-280, 1998.
Scott, R.; Macpherson, A.; Yates, R.W.; Hussain, B.; Dixon, J. The effect of oral selenium supplementation on human sperm motility. Br. Journal of Urology, 82(1), p.76-80, 1998.
Sikka, S.C. Oxidative stress and role of antioxidants in normal and abnormal spermfunction. Front. Biosci., 1, 78-86, 1996.
Sikka, S.C.; Rajasekaran, M.; Hellstrom, W.J. Role of oxidative stress and antioxidants in male infertility. Journal of Andrology, 16(6), p.464-481,1995.
Slaweta, R.; Laskowska, T.; Szymanska, E. Lipid peroxides, spermatozoa quality and activity of glutatione peroxidase in bull semen. Acta Physiol. Pol., 39(3), p.207-214, 1998.
Surai, P.F.; Blesbois, E.; Grasseau, I; Chalah, T; Brillard, J.P.; Wishart, G.J.; Cerolini, S.; Sparks, N.H. Fatty acid composition, glutathione peroxidase and superoxide dismutase activity and total antioxidant activity of avian semen. Comp. Biochem Physiol. B. Biochem. Mol. Biol., 120(3), p.527-533, 1998.
Twigg, J.; Fulton, N.; Gomez, E.; Irvine, D.S.; Aitken, R.J. Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: lipid peroxidation, DNA fragmentation and effectiveness of antioxidants. Human Reproduction, 13(6), p.1429-1436, 1998.
Villa, N.A.; Ceballos, A.; Correa, S.; López, E.; Salazar, J. Evaluación del estado antioxidante en plasma seminal en toros Brahman (Bos indicus) en pastoreo y suplementados. Revista Colombiana de Ciencias Pecuarias, 12, 187, 1999a (suplemento).
Villa, N.A.; Ceballos, A.; Sánchez, L.; Salazar, J.I.; Sánchez, L.E.; Sánchez, R.; Gómez, A.M. Determinación del estado antioxidante en sangre y plasma seminal en porcinos.
Manizales, Colombia: Universidad de Caldas, 1999b. Tesis (Médico Veterinario Zootecnista).
Yeung, C.H.; Cooper, T.G.; De Geyter, M.; De Geyter, C.; Rolf, C.; Kamischke, A.; Nieschlag, E. Studies on the origin of redox enzymes in seminal plasma and their relationship with results of in-vitro fertilization. Mol. Human Reproduction, 4(9), p.835-839, 1998.
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