Authors
Abstract
ABSTRACT: The study was conducted with twenty adult clinically healthy dogs from Manizales, fed with balanced commercial dog food, to know the activity of antioxidant enzymes: glutathione peroxidase (GSH-Px, EC 1.11.1.9) and superoxide dismutase (SOD, EC 1.15.1.1) in blood, serum, and seminal plasma. In addition the relationship of these enzymes with semen characteristics (volume, motility, sperm concentration) and hyposmotic test (PH) were studied. The GSH-Px showed 173.0±74.4 Ug / Hb activity in blood, 1.3±0.9 U/L in serum of and 1.0±0.5 U/L in seminal plasma. The GSH-Px activity in seminal plasma was negatively correlated with sperm volume (r = -0.45, P=0.04); no correlations were obtained with the other variables tested (P>0.05). With regards to SOD, 573.4±171.1 Ug/Hb activity was found in blood, 85.2±28.6 was found in serum and 3.2±1.9 U/mL was found in seminal plasma. A positive correlation of this enzyme in seminal plasma with sperm concentration (r = 0.47, P=0.03) was also found. There were no significant correlations (P>0.05) in the other tested variables. The activity of the GSH-Px and SOD enzymes in blood, serum and seminal plasma
were not related to sperm quality, except in SOD activity in seminal plasma with sperm concentration; besides, the activity of enzymes in serum, blood and seminal plasma are not correlated suggesting that they act as independent systems.
Keywords:
References
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