DOI: 10.17151/vetzo.2019.13.2.5
Cómo citar
Cuevas Ramos G., Arguelles D., Vidal L., & Prades M. (2019). Desarrollo de un protocolo estandarizado y repetible para obtener plasma altamente concentrado rico en plaquetas en caballos. Revista Veterinaria y Zootecnia (On Line), 13(2), 52-62. https://doi.org/10.17151/vetzo.2019.13.2.5

Autores/as

Gabriel Cuevas Ramos
Université de Toulouse
gcuevasenvt@gmail.com
https://orcid.org/0000-0001-8851-8389
David Arguelles
Universitat Autònoma de Barcelona
david.arguelles@uab.cat
https://orcid.org/0000-0002-9198-5815
Luis Vidal
scientific@proteal.com
Marta Prades
Universitat Autònoma de Barcelona
marta.prades@uab.cat
https://orcid.org/0000-0003-2713-9232

Resumen

Introducción: el plasma rico en plaquetas (PRP) es un producto autólogo derivado de la sangre con varias aplicaciones terapéuticas. Se ha demostrado que los factores de crecimiento plaquetario estimulan la curación y reducen el dolor. Su uso en la industria equina, particularmente para problemas ortopédicos como la osteoartritis o la tendinitis ha ido en aumento. Sin embargo, el PRP carece de reproducibilidad y a veces, de calidad, ya que no solo los factores individuales (raza, edad, sexo) y ambientales (hora del día, estado de hidratación), sino también los métodos de laboratorio pueden afectar su calidad final. Objetivos: nuestro objetivo fue desarrollar y normalizar un protocolo para la preparación de PRP, que será repetible para uso equino, a través de un kit desechable estéril con una centrífuga estándar, que minimiza las variantes de laboratorio. Métodos y resultados: se estableció el protocolo final que resultó en un PRP con una concentración de plaquetas muy alta (6,4±1,3 veces mayor) y recuentos bajos de glóbulos blancos (3,7±1,8 x106/ml). El PRP final tenía concentraciones de factor de crecimiento derivado de plaquetas con un promedio de 37 ng/ml. Conclusión: El protocolo es altamente repetible y simple de realizar, y el kit es adecuado para usar en condiciones de campo porque no permite el contacto entre la muestra y el aire ambiente a lo largo del proceso.

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