Abstract
The objective was to evaluate the effect of progesterone on pregnancy in fixed time artificial insemination (FTAI) synchronization protocols in buffaloes during favorable breeding season. 16 milking calved buffaloes were randomly assigned to one of the following protocols T1: synchronization using progesterone and; T2: conventional OvSynch protocol. Pregnancy was evaluated 28 days after of artificial insemination: ovarian response was determined by ultrasonography and progesterone and estradiol by enzyme linked immunosorbent assay (ELISA). Effect of progesterone on pregnancy rate was evaluated trough Chi square-test. T-student test was carried out to evaluate the effect of weight, milking days, progesterone and estradiol levels, on day of artificial insemination and 5 days after, on rate of pregnancy. It was used a random design with and arrangement of repeated measures over time, to analyze hormonal levels during the protocol. Overall pregnancy level was 31.2%, without differences between protocols (P=0.1056). No effect of weight, milking days and estradiol and progesterone levels on pregnancy rate were found (P>0.05). Progesterone levels were higher in prostaglandin injection (P=0.0006) and five days after ovulation (P=0.005) than in artificial insemination. No differences in estradiol levels were found during experiment. It was observed high variation of progesterone levels in prostaglandin injection (between 0.2 and 28.9 ng/ml) and estradiol levels in insemination (between 47.4 and 211.9 pg/ml). The use of progesterone does not improve pregnancy rates in FTAI during favorable breeding season in analyzed species. Individual hormonal variation must be considered and evaluated during the application of FTAI protocols.
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