RECORD OF Cletocamptus sinaloensis (COPEPODA: HARPACTICOIDA: CANTHOCAMPTIDAE) FROM THE CARIBBEAN COAST OF COLOMBIA*

Objectives: to report the first record of C. sinaloensis for Colombia. Scope: to present a brief description of this material in order to support the identification of the newly found material. Methodology: Water samples were taken at Ciénaga Grande de Santa Marta Colombia using a 25 L bucket. Samples were filtered with a standard zoo plankton net (45 μm mesh) and fixed and preserved in 70% ethanol. Harpacticoid copepods were separated with a brush. Dissected specimens and appendages were mounted in glycerine. The dissected appendages were photographed using a Kodak Easy Share C140 digital camera adapted to a compound microscope at a magnification of 1000X. Main results: Cletocamptus sinaloensis seems to be closely related to C. levis Gómez 2005, known from Brazil. The closeness of these species was assumed based on the general shape and number of setae of the mandibular palp (one-segmented with three setae), P2-P4 EXP3 and ENP2 with 5-5-4 and 3-3-2 elements respectively, length: width ratio of the caudal rami, and ornamentation of the anal operculum with two rows of strong spinules. Nevertheless, they can be separated by 1) the armature complement of the antennal exopod, 2) length of the exopodal lobe of the female P5, 3) relative length of the P3ENP, 4) number of setae on the female P6. Conclusions: The specimen from Colombia bears the diagnostic features of C. sinaloensis as originally described, but shows subtle differences in the length of P1ENP, shape of seminal receptacle and relative length of P6.

Here we present the first record of C. sinaloensis in northern Colombia (Magdalena department), and Caribbean Sea which expands the regional distributional range of this copepod in northern South America.The aim of this paper is to report the first record of C. sinaloensis for Colombia and to present a brief description of this material in order to support the identification of the newly found material.

MATERIALS AND METHODS
Water samples were taken at Cienaga Grande de Santa Marta Colombia (10°52´11.25"N and 74°19´31.64"W) in April of 2015.Water salinity was measured with a WTW 3111 conductivity meter.Water sam ples were collected using a 25 L bucket.Samples were filtered with a standard zoo plankton net (45 μm mesh size) and fixed and preserved in 70% ethanol.Harpacticoid copepods were separated from the sediment with a brush.Dissected specimens and appendages were mounted in glycerine.The dissected appendages were photographed using a Kodak Easy Share C140 digital camera adapted to a compound microscope at a magnification of 1000X.Identification of this species of Cletocamptus followed the keys and descriptions by GÓMEZ et al. (2004); GOMEZ (2005), WELLS (2007) and GÓMEZ & GEE (2009).The specimens were measured in lateral position, from the anterior end of the rostral area to the posterior margin of the caudal rami.Mor phological nomenclature follows HUYS & BOXSHALL (1991).The following abbreviations are used in the descriptions: P1-P6, first to sixth swimming legs; EXP, exopod; ENP, endopod.The specimens were deposited in the collection held at the Museo de Colecciones Biológicas from Universidad del Atlántico, (UARC), Barraquilla-Atlántico, Colombia.

Description of female
The studied specimens (2 adult females) agree with the descriptions and illustrations by GÓMEZ et al. (2004) and GÓMEZ (2005).Body length of the Colombian females ranged between 630 µm to700 µm (n = 2).Body shape (Fig. 1A) as for the genus.Ventral and dorsal surface of urosomites ornamented with transverse rows of spinules as illustrated (Figures 1B-C).Seminal receptacle as in figure 1D.Dorsal surface of anal somite (Figure 1E) ornamented with proximal transverse rows of minute spinules (arrowed in figure 1E) and transverse rows of stronger spinules.Anal operculum with two rows of strong spinules (Figure 1F).Caudal rami about 1.3 times as long as wide (Figure 2A), with seven elements.
Antenna (Figure 2C) with small coxa.Allobasis armed with two abexopodal setae.Free endopodal segment with inner strong spinules proximally and subdistally, with two lateral inner spines and five distal elements.Exopod one-segmented, with one lateral small and one apical long seta, and two spinules in each side (arrowed in Figure 2D).Mandibular palp (Figure 2E).represented by 1 small segment bearing 2 naked setae, accompanied by 1 slender seta nearby (arrowed in figure 2E).

Distribution and ecology
Cletocamptus was originally described from Mexico (GÓMEZ et al., 2004), but is also known from Brazil (GÓMEZ, 2005).In Colombia this species was found in Ciénaga Grande de Santa Marta, Magdalena, on the Caribbean coast of Colombia, and represents a range extension of the species to the Guajira province sensu MORRONE (2014) in the Caribbean coast of Colombia.Ciénaga Grande de Santa Marta is a shallow swamp (depth 0.5-1.5m),with following water chemistry at the date: 31.2°C, pH = 8.9, salinity = 15PSU, dissolved oxygen = 7.86mg/L.

DISCUSSION
The specimens of Cletocamptus sinaloensis examined (two adult females) agree with the descriptions and illustrations by GÓMEZ et al. (2004) andGÓMEZ (2005).This species can be easily recognized by a unique combination of characters including: 1) antennal exopod one-segmented with two setae, 2) mandibular palp with three elements, 3) arthrite of maxillule with a strong and spinulose lateral seta , 4) female P2-P4 EXP3 and ENP2 with 5-5-4 and 3-3-2 elements respectively, 5) anal operculum with two rows of strong spinules, 6) caudal rami of about 1.3-1.5 times as long as wide, and 7) caudal setae IV and V separated, not fused.These distinctive characteristics also were observed in the specimens from Colombia.
Female from Ciénaga Grande de Santa Marta Colombia.A. P5 (The arrow points at the seta 3).B.P6 Figure 4.